##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064012_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2269470
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29992861769488	32.0	32.0	32.0	32.0	32.0
2	31.40694126822562	32.0	32.0	32.0	32.0	32.0
3	31.48388918998709	32.0	32.0	32.0	32.0	32.0
4	31.56839967040763	32.0	32.0	32.0	32.0	32.0
5	31.50865708733757	32.0	32.0	32.0	32.0	32.0
6	35.10566960567886	36.0	36.0	36.0	36.0	36.0
7	35.11869202941656	36.0	36.0	36.0	36.0	36.0
8	35.07083415951742	36.0	36.0	36.0	36.0	36.0
9	35.177487695364995	36.0	36.0	36.0	36.0	36.0
10	35.031149563554486	36.0	36.0	36.0	36.0	36.0
11	35.178949270093895	36.0	36.0	36.0	36.0	36.0
12	35.069072074096596	36.0	36.0	36.0	36.0	36.0
13	35.113857420455	36.0	36.0	36.0	36.0	36.0
14	35.0609666574134	36.0	36.0	36.0	36.0	36.0
15	35.03094599179544	36.0	36.0	36.0	36.0	36.0
16	35.04386795154816	36.0	36.0	36.0	36.0	36.0
17	34.99680013395198	36.0	36.0	36.0	36.0	36.0
18	34.99710416969601	36.0	36.0	36.0	36.0	36.0
19	35.00037145236553	36.0	36.0	36.0	36.0	36.0
20	34.99134643771453	36.0	36.0	36.0	36.0	36.0
21	34.98129739542713	36.0	36.0	36.0	36.0	36.0
22	34.97176389201003	36.0	36.0	36.0	36.0	36.0
23	34.91583982163236	36.0	36.0	36.0	32.0	36.0
24	34.878275985141904	36.0	36.0	36.0	32.0	36.0
25	34.84912556676228	36.0	36.0	36.0	32.0	36.0
26	34.79006992822113	36.0	36.0	36.0	32.0	36.0
27	34.790078300219875	36.0	36.0	36.0	32.0	36.0
28	34.77861394951244	36.0	36.0	36.0	32.0	36.0
29	34.73345715078851	36.0	36.0	36.0	32.0	36.0
30	34.714520130250676	36.0	36.0	36.0	32.0	36.0
31	34.711686869621545	36.0	36.0	36.0	32.0	36.0
32	34.67217544184325	36.0	36.0	36.0	32.0	36.0
33	34.64818041216672	36.0	36.0	36.0	32.0	36.0
34	34.64367847999753	36.0	36.0	36.0	32.0	36.0
35	34.59241893481738	36.0	36.0	36.0	32.0	36.0
36	34.56576205017031	36.0	36.0	36.0	32.0	36.0
37	34.56406605947644	36.0	36.0	36.0	32.0	36.0
38	34.51223677774987	36.0	36.0	36.0	32.0	36.0
39	34.51776405945	36.0	36.0	36.0	32.0	36.0
40	34.489805108681765	36.0	36.0	36.0	32.0	36.0
41	34.47946613085875	36.0	36.0	36.0	32.0	36.0
42	34.393603792956064	36.0	36.0	36.0	32.0	36.0
43	34.39438238883969	36.0	36.0	36.0	32.0	36.0
44	34.30557090421993	36.0	36.0	36.0	32.0	36.0
45	34.2815591305459	36.0	36.0	36.0	32.0	36.0
46	34.2532177116243	36.0	36.0	36.0	32.0	36.0
47	34.22311817296549	36.0	36.0	36.0	32.0	36.0
48	34.1706147250239	36.0	36.0	36.0	32.0	36.0
49	34.143624282321426	36.0	36.0	36.0	32.0	36.0
50	33.53582642643437	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	7.0
19	4.0
20	27.0
21	80.0
22	248.0
23	714.0
24	1873.0
25	4131.0
26	8591.0
27	15862.0
28	26274.0
29	40383.0
30	58034.0
31	82503.0
32	119447.0
33	192434.0
34	397187.0
35	1321670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.619973269207634	18.178260806339246	11.95156376898003	26.25020215547309
2	16.358502587837997	20.1167501372572	36.25885782621608	27.265889448688718
3	17.830657662476835	24.096503929125053	28.854415716147702	29.218422692250414
4	11.97565951521721	15.690623802033073	35.851674620065474	36.482042062684236
5	14.363662000378943	36.733995161865984	33.06146368976016	15.840879147994908
6	34.79329498657393	35.91703951502247	16.181031132433798	13.108634365969793
7	30.525246213982214	30.45320292984835	20.55401505814796	18.467535798021476
8	28.76980087861924	32.30370967670866	18.864536653932415	20.06195279073969
9	27.441451389528424	13.479586805373486	18.420763898038235	40.65819790705985
10	16.244148915310355	26.194925556885785	30.674643338851553	26.886282188952304
11	38.07734845580686	20.75740150784104	22.030694391201468	19.13455564515063
12	24.839675941388855	23.69182882669315	28.137235807894896	23.331259424023095
13	29.365682263020926	18.773480234522545	25.544380121808608	26.316457380647922
14	23.96962454570771	19.594582685603296	24.50204101056461	31.933751758124384
15	25.52894379568702	27.08368493434994	21.877727891438237	25.509643378524803
16	26.443167777948158	25.343386861674094	23.15175645926858	25.061688901109164
17	24.32131731197152	25.761389223034453	24.513652967432925	25.403640497561103
18	25.449026334829426	24.24561548840124	25.87617527647473	24.429182900294606
19	25.940869868678533	24.386409331874546	25.037090173956994	24.635630625489927
20	26.181836608348704	23.557153589738505	24.50094846560077	25.760061336312024
21	27.8398921334056	23.606789250353607	23.90857777366522	24.64474084257558
22	26.452751722980132	23.533037904461352	24.816554004482988	25.197656368075528
23	24.778910800256977	23.322916694206427	25.55817587199695	26.33999663353964
24	25.190595832844963	24.591643305770265	24.935293034208108	25.282467827176664
25	25.18924363793712	23.934743056513337	25.204005060303714	25.67200824524583
26	25.07016360270546	25.028345258244798	25.437979958040646	24.463511181009096
27	25.750873056966277	24.187632886430986	24.60079539069988	25.46069866590286
28	24.893362756986942	24.024181096477623	25.723679108265884	25.358777038269555
29	24.783986752568165	24.14206090452146	25.545855088148034	25.52809725476234
30	24.505965951904003	24.6243684392816	25.840781042206277	25.028884566608117
31	25.515025298483323	24.284737272099928	24.468141361279617	25.732096068137132
32	25.303493513280568	24.223210831380545	24.263617880626033	26.20967777471285
33	24.724473568366378	23.95778940795802	25.012216977037372	26.305520046638225
34	25.69957455996369	24.06360229398325	25.211959910373377	25.02486323567968
35	26.32265654949965	23.790588743329764	25.350950246980492	24.535804460190093
36	24.70887780579398	24.778281795179353	24.683055115140117	25.82978528388655
37	26.05208018912613	24.464777902383243	24.59463687298541	24.888505035505215
38	24.967291401990323	24.07348406830181	25.162551486572177	25.796673043135687
39	25.950566321080963	24.048816771423436	24.277777581934128	25.722839325561473
40	26.089297666673573	24.405828623247956	24.990030496765474	24.514843213312997
41	24.594792885328467	24.986615375463504	25.59272405200505	24.825867687202976
42	26.530685085429127	24.98280401423268	24.66483800962799	23.821672890710204
43	25.367569785052034	23.67921855370923	25.078246416615958	25.87496524462278
44	24.84697733136221	24.748759052363255	24.514075089174177	25.890188527100356
45	25.041331782289905	24.82233502538071	24.747074168076704	25.38925902425268
46	24.8195268371073	24.706195463131834	24.74303256602588	25.731245133734987
47	25.197495395299242	24.379268711829454	25.174405795313344	25.248830097557967
48	26.323169057807306	25.014860631729974	23.8755822525501	24.78638805791262
49	25.031780757935273	25.328812424128262	24.184837328785985	25.45456948915048
50	25.146974454513487	25.28502601079913	24.228202351062507	25.339797183624874
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	34.0
2	43.0
3	127.5
4	212.0
5	285.0
6	358.0
7	335.5
8	313.0
9	434.0
10	555.0
11	855.0
12	1155.0
13	2275.0
14	3395.0
15	4575.5
16	5756.0
17	6342.5
18	6929.0
19	7158.0
20	7387.0
21	8090.0
22	8793.0
23	9606.0
24	10419.0
25	11761.0
26	13103.0
27	16515.5
28	19928.0
29	22630.5
30	25333.0
31	27423.5
32	29514.0
33	34092.0
34	38670.0
35	45291.5
36	51913.0
37	64885.0
38	77857.0
39	85715.0
40	93573.0
41	103158.5
42	112744.0
43	113387.5
44	114031.0
45	127546.0
46	141061.0
47	152102.0
48	163143.0
49	170752.5
50	178362.0
51	170765.0
52	163168.0
53	162181.5
54	161195.0
55	165720.5
56	170246.0
57	167732.0
58	165218.0
59	151707.5
60	138197.0
61	121322.0
62	104447.0
63	91346.0
64	78245.0
65	67410.0
66	56575.0
67	48762.0
68	40949.0
69	37920.5
70	34892.0
71	26320.0
72	17748.0
73	15399.5
74	13051.0
75	10128.0
76	7205.0
77	6257.0
78	5309.0
79	4421.0
80	3533.0
81	2864.0
82	2195.0
83	1828.5
84	1462.0
85	1019.5
86	577.0
87	417.0
88	257.0
89	192.0
90	127.0
91	118.5
92	110.0
93	87.5
94	65.0
95	56.5
96	48.0
97	41.0
98	34.0
99	39.5
100	45.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007799177781596584
2	3.525052104676422E-4
3	3.525052104676422E-4
4	0.0
5	0.0
6	1.762526052338211E-4
7	4.4063151308455275E-5
8	0.0
9	0.0013659576905621137
10	8.371998748606502E-4
11	0.0
12	1.762526052338211E-4
13	3.525052104676422E-4
14	2.6437890785073167E-4
15	0.00392162046645252
16	7.931367235521951E-4
17	0.0
18	1.762526052338211E-4
19	4.4063151308455275E-5
20	6.609472696268292E-4
21	0.0
22	4.4063151308455275E-5
23	0.0010575156314029267
24	3.525052104676422E-4
25	0.0018065892036466663
26	0.005772272821407642
27	0.006389156939726015
28	0.004141936222994796
29	0.0021590944141143087
30	0.004053809920377885
31	0.007226356814586665
32	0.0028200416837411376
33	0.003833494163835609
34	0.0024234733219650403
35	0.0026437890785073167
36	0.006521346393651381
37	0.0037453678612186987
38	0.008327935597298048
39	0.003789431012527154
40	0.0021590944141143087
41	0.0033047363481341456
42	0.0019828418088804877
43	0.0016303365984128453
44	0.0015422102957959346
45	0.0013218945392536583
46	8.371998748606502E-4
47	0.0022031575654227636
48	0.0022472207167312194
49	6.609472696268292E-4
50	0.0012337682366367478
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2269470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.41562053756243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.76255031075138	29.3067493194025
2	15.087224600986787	12.49693538078891
3	5.761130928666349	7.158024372265805
4	2.7418408718647735	4.542201644941232
5	1.4728865598691065	3.050025542920732
6	0.9662196680020292	2.4009952275540956
7	0.6206451940277423	1.7993084091020346
8	0.4188426616448124	1.3877302991699365
9	0.326567254632061	1.2172486948051466
>10	1.598302483053764	11.668286746233026
>50	0.11021120691439217	3.130876164968879
>100	0.10511126771573559	9.232890288353827
>500	0.01787213399426598	5.170661241230587
>1k	0.01059485787684031	7.4380666682632715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3318	0.1462015360414546	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3313	0.14598122028491234	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	3311	0.1458930939822954	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3306	0.14567277822575314	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3286	0.14479151519958405	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3275	0.14430682053519103	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2992	0.1318369487148982	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2889	0.1272984441301273	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2876	0.1267256231631174	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2850	0.12557998122909755	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2817	0.12412589723591852	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2799	0.12333276051236632	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	2643	0.11645890890824728	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	2449	0.10791065755440697	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	2449	0.10791065755440697	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	2274	0.1001996060754273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.525052104676422E-4
2	0.0	0.0	0.0	0.0	3.525052104676422E-4
3	0.0	0.0	0.0	0.0	3.525052104676422E-4
4	0.0	0.0	0.0	0.0	3.525052104676422E-4
5	0.0	0.0	0.0	0.0	3.965683617760975E-4
6	0.0	0.0	0.0	0.0	4.406315130845528E-4
7	0.0	0.0	0.0	0.0	4.8469466439300804E-4
8	0.0	0.0	0.0	0.0	4.8469466439300804E-4
9	0.0	0.0	0.0	0.0	4.8469466439300804E-4
10	0.0	0.0	0.0	4.4063151308455275E-5	4.8469466439300804E-4
11	0.0	0.0	0.0	4.4063151308455275E-5	4.8469466439300804E-4
12	0.0	0.0	0.0	4.4063151308455275E-5	0.0013218945392536583
13	0.0	0.0	0.0	4.4063151308455275E-5	0.0013659576905621137
14	0.0	0.0	0.0	4.4063151308455275E-5	0.0013659576905621137
15	0.0	0.0	0.0	4.4063151308455275E-5	0.0017625260523382112
16	0.0	0.0	0.0	4.4063151308455275E-5	0.001894715506263577
17	0.0	0.0	0.0	4.4063151308455275E-5	0.0019387786575720321
18	0.0	0.0	0.0	4.4063151308455275E-5	0.0021590944141143087
19	0.0	0.0	0.0	4.4063151308455275E-5	0.002203157565422764
20	0.0	0.0	0.0	4.4063151308455275E-5	0.0022912838680396743
21	0.0	0.0	0.0	1.762526052338211E-4	0.002511599624581951
22	0.0	0.0	0.0	4.406315130845528E-4	0.002511599624581951
23	0.0	0.0	0.0	7.490735722437398E-4	0.002511599624581951
24	0.0	0.0	0.0	0.0012337682366367478	0.0025997259271988613
25	0.0	0.0	0.0	0.0014981471444874795	0.0025997259271988613
26	0.0	0.0	0.0	0.001894715506263577	0.002687852229815772
27	0.0	0.0	0.0	0.002555662775890406	0.0027319153811242274
28	0.0	0.0	0.0	0.005816335972716097	0.0027319153811242274
29	0.0	0.0	0.0	0.011897050853282925	0.0028200416837411376
30	0.0	0.0	0.0	0.02498380679189414	0.0028200416837411376
31	0.0	0.0	0.0	0.051157318669116575	0.002864104835049593
32	0.0	0.0	0.0	0.07451078886259788	0.002864104835049593
33	0.0	0.0	0.0	0.09790832220738763	0.0029522311376665037
34	0.0	0.0	0.0	0.12505122341339608	0.0032166100455172353
35	0.0	0.0	0.0	0.15620387138847397	0.0032166100455172353
36	0.0	0.0	0.0	0.20595116921571996	0.0032166100455172353
37	0.0	0.0	0.0	0.2770690954275668	0.0032166100455172353
38	0.0	0.0	0.0	0.37779745931869557	0.0035691152559848777
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGC	45	0.00139933	24.44223	6
GTATCAA	4795	0.0	22.481695	1
GATATAC	375	0.0	18.773285	1
ACCGTAT	85	1.4325269E-4	18.116005	8
AACGCAG	6020	0.0	17.759188	6
ATACCGT	905	0.0	17.744247	6
ACGATAC	75	0.0012921286	17.598404	3
CGTCGTA	845	0.0	17.44259	10
TACCGTC	900	0.0	17.10956	7
ATAGTAC	260	0.0	16.921543	3
CGTCTTA	290	0.0	16.688877	15
TCTAGAT	765	0.0	16.678228	2
GGTATCA	2445	0.0	16.646175	1
TACGCTC	120	6.407641E-6	16.501049	34
CCGTCGT	870	0.0	16.435656	9
GTCCTAT	925	0.0	16.41077	1
GTCGTAG	880	0.0	16.248886	11
TATACTG	325	0.0	16.24468	5
TAACGGC	285	0.0	16.212986	36
ACCGTCG	900	0.0	16.13187	8
>>END_MODULE