FastQCFastQC Report
Thu 2 Feb 2017
SRR4064011_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064011_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861197
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38980.20943511084533234No Hit
TATCAACGCAGAGTACTTTTTTTTT25110.1349131768426448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA307.722043E-419.0005369
CGTTATT1400.014.2442762
CGTCTTA1001.4399484E-712.3520115
TCTAGGA1850.012.3193742
CCGATAA1251.4097168E-912.1603449
GCGTTAT1650.012.0873511
CTAGTAG550.003076225312.0870283
GGTATCA8450.012.0260471
GTATCAA21450.011.9545241
TTTTCGG1750.011.94480116
GTTATTC2150.011.9264023
TAGACAG1950.011.6910685
GGTACCT1900.011.5000168
CTCTTAA4400.011.43981551
AATTCCG1501.7644197E-1011.398795
CGAACGT752.0778347E-411.3978714
ACTAGAC1251.8409992E-811.3963383
GGTCCAA3450.011.29078211
CCGGTCC3450.011.29017459
ACGAACG1354.7166395E-911.26109115
Produced by FastQC (version 0.11.5)