FastQCFastQC Report
Thu 2 Feb 2017
SRR4064011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861197
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25350.025.8616311
ATGCGTA551.593904E-424.00036622
GGTATCA11450.021.1351261
ACTATAC1653.6379788E-1219.9992313
CCTTATA2250.019.5542772
GTATAGT1504.656613E-1019.0664461
TACTATA2050.018.242712
GATATAC3650.018.0819821
AACGCAG37300.017.4577736
TCAACGC38200.017.0464634
GGCGTTA4550.016.92242642
TATACCG1053.7773534E-516.7612615
CAACGCA39000.016.5839775
TATCAAC39650.016.4781172
ATCAACG39600.016.4438113
TCTAGCG5150.016.23325728
ATAATAC2450.016.1626433
ATACGAA5350.016.03633540
ATTAGAC1259.4491115E-615.8393913
GTATAGG1958.931238E-1015.7946881