FastQCFastQC Report
Thu 2 Feb 2017
SRR4064010_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064010_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094004
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40210.36754893035126013No Hit
TATCAACGCAGAGTACTTTTTTTTT29850.2728509219344719No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24120.2204745138043371No Hit
GGTATCAACGCAGAGTACTTTTTTT14670.1340945736944289No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14520.13272346353395417No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14420.13180939009363768No Hit
GTACATGGGGTGGTATCAACGCAAA12030.1099630348700736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCA453.5369005E-516.8891339
ATTCGAA655.4488657E-513.15403651
CCGTCGT1600.013.062699
CCCTTAA801.9975305E-613.062691
CGTCGTA1650.012.6662710
CGAACGT751.4801964E-512.6645334
CCAACGA1155.304173E-912.39318419
CGGTCCA1251.4078978E-912.15961910
AGGCCCG2450.012.02003210
ATCCCCG1353.7107384E-1011.96258812
AATCGAA802.862775E-511.8757157
GTCCTAA802.8641152E-511.8751731
ATCCCGT1201.0002623E-811.87462810
GCCGCCT2650.011.83198418
TGAGTTA2850.011.66630212
CGGACCA1552.5465852E-1111.6453299
TTTAGGA1406.82121E-1011.5327162
ATACCGT1900.011.5001666
CGCCAGT1001.9241543E-611.40172918
CCGAGCC2750.011.39964314
Produced by FastQC (version 0.11.5)