FastQCFastQC Report
Thu 2 Feb 2017
SRR4064010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094004
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11030.10082230046690871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT2450.020.6516676
CGTCGTA2250.020.53194610
TAATACT1401.8553692E-1020.4271934
TAACGGC550.004481537720.00139236
TACCGTC2650.019.093057
ACGTCTC708.121343E-418.85759442
GTATCAA29400.018.7819041
TTAGGAC1557.221388E-1018.4503673
GTATAGA2150.018.4182011
TAGATCG600.007408687418.33460827
GGCGAAT851.4304131E-418.1180837
GTCGTAG2600.017.7680311
GTATTAC750.001290952717.5996171
CCGTCGT2650.017.4327859
GACGGCC3100.017.03110711
ACCGTCG2850.016.981318
GCATTCG3150.016.76307122
CGAGCCG3150.016.76077315
ATATTAC1206.4067444E-616.4996411
GCACGTT800.00198908516.49888613
Produced by FastQC (version 0.11.5)