##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064010_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1094004
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25937839349765	32.0	32.0	32.0	32.0	32.0
2	31.3804912961927	32.0	32.0	32.0	32.0	32.0
3	31.456511127930064	32.0	32.0	32.0	32.0	32.0
4	31.544642432751616	32.0	32.0	32.0	32.0	32.0
5	31.490512831762956	32.0	32.0	32.0	32.0	32.0
6	35.065221882186904	36.0	36.0	36.0	36.0	36.0
7	35.08376934636436	36.0	36.0	36.0	36.0	36.0
8	35.027955108025196	36.0	36.0	36.0	36.0	36.0
9	35.13618505965243	36.0	36.0	36.0	36.0	36.0
10	34.98866366119319	36.0	36.0	36.0	36.0	36.0
11	35.135192375896246	36.0	36.0	36.0	36.0	36.0
12	35.04221465369414	36.0	36.0	36.0	36.0	36.0
13	35.078575581076485	36.0	36.0	36.0	36.0	36.0
14	35.02485822720941	36.0	36.0	36.0	36.0	36.0
15	34.997020120584565	36.0	36.0	36.0	36.0	36.0
16	35.009276931345774	36.0	36.0	36.0	36.0	36.0
17	34.96885660381498	36.0	36.0	36.0	36.0	36.0
18	34.965910545116834	36.0	36.0	36.0	36.0	36.0
19	34.96282554725577	36.0	36.0	36.0	36.0	36.0
20	34.95369760988077	36.0	36.0	36.0	36.0	36.0
21	34.946294529087645	36.0	36.0	36.0	36.0	36.0
22	34.93758980771551	36.0	36.0	36.0	36.0	36.0
23	34.88781576666996	36.0	36.0	36.0	32.0	36.0
24	34.85357274744882	36.0	36.0	36.0	32.0	36.0
25	34.82967795364551	36.0	36.0	36.0	32.0	36.0
26	34.76783448689402	36.0	36.0	36.0	32.0	36.0
27	34.761073085655994	36.0	36.0	36.0	32.0	36.0
28	34.73859601975861	36.0	36.0	36.0	32.0	36.0
29	34.70400565263016	36.0	36.0	36.0	32.0	36.0
30	34.68657884249052	36.0	36.0	36.0	32.0	36.0
31	34.67785035520894	36.0	36.0	36.0	32.0	36.0
32	34.63508085893653	36.0	36.0	36.0	32.0	36.0
33	34.60117239059455	36.0	36.0	36.0	32.0	36.0
34	34.586940267128824	36.0	36.0	36.0	32.0	36.0
35	34.535480674659325	36.0	36.0	36.0	32.0	36.0
36	34.513872892603686	36.0	36.0	36.0	32.0	36.0
37	34.501205662867775	36.0	36.0	36.0	32.0	36.0
38	34.44596089228193	36.0	36.0	36.0	32.0	36.0
39	34.442228730425114	36.0	36.0	36.0	32.0	36.0
40	34.43024796984289	36.0	36.0	36.0	32.0	36.0
41	34.39668502126135	36.0	36.0	36.0	32.0	36.0
42	34.332963133590006	36.0	36.0	36.0	32.0	36.0
43	34.310869064464114	36.0	36.0	36.0	32.0	36.0
44	34.245111535241186	36.0	36.0	36.0	32.0	36.0
45	34.20645811167052	36.0	36.0	36.0	32.0	36.0
46	34.16273706494675	36.0	36.0	36.0	32.0	36.0
47	34.12128931886904	36.0	36.0	36.0	32.0	36.0
48	34.05656926300087	36.0	36.0	36.0	32.0	36.0
49	34.02221472682001	36.0	36.0	36.0	32.0	36.0
50	33.43177904285542	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	24.0
21	37.0
22	114.0
23	391.0
24	959.0
25	2252.0
26	4614.0
27	8358.0
28	13989.0
29	21049.0
30	29546.0
31	41914.0
32	59611.0
33	93616.0
34	188525.0
35	628999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91397714796596	18.44398106968874	12.478300257700152	26.163741524645147
2	15.52119239379123	21.00363710819277	37.25864967216666	26.216520825849337
3	17.737982828106475	25.048149126551532	29.346881207169673	27.866986838172313
4	12.148127429150167	16.61429025853653	36.529025488023805	34.70855682428949
5	13.903696878622016	37.398217922420756	33.41185224185652	15.286232957100706
6	32.89844726202762	36.64935105296786	17.100867182265496	13.351334502739023
7	29.229353118775727	31.129530723407523	21.188790158710717	18.452325999106034
8	27.73801558312401	32.93424886929115	19.903309311483323	19.424426236101514
9	27.644046633137904	13.970014241498522	18.729398730879552	39.656540394484026
10	15.69781525110307	27.177230567288824	31.996365607458184	25.128588574149923
11	36.635058007100525	21.3715854786637	22.960610747309882	19.0327457669259
12	24.447213076392913	24.117780406251544	29.25616223736337	22.178844279992173
13	29.549607358677004	19.896051283316925	25.432792108965163	25.121549249040903
14	23.513850980162744	19.767696951193873	25.983499116089366	30.734952952554018
15	24.89745996893862	27.40677701165396	22.99273375790589	24.703029261501534
16	25.079982010903144	26.04360527826427	24.42412952149734	24.452283189335244
17	23.559511665405246	25.902281892936408	25.77220924237937	24.76599719927898
18	24.11604366353992	25.434962641750197	26.832766302072574	23.616227392637306
19	25.20191882296591	24.853382620173235	26.252554835265684	23.69214372159517
20	25.591754243400093	24.410211034257078	25.670273941423755	24.327760780919075
21	26.704289929470093	24.14780933159294	25.151096339684315	23.996804399252653
22	25.48420799577332	24.396185385232034	25.71492034299723	24.404686275997413
23	24.313249091401033	24.318550775508413	25.966003407703163	25.402196725387387
24	24.254844606946985	25.089031078610603	26.028702010968924	24.62742230347349
25	24.599240391778682	24.576022523160738	26.15620872315434	24.66852836190624
26	24.301806969543783	25.377194722519064	26.339869699277195	23.98112860865996
27	24.733849515051237	25.065314965308566	25.645608037077327	24.55522748256287
28	24.08652967201726	25.212622033712385	26.26247760429997	24.43837068997038
29	24.12153089141728	24.914600581182235	26.14826454303884	24.815603984361644
30	24.149278671680893	25.45253273903656	26.080895540397492	24.317293048885052
31	24.746230944466994	24.9975317851227	25.41319745353357	24.84303981687674
32	24.427702115328888	25.260542594063622	25.315937318036102	24.995817972571384
33	24.271102713385567	24.806412010819535	25.871637291386147	25.050847984408755
34	24.672614422698977	24.99090468660018	26.074023967750488	24.262456922950353
35	25.106036008365873	24.873762303833384	25.900026326181386	24.120175361619353
36	24.3541622483381	25.454874543845662	25.564296736165453	24.626666471650786
37	24.99341843058821	25.30713990588356	25.41061680274671	24.288824860781524
38	24.380355299832797	25.072561941615863	25.858653451480173	24.688429307071168
39	24.802685344141988	25.04045019004733	25.37310158345354	24.783762882357134
40	25.05509774078695	25.236547787177834	25.692686694729723	24.015667777305488
41	23.99635453516667	25.514505988325002	26.228241931621028	24.2608975448873
42	25.04862995166256	25.71418385778116	25.58575325235654	23.65143293819974
43	24.47554365213576	24.77380963262918	25.910657318622654	24.8399893966124
44	24.121631935970107	25.40811653499258	25.48261454182812	24.987636987209193
45	24.43929915136181	25.483094878554425	25.5127112911659	24.564894678917867
46	24.107214677986676	25.354755793461013	25.408686718001594	25.129342810550714
47	24.26511587942032	25.34493254916443	25.837079586318605	24.552871985096647
48	24.885715565444006	25.475579557597435	24.99430977320447	24.64439510375409
49	24.190972752260283	25.79243673897325	25.314739775813322	24.701850732953137
50	24.35227808714538	25.90412326277802	25.046161383854304	24.6974372662223
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	168.0
1	108.0
2	48.0
3	131.0
4	214.0
5	258.5
6	303.0
7	286.0
8	269.0
9	379.0
10	489.0
11	752.0
12	1015.0
13	1733.5
14	2452.0
15	3317.0
16	4182.0
17	4513.5
18	4845.0
19	4880.5
20	4916.0
21	5355.5
22	5795.0
23	6505.0
24	7215.0
25	7818.0
26	8421.0
27	10207.5
28	11994.0
29	14239.0
30	16484.0
31	18672.5
32	20861.0
33	23961.5
34	27062.0
35	30390.5
36	33719.0
37	38522.5
38	43326.0
39	46550.5
40	49775.0
41	53456.5
42	57138.0
43	58589.0
44	60040.0
45	65307.0
46	70574.0
47	74447.0
48	78320.0
49	80209.5
50	82099.0
51	79313.0
52	76527.0
53	75808.5
54	75090.0
55	74522.0
56	73954.0
57	71463.5
58	68973.0
59	62745.5
60	56518.0
61	49885.5
62	43253.0
63	37678.0
64	32103.0
65	27503.0
66	22903.0
67	20223.5
68	17544.0
69	15607.5
70	13671.0
71	10723.0
72	7775.0
73	6573.5
74	5372.0
75	4229.5
76	3087.0
77	2648.5
78	2210.0
79	1844.5
80	1479.0
81	1153.0
82	827.0
83	674.0
84	521.0
85	376.5
86	232.0
87	153.0
88	74.0
89	62.5
90	51.0
91	41.0
92	31.0
93	29.5
94	28.0
95	28.5
96	29.0
97	22.0
98	15.0
99	14.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008866512371069942
2	2.742220320949467E-4
3	6.398514082215422E-4
4	0.0
5	0.0
6	9.140734403164887E-5
7	9.140734403164887E-5
8	0.0
9	0.0016453321925696796
10	0.0010054807843481377
11	0.0
12	1.8281468806329775E-4
13	2.742220320949467E-4
14	1.8281468806329775E-4
15	0.003747701105297604
16	7.31258752253191E-4
17	0.0
18	1.8281468806329775E-4
19	0.0
20	0.0010054807843481377
21	0.0
22	9.140734403164887E-5
23	0.0010968881283797867
24	3.656293761265955E-4
25	0.0017367395366013286
26	0.006672736114310368
27	0.0067641434583420165
28	0.004021923137392551
29	0.0024679982888545196
30	0.004570367201582444
31	0.008775105027038294
32	0.0033820717291710082
33	0.005210218609803986
34	0.003107849697076062
35	0.003290664385139359
36	0.0069469581464053146
37	0.003656293761265955
38	0.010146215187513026
39	0.006032884706088826
40	0.0035648864172343063
41	0.003839108449329253
42	0.0025594056328861687
43	0.0012797028164430843
44	0.0013711101604747331
45	0.001462517504506382
46	0.0010968881283797867
47	0.0020109615686962753
48	0.0021023689127279243
49	4.5703672015824436E-4
50	0.001462517504506382
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1094004.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.2061367627851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.81299886899869	48.05232325354948
2	12.482937637247977	15.030989011789384
3	3.6065061966726786	6.514014159381216
4	1.4005934629083039	3.372972863076805
5	0.6991971281538911	2.104797896088988
6	0.4230036609560023	1.5280449757605512
7	0.2981501293343935	1.2565327223783995
8	0.19887870992190004	0.9578975047011344
9	0.1624015690266766	0.879981396777988
>10	0.7883148636586947	8.596306296267567
>50	0.06000629106834509	2.510055595948232
>100	0.06226866233140524	7.2121784808360285
>500	0.004589825536340599	1.8823064309955828
>1k	1.5299418454468663E-4	0.10159941244864831
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1103	0.10082230046690871	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8281468806329775E-4
2	0.0	0.0	0.0	0.0	1.8281468806329775E-4
3	0.0	0.0	0.0	0.0	1.8281468806329775E-4
4	0.0	0.0	0.0	0.0	1.8281468806329775E-4
5	0.0	0.0	0.0	0.0	1.8281468806329775E-4
6	0.0	0.0	0.0	0.0	2.742220320949466E-4
7	0.0	0.0	0.0	0.0	3.656293761265955E-4
8	0.0	0.0	0.0	0.0	3.656293761265955E-4
9	0.0	0.0	0.0	0.0	4.570367201582444E-4
10	0.0	0.0	0.0	0.0	4.570367201582444E-4
11	0.0	0.0	0.0	0.0	4.570367201582444E-4
12	0.0	0.0	0.0	0.0	7.31258752253191E-4
13	0.0	0.0	0.0	0.0	7.31258752253191E-4
14	0.0	0.0	0.0	9.140734403164887E-5	7.31258752253191E-4
15	0.0	0.0	0.0	9.140734403164887E-5	8.226660962848399E-4
16	0.0	0.0	0.0	2.742220320949466E-4	8.226660962848399E-4
17	0.0	0.0	0.0	2.742220320949466E-4	8.226660962848399E-4
18	0.0	0.0	0.0	3.656293761265955E-4	8.226660962848399E-4
19	0.0	0.0	0.0	3.656293761265955E-4	8.226660962848399E-4
20	0.0	0.0	0.0	3.656293761265955E-4	0.0011882954724114355
21	0.0	0.0	0.0	6.398514082215422E-4	0.0012797028164430843
22	0.0	0.0	0.0	0.0010054807843481377	0.0013711101604747331
23	0.0	0.0	0.0	0.0018281468806329777	0.0013711101604747331
24	0.0	0.0	0.0	0.0032906643851393596	0.0013711101604747331
25	0.0	0.0	0.0	0.004021923137392551	0.0013711101604747331
26	0.0	0.0	0.0	0.004570367201582444	0.0013711101604747331
27	0.0	0.0	0.0	0.005850070018025528	0.0013711101604747331
28	0.0	0.0	0.0	0.011608732692019407	0.0013711101604747331
29	0.0	0.0	0.0	0.021480725847437487	0.0013711101604747331
30	0.0	0.0	0.0	0.04067626809408375	0.0013711101604747331
31	0.0	0.0	0.0	0.08162675822026245	0.0013711101604747331
32	0.0	0.0	0.0	0.12742183758011855	0.001462517504506382
33	0.0	0.0	0.0	0.17029188193096187	0.001462517504506382
34	0.0	0.0	0.0	0.21325333362583684	0.0016453321925696798
35	0.0	0.0	0.0	0.26352737284324373	0.0018281468806329777
36	0.0	0.0	0.0	0.32998051195425243	0.0018281468806329777
37	0.0	0.0	0.0	0.440217768856421	0.0018281468806329777
38	0.0	0.0	0.0	0.5836358916420781	0.0019195542246646265
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	245	0.0	20.651667	6
CGTCGTA	225	0.0	20.531946	10
TAATACT	140	1.8553692E-10	20.427193	4
TAACGGC	55	0.0044815377	20.001392	36
TACCGTC	265	0.0	19.09305	7
ACGTCTC	70	8.121343E-4	18.857594	42
GTATCAA	2940	0.0	18.781904	1
TTAGGAC	155	7.221388E-10	18.450367	3
GTATAGA	215	0.0	18.418201	1
TAGATCG	60	0.0074086874	18.334608	27
GGCGAAT	85	1.4304131E-4	18.11808	37
GTCGTAG	260	0.0	17.76803	11
GTATTAC	75	0.0012909527	17.599617	1
CCGTCGT	265	0.0	17.432785	9
GACGGCC	310	0.0	17.031107	11
ACCGTCG	285	0.0	16.98131	8
GCATTCG	315	0.0	16.763071	22
CGAGCCG	315	0.0	16.760773	15
ATATTAC	120	6.4067444E-6	16.499641	1
GCACGTT	80	0.001989085	16.498886	13
>>END_MODULE