FastQCFastQC Report
Thu 2 Feb 2017
SRR4064009_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064009_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009340
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21880.2167753185249767No Hit
TATCAACGCAGAGTACTTTTTTTTT16380.1622842649652248No Hit
GCGCAAGACGGACCAGAGCGAAAGC12760.12641924425862444No Hit
GAATAGGACCGCGGTTCTATTTTGT11670.11562010818950998No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11550.11443121247547902No Hit
GTATCTGATCGTCTTCGAACCTCCG11330.11225157033308895No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11270.11165712247607348No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11150.11046822676204253No Hit
GAACTACGACGGTATCTGATCGTCT10740.10640616640577011No Hit
GAATAACGCCGCCGCATCGCCAGTC10340.1024431806923336No Hit
CTATTGGAGCTGGAATTACCGCGGC10180.100857986406959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT250.00603573418.9972256
TGCTCGA453.535315E-516.8897710
GCGTTAT755.8345904E-815.2007931
TCGAACG602.5736435E-514.2457993
AACCGCG759.657324E-713.9340617
AGGCCCG2200.013.81890310
CACCGTG551.9582108E-413.8189037
GAACGTC707.267463E-613.5694465
CGAACGA852.6925045E-713.4131316
AAACGTA852.6941234E-713.41246418
GTTTAGC500.001497201613.30201314
TTAGGGT500.001500562313.2980574
ACGGTAT1800.013.1944799
CAAATCG655.4435826E-513.15518613
CGAACGT655.4562606E-513.1519244
TGGGTAT802.0006992E-613.0605916
GTTGGAC1102.75395E-912.95072653
GTATCAA15500.012.7490531
GGTATCA5000.012.7306631
AATCGCT751.4750665E-512.66858415
Produced by FastQC (version 0.11.5)