##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064009_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1009340
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.200505280678463	32.0	32.0	32.0	32.0	32.0
2	30.94019953633067	32.0	32.0	32.0	32.0	32.0
3	30.951241405274732	32.0	32.0	32.0	32.0	32.0
4	30.969203638020886	32.0	32.0	32.0	32.0	32.0
5	30.9393970317237	32.0	32.0	32.0	32.0	32.0
6	34.506118849941544	36.0	36.0	36.0	32.0	36.0
7	34.44992569401787	36.0	36.0	36.0	32.0	36.0
8	34.42466166009471	36.0	36.0	36.0	32.0	36.0
9	34.509860899201456	36.0	36.0	36.0	32.0	36.0
10	34.32029940357065	36.0	36.0	36.0	32.0	36.0
11	34.47605266808013	36.0	36.0	36.0	32.0	36.0
12	34.36150157528682	36.0	36.0	36.0	32.0	36.0
13	34.41311451047219	36.0	36.0	36.0	32.0	36.0
14	34.339381179780844	36.0	36.0	36.0	32.0	36.0
15	34.30098579269622	36.0	36.0	36.0	32.0	36.0
16	34.302489745774466	36.0	36.0	36.0	32.0	36.0
17	34.22959359581509	36.0	36.0	36.0	32.0	36.0
18	34.219900132760024	36.0	36.0	36.0	32.0	36.0
19	34.22854736758674	36.0	36.0	36.0	32.0	36.0
20	34.18839142409892	36.0	36.0	36.0	32.0	36.0
21	34.172934789070084	36.0	36.0	36.0	32.0	36.0
22	34.15401945825985	36.0	36.0	36.0	32.0	36.0
23	34.124348584223355	36.0	36.0	36.0	32.0	36.0
24	34.10792200844116	36.0	36.0	36.0	32.0	36.0
25	33.69777478352191	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	10.0
5	11.0
6	38.0
7	21.0
8	52.0
9	65.0
10	87.0
11	33.0
12	61.0
13	61.0
14	88.0
15	165.0
16	308.0
17	460.0
18	582.0
19	751.0
20	940.0
21	1442.0
22	2122.0
23	3282.0
24	4957.0
25	6933.0
26	10112.0
27	13179.0
28	18511.0
29	25482.0
30	34218.0
31	47554.0
32	68569.0
33	98063.0
34	216084.0
35	455097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.300967428934456	17.408963460503507	11.412212340789504	25.877856769772528
2	16.506057759758402	19.449530558296775	38.27604519639665	25.76836648554817
3	19.067179484130996	23.54075888266031	28.577387286169188	28.81467434703951
4	12.303491405489114	15.13830776958844	36.15768046788539	36.40052035703706
5	14.134414264807734	36.91948401441462	33.871090796856684	15.075010923920958
6	34.072393258092966	36.093479057876955	16.600905658881697	13.233222025148383
7	30.15138121984961	30.571356088743723	20.499688834099025	18.77757385730764
8	28.063397800185136	33.3213081544588	19.152442928302843	19.462851117053223
9	27.183229404248948	13.97595902706121	18.63081237157547	40.20999919711437
10	15.529114611677963	26.72035480164831	31.760358211677563	25.990172374996156
11	37.5550894416612	20.989002928190924	22.477086955573576	18.9788206745743
12	24.64208918202718	23.724473357317976	29.207862389350264	22.42557507130458
13	29.279841061808636	19.70845469488269	25.60598159600434	25.405722647304334
14	23.2524685727882	20.062160447317286	25.29523733988976	31.39013364000476
15	24.919951068541714	27.99581267261325	22.301186211162637	24.783050047682398
16	25.546233150024637	25.923557596504743	24.426255674489756	24.103953578980867
17	23.35980252200809	26.30759774764058	25.742624315964786	24.58997541438655
18	24.226325746308614	25.36398285086519	26.843908765333683	23.565782637492514
19	25.34037684366659	24.850979785601258	25.603516996038024	24.205126374694128
20	25.699472309641802	24.598778836066973	25.17326924106585	24.528479613225375
21	26.609953548783693	24.15790439080494	24.71105558777892	24.52108647263245
22	25.864183637349747	24.778370596289932	25.1251049771306	24.232340789229717
23	24.256801845885974	24.551592294794418	25.82485302618806	25.36675283313155
24	24.821109345146677	25.124407952987227	25.508513479883042	24.545969221983054
25	24.48882869341223	24.94194727960292	25.748141420591214	24.82108260639363
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	51.0
2	187.0
3	323.0
4	323.0
5	323.0
6	994.5
7	1666.0
8	1666.0
9	1666.0
10	1832.5
11	1999.0
12	1999.0
13	1999.0
14	2252.0
15	2505.0
16	2505.0
17	2505.0
18	4394.5
19	6284.0
20	6284.0
21	6284.0
22	10460.0
23	14636.0
24	14636.0
25	14636.0
26	22659.0
27	30682.0
28	30682.0
29	30682.0
30	39469.5
31	48257.0
32	48257.0
33	48257.0
34	58115.5
35	67974.0
36	67974.0
37	67974.0
38	77355.0
39	86736.0
40	86736.0
41	86736.0
42	97876.5
43	109017.0
44	109017.0
45	109017.0
46	119863.5
47	130710.0
48	130710.0
49	130710.0
50	132490.0
51	134270.0
52	134270.0
53	134270.0
54	123369.0
55	112468.0
56	112468.0
57	112468.0
58	102423.0
59	92378.0
60	92378.0
61	92378.0
62	81690.0
63	71002.0
64	71002.0
65	71002.0
66	59362.5
67	47723.0
68	47723.0
69	47723.0
70	35935.5
71	24148.0
72	24148.0
73	24148.0
74	18563.0
75	12978.0
76	12978.0
77	12978.0
78	10377.0
79	7776.0
80	7776.0
81	7776.0
82	5322.5
83	2869.0
84	2869.0
85	2869.0
86	2228.0
87	1587.0
88	1587.0
89	1587.0
90	1103.0
91	619.0
92	619.0
93	619.0
94	423.5
95	228.0
96	228.0
97	228.0
98	341.0
99	454.0
100	454.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03735114034913904
2	0.005548179998811104
3	0.001387044999702776
4	0.0032694632135851155
5	0.008619493926724394
6	0.012879703568668634
7	0.022985317137931716
8	0.03427982642122575
9	0.04745675391840212
10	0.05459012820258784
11	0.052113262131690014
12	0.06330869677214813
13	0.06400221927199952
14	0.0653892642717023
15	0.05766144213050112
16	0.06529018962886639
17	0.061426278558265796
18	0.07688192284066817
19	0.07450413141260626
20	0.07866526641171458
21	0.074801355341114
22	0.07797174391186319
23	0.0819347296252997
24	0.0755939524838013
25	0.07668377355499634
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1009340.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.98023689047106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28132403857492	38.37859732914863
2	14.268661402404428	14.548394768089977
3	4.769104907859199	7.293902938745105
4	1.9936406937742397	4.065450993723753
5	1.0191009822586445	2.5977004745428727
6	0.5918644671507209	1.810403444543771
7	0.3850445181579652	1.3740762524349248
8	0.2637230246816233	1.0755729817392563
9	0.19688668208039806	0.9033596723733802
>10	1.018561798970811	9.485412394234885
>50	0.10360980063113549	3.7524720462882675
>100	0.09696656255514058	10.104185646174582
>500	0.009364979715914048	3.225429971469963
>1k	0.0021461411848969695	1.385041086490663
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2188	0.2167753185249767	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1638	0.1622842649652248	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1276	0.12641924425862444	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1167	0.11562010818950998	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1155	0.11443121247547902	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1133	0.11225157033308895	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1127	0.11165712247607348	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1115	0.11046822676204253	No Hit
GAACTACGACGGTATCTGATCGTCT	1074	0.10640616640577011	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1034	0.1024431806923336	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1018	0.100857986406959	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.907464283591257E-5
2	0.0	0.0	0.0	0.0	9.907464283591257E-5
3	0.0	0.0	0.0	0.0	9.907464283591257E-5
4	0.0	0.0	0.0	0.0	1.9814928567182515E-4
5	0.0	0.0	0.0	0.0	1.9814928567182515E-4
6	0.0	0.0	0.0	0.0	1.9814928567182515E-4
7	0.0	0.0	0.0	0.0	1.9814928567182515E-4
8	0.0	0.0	0.0	0.0	1.9814928567182515E-4
9	0.0	0.0	0.0	0.0	1.9814928567182515E-4
10	0.0	0.0	0.0	0.0	1.9814928567182515E-4
11	0.0	0.0	0.0	0.0	1.9814928567182515E-4
12	0.0	0.0	0.0	0.0	2.972239285077377E-4
13	0.0	0.0	0.0	0.0	2.972239285077377E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCGT	25	0.006035734	18.997225	6
TGCTCGA	45	3.535315E-5	16.88977	10
GCGTTAT	75	5.8345904E-8	15.200793	1
TCGAACG	60	2.5736435E-5	14.245799	3
AACCGCG	75	9.657324E-7	13.934061	7
AGGCCCG	220	0.0	13.818903	10
CACCGTG	55	1.9582108E-4	13.818903	7
GAACGTC	70	7.267463E-6	13.569446	5
CGAACGA	85	2.6925045E-7	13.41313	16
AAACGTA	85	2.6941234E-7	13.412464	18
GTTTAGC	50	0.0014972016	13.302013	14
TTAGGGT	50	0.0015005623	13.298057	4
ACGGTAT	180	0.0	13.194479	9
CAAATCG	65	5.4435826E-5	13.155186	13
CGAACGT	65	5.4562606E-5	13.151924	4
TGGGTAT	80	2.0006992E-6	13.060591	6
GTTGGAC	110	2.75395E-9	12.9507265	3
GTATCAA	1550	0.0	12.749053	1
GGTATCA	500	0.0	12.730663	1
AATCGCT	75	1.4750665E-5	12.668584	15
>>END_MODULE