FastQCFastQC Report
Thu 2 Feb 2017
SRR4064009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009340
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12750.12632016961578854No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11660.11552103354667406No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11170.11066637604771434No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10940.10838765926248835No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10670.10571264390591872No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10660.10561356926308281No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10310.10214595676382586No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG10210.10115521033546675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19750.024.2852381
GTCCTAC5050.021.3480241
GTATGAC1057.7494406E-820.9507313
GTATTAC1301.6225385E-920.309111
GATTTCG2400.020.16607541
GATATAC2850.019.2995931
TCCTACA5050.019.1668052
GTATAGA1759.094947E-1218.858461
GGTATCA8900.018.5406191
CGTCTTA951.5977681E-518.52485715
GTAGGAC6200.018.450163
AATCACG2550.018.11891434
AACGCAG26250.018.0176286
ATCACGG2600.017.77135335
GCGAGAA3600.017.7234620
TAGGACG5250.017.5986124
TCGACGC750.001291352617.59861214
GCACACG4450.017.3020067
GTATAAT1154.2686315E-617.2185941
TGTAGGA6800.017.1457062
Produced by FastQC (version 0.11.5)