##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064009_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1009340
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24661263796144	32.0	32.0	32.0	32.0	32.0
2	31.233037430400064	32.0	32.0	32.0	32.0	32.0
3	31.359602314383658	32.0	32.0	32.0	32.0	32.0
4	31.46645332593576	32.0	32.0	32.0	32.0	32.0
5	31.386230606138664	32.0	32.0	32.0	32.0	32.0
6	34.94644916480076	36.0	36.0	36.0	36.0	36.0
7	34.96958705688866	36.0	36.0	36.0	36.0	36.0
8	34.899429330057266	36.0	36.0	36.0	36.0	36.0
9	35.04639764598649	36.0	36.0	36.0	36.0	36.0
10	34.871907385023874	36.0	36.0	36.0	32.0	36.0
11	35.04120811619474	36.0	36.0	36.0	36.0	36.0
12	34.93547565736026	36.0	36.0	36.0	32.0	36.0
13	34.99492935977966	36.0	36.0	36.0	36.0	36.0
14	34.93511799789962	36.0	36.0	36.0	32.0	36.0
15	34.88400043592843	36.0	36.0	36.0	32.0	36.0
16	34.908490696891036	36.0	36.0	36.0	32.0	36.0
17	34.858666059008854	36.0	36.0	36.0	32.0	36.0
18	34.86766302732479	36.0	36.0	36.0	32.0	36.0
19	34.86797610319615	36.0	36.0	36.0	32.0	36.0
20	34.86519507797174	36.0	36.0	36.0	32.0	36.0
21	34.84608159787584	36.0	36.0	36.0	32.0	36.0
22	34.83510313670319	36.0	36.0	36.0	32.0	36.0
23	34.7755731468088	36.0	36.0	36.0	32.0	36.0
24	34.748916123407376	36.0	36.0	36.0	32.0	36.0
25	34.717792815106904	36.0	36.0	36.0	32.0	36.0
26	34.65862345691244	36.0	36.0	36.0	32.0	36.0
27	34.66311550121862	36.0	36.0	36.0	32.0	36.0
28	34.63086868646838	36.0	36.0	36.0	32.0	36.0
29	34.59632928448293	36.0	36.0	36.0	32.0	36.0
30	34.579008064675925	36.0	36.0	36.0	32.0	36.0
31	34.572022311609565	36.0	36.0	36.0	32.0	36.0
32	34.52734559216914	36.0	36.0	36.0	32.0	36.0
33	34.51167396516536	36.0	36.0	36.0	32.0	36.0
34	34.50371827134563	36.0	36.0	36.0	32.0	36.0
35	34.45580082033804	36.0	36.0	36.0	32.0	36.0
36	34.43479006083183	36.0	36.0	36.0	32.0	36.0
37	34.424672558305424	36.0	36.0	36.0	32.0	36.0
38	34.37369568232707	36.0	36.0	36.0	32.0	36.0
39	34.364843362989674	36.0	36.0	36.0	32.0	36.0
40	34.35354191848138	36.0	36.0	36.0	32.0	36.0
41	34.33491984861394	36.0	36.0	36.0	32.0	36.0
42	34.26706659797492	36.0	36.0	36.0	32.0	36.0
43	34.26603523094299	36.0	36.0	36.0	32.0	36.0
44	34.191882814512454	36.0	36.0	36.0	32.0	36.0
45	34.1530604157172	36.0	36.0	36.0	32.0	36.0
46	34.12599421404086	36.0	36.0	36.0	32.0	36.0
47	34.0965343689936	36.0	36.0	36.0	32.0	36.0
48	34.05369647492421	36.0	36.0	36.0	32.0	36.0
49	34.046457090772186	36.0	36.0	36.0	32.0	36.0
50	33.426139853765825	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	1.0
19	4.0
20	6.0
21	35.0
22	112.0
23	328.0
24	799.0
25	1902.0
26	3933.0
27	7509.0
28	12531.0
29	19712.0
30	28734.0
31	41896.0
32	60960.0
33	99038.0
34	199010.0
35	532829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.40495690893706	18.30859899054553	12.028936109498265	26.257507991019146
2	15.685507161660226	20.271406958853024	37.64367161283739	26.399414266649362
3	18.326008385711003	24.207597866320036	28.95814674201653	28.50824700595243
4	11.906592426734301	15.911486714090394	36.296490776150755	35.88543008302455
5	13.908890958448094	37.4048388055561	33.362890601779384	15.323379634216419
6	34.0821725087681	36.54506905502606	16.315810331503755	13.056948104702082
7	29.98400932096087	30.780174728386328	20.512751922547253	18.72306402810555
8	27.98987457150217	33.5955178631581	18.975766342362334	19.43884122297739
9	27.186153190730483	13.786373138616707	18.418356731693304	40.6091169389595
10	15.405436466890974	27.04125104524577	31.669460593739224	25.88385189412403
11	37.88816245087131	20.904473125481132	22.33778740343928	18.869577020208276
12	24.685090623755375	23.776871573249	29.08827370018765	22.44976410280798
13	29.555341773857492	19.66241205860877	25.49911476543513	25.283131402098608
14	23.369399913012202	19.883349168810813	25.258164517896404	31.48908640028058
15	25.028831635140115	28.049614191109157	22.145569043344548	24.77598513040618
16	25.661277634559028	25.934923424753354	24.204376351138475	24.19942258954915
17	23.46781064854261	26.37119305684903	25.50468623060614	24.65631006400222
18	24.416150815981993	25.38579818811575	26.568754111613973	23.629296884288284
19	25.503596409534946	24.90795965680544	25.414132007054114	24.174311926605505
20	25.89474309945113	24.580319812947074	25.03279370677869	24.492143380823112
21	26.809301127469435	24.217508470881963	24.504230487249092	24.46895991439951
22	25.980765629783452	24.72707385724717	25.040843561974718	24.25131695099466
23	24.333942645405145	24.518618694486964	25.705836020746332	25.44160263936156
24	24.950933137297056	25.083495403418283	25.48643317345941	24.47913828582525
25	24.787827027755373	24.748691690824895	25.562706698978914	24.900774582440814
26	24.099788861686076	25.678619942117788	26.27676259869552	23.944828597500624
27	24.783658943054792	25.016001410898397	25.50436645311636	24.69597319293045
28	23.964002524539456	25.128877342852867	26.03763228319229	24.869487849415385
29	24.323405857408947	25.011889192723814	25.70720881385487	24.957496136012363
30	24.105105572228126	25.413921801945687	26.0103694705982	24.470603155227984
31	25.106934042230648	24.96683236447838	24.900546634339577	25.025686958951397
32	24.653354007420873	25.22175931200864	24.716664272303493	25.408222408266994
33	24.193524416451417	24.89242533679316	25.75232909177558	25.161721154979844
34	24.936738960279794	25.101009600618244	25.925236052352595	24.037015386749363
35	25.37092355630855	24.874938695438942	25.58780547010071	24.1663322781518
36	24.015650900501452	25.501872143358916	25.451538783934147	25.03093817220548
37	25.26924441150652	25.147477573699156	24.967551961446777	24.615726053347547
38	24.461438159764263	25.17710961078491	25.45721527214935	24.904236957301475
39	25.304267874623008	24.707918692807347	25.041018202570477	24.946795229999168
40	25.497117855819475	25.28608426680194	25.375055235317312	23.841742642061273
41	24.346526001240466	25.514268333039396	26.01035571258439	24.128849953135745
42	25.663312619881744	25.83887497424029	25.02387727279933	23.47393513307864
43	24.64828083635845	24.908255789990964	25.64627157871376	24.79719179493683
44	24.437374855225887	25.470930745216386	25.000916448617943	25.09077795093978
45	24.505611654486945	25.87721731686825	24.946399980977443	24.67077104766736
46	24.439975032942645	25.097936254743246	24.98102701792278	25.48106169439133
47	24.790900065984626	25.137071669609224	25.30986024337033	24.76216802103582
48	25.42404619329373	25.669160745211645	24.359472280457016	24.547320781037605
49	24.79015907487695	25.734443237930677	24.81443245856682	24.660965228625553
50	24.86243295030545	26.03787081676287	24.44779981888904	24.651896414042636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	9.5
2	10.0
3	39.0
4	68.0
5	90.5
6	113.0
7	111.5
8	110.0
9	152.5
10	195.0
11	325.5
12	456.0
13	862.0
14	1268.0
15	1757.5
16	2247.0
17	2372.5
18	2498.0
19	2528.5
20	2559.0
21	2878.0
22	3197.0
23	3811.0
24	4425.0
25	5519.5
26	6614.0
27	8310.5
28	10007.0
29	11750.0
30	13493.0
31	15566.5
32	17640.0
33	20799.5
34	23959.0
35	27980.0
36	32001.0
37	37115.5
38	42230.0
39	45316.0
40	48402.0
41	52241.0
42	56080.0
43	56628.5
44	57177.0
45	62404.0
46	67631.0
47	70695.5
48	73760.0
49	75717.5
50	77675.0
51	74490.0
52	71305.0
53	70122.0
54	68939.0
55	68928.5
56	68918.0
57	66413.5
58	63909.0
59	57880.5
60	51852.0
61	45676.5
62	39501.0
63	34789.5
64	30078.0
65	25819.5
66	21561.0
67	19241.0
68	16921.0
69	15246.0
70	13571.0
71	10152.0
72	6733.0
73	5677.5
74	4622.0
75	3595.5
76	2569.0
77	2213.0
78	1857.0
79	1567.5
80	1278.0
81	1042.0
82	806.0
83	666.0
84	526.0
85	385.5
86	245.0
87	184.0
88	123.0
89	95.5
90	68.0
91	59.0
92	50.0
93	36.0
94	22.0
95	29.0
96	36.0
97	23.5
98	11.0
99	13.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00812412071254483
2	6.93522499851388E-4
3	3.9629857134365035E-4
4	0.0
5	0.0
6	0.0
7	1.9814928567182517E-4
8	0.0
9	9.907464283591258E-4
10	7.925971426873007E-4
11	9.907464283591259E-5
12	1.9814928567182517E-4
13	2.972239285077377E-4
14	2.972239285077377E-4
15	0.003170388570749203
16	5.944478570154754E-4
17	0.0
18	3.9629857134365035E-4
19	0.0
20	0.0
21	0.0
22	9.907464283591259E-5
23	4.953732141795629E-4
24	2.972239285077377E-4
25	0.0021796421423900766
26	0.004854657498959716
27	0.005350030713139279
28	0.004656508213287891
29	0.0019814928567182515
30	0.00307131392791329
31	0.007232448927021619
32	0.0024768660708978144
33	0.004062060356272415
34	0.0029722392850773773
35	0.00346761249925694
36	0.006836150355677968
37	0.0033685378564210274
38	0.0077278221412011815
39	0.003170388570749203
40	0.0021796421423900766
41	0.004161134999108328
42	0.0011888957140309508
43	0.0011888957140309508
44	8.916717855232131E-4
45	0.0011888957140309508
46	9.907464283591258E-4
47	0.001387044999702776
48	0.0015851942853746014
49	3.9629857134365035E-4
50	0.001387044999702776
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1009340.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.91577858192838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4250488478139	41.96941060639609
2	13.790444569135765	15.146260010100326
3	4.583811453115678	7.551707244618239
4	1.9110360102608182	4.197841216062996
5	0.9680980727290496	2.658192970379004
6	0.5216720239593791	1.7188815216083817
7	0.35576022813106134	1.3675794941411186
8	0.24175062033142455	1.0620738830531475
9	0.17435126080815774	0.8617171710618755
>10	0.8618251346890325	8.656047964753872
>50	0.08092568118685123	3.122750033452108
>100	0.07654848044083255	8.235695263232861
>500	0.0072730144984507515	2.5694609826380064
>1k	0.0014546028996901502	0.8823816385020037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1275	0.12632016961578854	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1166	0.11552103354667406	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1117	0.11066637604771434	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1094	0.10838765926248835	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1067	0.10571264390591872	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1066	0.10561356926308281	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1031	0.10214595676382586	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1021	0.10115521033546675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.907464283591257E-5
2	0.0	0.0	0.0	0.0	9.907464283591257E-5
3	0.0	0.0	0.0	0.0	9.907464283591257E-5
4	0.0	0.0	0.0	0.0	9.907464283591257E-5
5	0.0	0.0	0.0	0.0	9.907464283591257E-5
6	0.0	0.0	0.0	0.0	9.907464283591257E-5
7	0.0	0.0	0.0	0.0	9.907464283591257E-5
8	0.0	0.0	0.0	0.0	9.907464283591257E-5
9	0.0	0.0	0.0	0.0	9.907464283591257E-5
10	0.0	0.0	0.0	0.0	9.907464283591257E-5
11	0.0	0.0	0.0	0.0	9.907464283591257E-5
12	0.0	0.0	0.0	0.0	4.953732141795629E-4
13	0.0	0.0	0.0	0.0	4.953732141795629E-4
14	0.0	0.0	0.0	0.0	4.953732141795629E-4
15	0.0	0.0	0.0	0.0	4.953732141795629E-4
16	0.0	0.0	0.0	0.0	6.935224998513881E-4
17	0.0	0.0	0.0	0.0	6.935224998513881E-4
18	0.0	0.0	0.0	0.0	7.925971426873006E-4
19	0.0	0.0	0.0	0.0	7.925971426873006E-4
20	0.0	0.0	0.0	0.0	8.916717855232132E-4
21	0.0	0.0	0.0	0.0	8.916717855232132E-4
22	0.0	0.0	0.0	0.0	9.907464283591258E-4
23	0.0	0.0	0.0	9.907464283591257E-5	9.907464283591258E-4
24	0.0	0.0	0.0	9.907464283591257E-5	0.0010898210711950383
25	0.0	0.0	0.0	9.907464283591257E-5	0.0010898210711950383
26	0.0	0.0	0.0	9.907464283591257E-5	0.0010898210711950383
27	0.0	0.0	0.0	9.907464283591257E-5	0.0010898210711950383
28	0.0	0.0	0.0	3.962985713436503E-4	0.0010898210711950383
29	0.0	0.0	0.0	9.907464283591258E-4	0.0010898210711950383
30	0.0	0.0	0.0	0.0014861196425386886	0.0010898210711950383
31	0.0	0.0	0.0	0.004260209641944241	0.0010898210711950383
32	0.0	0.0	0.0	0.00614262785582658	0.0010898210711950383
33	0.0	0.0	0.0	0.007925971426873006	0.0010898210711950383
34	0.0	0.0	0.0	0.01079913606911447	0.0012879703568668636
35	0.0	0.0	0.0	0.013969524639863673	0.0012879703568668636
36	0.0	0.0	0.0	0.01922048071016704	0.0012879703568668636
37	0.0	0.0	0.0	0.029524243565101947	0.0012879703568668636
38	0.0	0.0	0.0	0.043691917490637444	0.0012879703568668636
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1975	0.0	24.285238	1
GTCCTAC	505	0.0	21.348024	1
GTATGAC	105	7.7494406E-8	20.950731	3
GTATTAC	130	1.6225385E-9	20.30911	1
GATTTCG	240	0.0	20.166075	41
GATATAC	285	0.0	19.299593	1
TCCTACA	505	0.0	19.166805	2
GTATAGA	175	9.094947E-12	18.85846	1
GGTATCA	890	0.0	18.540619	1
CGTCTTA	95	1.5977681E-5	18.524857	15
GTAGGAC	620	0.0	18.45016	3
AATCACG	255	0.0	18.118914	34
AACGCAG	2625	0.0	18.017628	6
ATCACGG	260	0.0	17.771353	35
GCGAGAA	360	0.0	17.72346	20
TAGGACG	525	0.0	17.598612	4
TCGACGC	75	0.0012913526	17.598612	14
GCACACG	445	0.0	17.302006	7
GTATAAT	115	4.2686315E-6	17.218594	1
TGTAGGA	680	0.0	17.145706	2
>>END_MODULE