FastQCFastQC Report
Thu 2 Feb 2017
SRR4064008_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064008_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299907
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64090.2786634416087259No Hit
TATCAACGCAGAGTACTTTTTTTTT47340.20583440982613643No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34200.14870166489340655No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG25250.1097870479110677No Hit
GGTATCAACGCAGAGTACTTTTTTT24620.10704780671566284No Hit
GTACATGGGGTGGTATCAACGCAAA24430.10622168635514392No Hit
GAATAGGACCGCGGTTCTATTTTGT23530.10230848464742269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1600.014.25135316
CGAATTG400.00527907914.25042314
CGTATTA400.00529128314.2454642
AATCGTA1001.0077201E-813.30039613
ACGAACG1800.012.667870515
CGGTCCA3300.012.09179510
TACGCTG550.003072532612.0891665
ATTTAGA2000.011.8725121
GATAACG2250.011.82283111
GTTTACG658.021054E-411.6931649
GCGTAAC1950.011.6929111
CGCATCG3300.011.51549413
TCGCGTA1900.011.5008439
TAACGAA2000.011.40033913
CAAGACG5750.011.3976114
GCATCGC3400.011.17680414
AAGACGG5950.011.1748575
GAAACGC600.005872954611.08438616
ATAACGA2250.010.97810512
CATCGCC3550.010.97287415
Produced by FastQC (version 0.11.5)