##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064008_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2299907
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210111104492487	32.0	32.0	32.0	32.0	32.0
2	30.751213418629536	32.0	32.0	32.0	32.0	32.0
3	30.77361649840624	32.0	32.0	32.0	32.0	32.0
4	30.801875032338263	32.0	32.0	32.0	32.0	32.0
5	30.695628997172495	32.0	32.0	32.0	32.0	32.0
6	34.35189814196835	36.0	36.0	36.0	32.0	36.0
7	34.28097353501685	36.0	36.0	36.0	32.0	36.0
8	34.227417456444975	36.0	36.0	36.0	32.0	36.0
9	34.39410550078764	36.0	36.0	36.0	32.0	36.0
10	34.044256137313376	36.0	36.0	36.0	32.0	36.0
11	34.38949966237765	36.0	36.0	36.0	32.0	36.0
12	34.16277310343418	36.0	36.0	36.0	32.0	36.0
13	34.26666599997304	36.0	36.0	36.0	32.0	36.0
14	34.153831872332226	36.0	36.0	36.0	32.0	36.0
15	34.08054064794794	36.0	36.0	36.0	32.0	36.0
16	34.077504003422746	36.0	36.0	36.0	32.0	36.0
17	33.99945215176092	36.0	36.0	36.0	32.0	36.0
18	33.99803905114424	36.0	36.0	36.0	32.0	36.0
19	34.017199825906005	36.0	36.0	36.0	32.0	36.0
20	33.990538747871106	36.0	36.0	36.0	32.0	36.0
21	33.97422504475181	36.0	36.0	36.0	32.0	36.0
22	33.95425380243636	36.0	36.0	36.0	32.0	36.0
23	33.89165083631642	36.0	36.0	36.0	32.0	36.0
24	33.8757410625734	36.0	36.0	36.0	32.0	36.0
25	33.46709062583835	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	26.0
5	30.0
6	93.0
7	27.0
8	148.0
9	144.0
10	217.0
11	80.0
12	155.0
13	105.0
14	247.0
15	400.0
16	678.0
17	855.0
18	1190.0
19	1716.0
20	2568.0
21	3865.0
22	6003.0
23	9358.0
24	13884.0
25	19720.0
26	28043.0
27	37092.0
28	49507.0
29	66661.0
30	87329.0
31	117955.0
32	166218.0
33	232924.0
34	485051.0
35	967607.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59192617962876	17.297843484040353	11.293413704998638	25.816816631332244
2	16.396424366072004	19.984537705214567	38.07716189492249	25.54187603379094
3	18.64472737929493	24.291500968318967	28.825903467251514	28.237868185134584
4	12.120213356662788	15.563496304696342	36.64540260047456	35.670887738166314
5	14.107933255613018	37.20539079244718	33.89582723652095	14.790848715418845
6	33.42520486278411	36.29106605183189	17.252177346550774	13.031551738833217
7	29.892183839361707	30.75633095399319	20.959242438277847	18.392242768367257
8	27.952109289503397	33.30088540225866	19.554402044654616	19.19260326358333
9	27.648687847527036	13.844137358607448	18.63232150454466	39.87485328932085
10	15.600292879911839	26.85076219691258	32.01657738585971	25.53236753731587
11	36.987454543002464	20.989506225743398	23.13146126709246	18.89157796416168
12	24.648208432207408	23.848427021142736	29.46904295115947	22.034321595490386
13	29.596188962907306	19.663353749189103	25.5087829368289	25.231674351074695
14	23.224345076253492	20.05507498734946	25.659427033908667	31.06115290248838
15	24.85561229306604	28.072885826793918	22.431247522839907	24.640254357300133
16	25.161780721368192	25.84627861918691	24.75378829905405	24.238152360390842
17	23.45537369149242	26.269263233003542	25.71940724497698	24.555955830527058
18	24.05269850055743	25.249102066357015	27.238567936175563	23.459631496909992
19	25.414335070970438	24.83621537417216	25.72467299642319	24.024776558434212
20	25.529290996145825	24.591530038593977	25.396698994398236	24.482479970861963
21	26.65885742905219	24.223271673136413	24.631385563846827	24.48648533396457
22	25.674534293386575	24.888873997146295	25.202964056313416	24.233627653153718
23	24.131810115311875	24.710197211243802	25.818971932276796	25.339020741167534
24	24.558355166995423	25.18701668409443	25.87337911983906	24.381249029071082
25	24.39813600026805	24.940547912181923	25.900875909296023	24.760440178254004
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	215.0
1	215.0
2	618.5
3	1022.0
4	1022.0
5	1022.0
6	2965.0
7	4908.0
8	4908.0
9	4908.0
10	4877.0
11	4846.0
12	4846.0
13	4846.0
14	5478.5
15	6111.0
16	6111.0
17	6111.0
18	10660.5
19	15210.0
20	15210.0
21	15210.0
22	25479.0
23	35748.0
24	35748.0
25	35748.0
26	55300.5
27	74853.0
28	74853.0
29	74853.0
30	94954.0
31	115055.0
32	115055.0
33	115055.0
34	137095.5
35	159136.0
36	159136.0
37	159136.0
38	179055.0
39	198974.0
40	198974.0
41	198974.0
42	223805.5
43	248637.0
44	248637.0
45	248637.0
46	273721.5
47	298806.0
48	298806.0
49	298806.0
50	300784.0
51	302762.0
52	302762.0
53	302762.0
54	276348.0
55	249934.0
56	249934.0
57	249934.0
58	229124.5
59	208315.0
60	208315.0
61	208315.0
62	183542.5
63	158770.0
64	158770.0
65	158770.0
66	131038.0
67	103306.0
68	103306.0
69	103306.0
70	78335.0
71	53364.0
72	53364.0
73	53364.0
74	41265.0
75	29166.0
76	29166.0
77	29166.0
78	23198.5
79	17231.0
80	17231.0
81	17231.0
82	11927.0
83	6623.0
84	6623.0
85	6623.0
86	5074.0
87	3525.0
88	3525.0
89	3525.0
90	2516.0
91	1507.0
92	1507.0
93	1507.0
94	1067.5
95	628.0
96	628.0
97	628.0
98	941.5
99	1255.0
100	1255.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04004509747568054
2	0.005174122257986954
3	0.0019566008538606126
4	0.004174081821569307
5	0.009043843946733498
6	0.01387012605292301
7	0.02304441005658055
8	0.036479735919756756
9	0.05065422210550253
10	0.0588719456917171
11	0.05678490478093245
12	0.06648094901228616
13	0.06652442903126084
14	0.06895931009384293
15	0.06121986671634984
16	0.06869842997999484
17	0.06465478821534958
18	0.08056847516008256
19	0.07682919352826005
20	0.08174243567239893
21	0.07713355366108282
22	0.08000323491341171
23	0.08543823728524676
24	0.08043803510315851
25	0.07991627487546236
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2299907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.16384477259013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.13828173695859	31.417400320198226
2	15.279431649346012	13.495968951502363
3	5.740412215310804	7.605560220229999
4	2.6210184141916737	4.630170015618456
5	1.4582324022827238	3.2200574728387705
6	0.8551032336827736	2.2658787886143528
7	0.5215548737142206	1.6123707938171885
8	0.38308420230668855	1.3534776996403286
9	0.27905210202412595	1.1091612345532635
>10	1.4916980102916044	11.993802170995943
>50	0.11750265569429644	3.5807137612940534
>100	0.09529827281792312	8.964779520079011
>500	0.013018319091658237	4.038467524684415
>1k	0.0062132886573823265	4.4330412075985475
>5k	9.862362948225913E-5	0.2791503183349947
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6409	0.2786634416087259	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4734	0.20583440982613643	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3420	0.14870166489340655	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2525	0.1097870479110677	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2462	0.10704780671566284	No Hit
GTACATGGGGTGGTATCAACGCAAA	2443	0.10622168635514392	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2353	0.10230848464742269	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.3480018974680284E-5	0.0
11	0.0	0.0	0.0	4.3480018974680284E-5	0.0
12	0.0	0.0	0.0	4.3480018974680284E-5	0.0
13	0.0	0.0	0.0	4.3480018974680284E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	160	0.0	14.251353	16
CGAATTG	40	0.005279079	14.250423	14
CGTATTA	40	0.005291283	14.245464	2
AATCGTA	100	1.0077201E-8	13.300396	13
ACGAACG	180	0.0	12.6678705	15
CGGTCCA	330	0.0	12.091795	10
TACGCTG	55	0.0030725326	12.089166	5
ATTTAGA	200	0.0	11.872512	1
GATAACG	225	0.0	11.822831	11
GTTTACG	65	8.021054E-4	11.693164	9
GCGTAAC	195	0.0	11.69291	11
CGCATCG	330	0.0	11.515494	13
TCGCGTA	190	0.0	11.500843	9
TAACGAA	200	0.0	11.400339	13
CAAGACG	575	0.0	11.397611	4
GCATCGC	340	0.0	11.176804	14
AAGACGG	595	0.0	11.174857	5
GAAACGC	60	0.0058729546	11.084386	16
ATAACGA	225	0.0	10.978105	12
CATCGCC	355	0.0	10.972874	15
>>END_MODULE