FastQCFastQC Report
Thu 2 Feb 2017
SRR4064008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299907
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24140.1049607658048782No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23770.10335200510281503No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23390.10169976438177718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG7550.020.3963344
TGTAGGA14050.019.8855612
GTATCAA52050.019.4435631
ATACCGT6300.018.8562036
GTAGGAC14750.018.6435433
GGACGTG8300.018.5532916
AATCACG4900.018.40924534
AGGACGT9000.018.3324185
CGTCGTA6150.018.24299210
TACCGTC6800.018.1167437
ACCGTCG6400.017.874118
AGTATCG750.001291783117.5991218
GTCCTAA6950.017.0941581
CCGTCGT6700.017.0737749
CGCAATA7350.017.06222736
CTGTAGG15750.017.04191
TTAGGAC7650.016.9668433
TCACGGA5900.016.78064736
AACGCAG60700.016.7800526
ATTTCGT4500.016.62211642
Produced by FastQC (version 0.11.5)