##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064008_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2299907
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300423017104606	32.0	32.0	32.0	32.0	32.0
2	31.405829018303784	32.0	32.0	32.0	32.0	32.0
3	31.481848613878736	32.0	32.0	32.0	32.0	32.0
4	31.56162227429196	32.0	32.0	32.0	32.0	32.0
5	31.51376468700691	32.0	32.0	32.0	32.0	32.0
6	35.09465252290636	36.0	36.0	36.0	36.0	36.0
7	35.11166451513039	36.0	36.0	36.0	36.0	36.0
8	35.062870803036816	36.0	36.0	36.0	36.0	36.0
9	35.16808288335137	36.0	36.0	36.0	36.0	36.0
10	35.02434315822335	36.0	36.0	36.0	36.0	36.0
11	35.168002880116454	36.0	36.0	36.0	36.0	36.0
12	35.071768988919985	36.0	36.0	36.0	36.0	36.0
13	35.1146115908165	36.0	36.0	36.0	36.0	36.0
14	35.061767280155244	36.0	36.0	36.0	36.0	36.0
15	35.02790982417985	36.0	36.0	36.0	36.0	36.0
16	35.04368307066329	36.0	36.0	36.0	36.0	36.0
17	35.007412038834616	36.0	36.0	36.0	36.0	36.0
18	35.002822288031645	36.0	36.0	36.0	36.0	36.0
19	35.00399755294453	36.0	36.0	36.0	36.0	36.0
20	34.98998002962728	36.0	36.0	36.0	36.0	36.0
21	34.984900693810665	36.0	36.0	36.0	36.0	36.0
22	34.96800348883672	36.0	36.0	36.0	36.0	36.0
23	34.92236729572109	36.0	36.0	36.0	32.0	36.0
24	34.89276740320369	36.0	36.0	36.0	32.0	36.0
25	34.86353622124721	36.0	36.0	36.0	32.0	36.0
26	34.80838225197802	36.0	36.0	36.0	32.0	36.0
27	34.80533865064979	36.0	36.0	36.0	32.0	36.0
28	34.77468436767226	36.0	36.0	36.0	32.0	36.0
29	34.74417791675924	36.0	36.0	36.0	32.0	36.0
30	34.72783073402533	36.0	36.0	36.0	32.0	36.0
31	34.72344620891192	36.0	36.0	36.0	32.0	36.0
32	34.67696737302856	36.0	36.0	36.0	32.0	36.0
33	34.65511214149094	36.0	36.0	36.0	32.0	36.0
34	34.63954890349914	36.0	36.0	36.0	32.0	36.0
35	34.60046558404318	36.0	36.0	36.0	32.0	36.0
36	34.571424844569805	36.0	36.0	36.0	32.0	36.0
37	34.55453503119909	36.0	36.0	36.0	32.0	36.0
38	34.51336684483329	36.0	36.0	36.0	32.0	36.0
39	34.4988423444948	36.0	36.0	36.0	32.0	36.0
40	34.48272256226013	36.0	36.0	36.0	32.0	36.0
41	34.45911943395972	36.0	36.0	36.0	32.0	36.0
42	34.39159757329318	36.0	36.0	36.0	32.0	36.0
43	34.38871267403421	36.0	36.0	36.0	32.0	36.0
44	34.311408243898555	36.0	36.0	36.0	32.0	36.0
45	34.27465501865945	36.0	36.0	36.0	32.0	36.0
46	34.233635099158356	36.0	36.0	36.0	32.0	36.0
47	34.204543488062775	36.0	36.0	36.0	32.0	36.0
48	34.14682550207465	36.0	36.0	36.0	32.0	36.0
49	34.110508816226044	36.0	36.0	36.0	32.0	36.0
50	33.52398770906824	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	6.0
19	7.0
20	19.0
21	96.0
22	281.0
23	798.0
24	1994.0
25	4539.0
26	9184.0
27	16572.0
28	27403.0
29	41307.0
30	59426.0
31	83330.0
32	119956.0
33	190737.0
34	396394.0
35	1347855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58158085380055	18.212292591970932	11.848098051305957	26.358028502922565
2	15.643345922297424	20.66865574038501	37.686280000243485	26.00171833707408
3	17.926953345797646	24.822296621592244	29.154658898212965	28.09609113439715
4	11.805303431834417	16.200437669870997	36.71091918064513	35.283339717649454
5	13.693727615942732	37.48851583998831	33.76232169387719	15.055434850191768
6	33.52973326219978	36.527951879626016	16.99496369188386	12.947351166290346
7	29.652211873006983	30.981918391447305	20.981074878712434	18.384794856833278
8	27.880127326887568	33.47744060955508	19.441786124395467	19.200645939161888
9	27.636458503179522	13.640654383390402	18.632621338116202	40.090265775313874
10	15.446186944755572	27.078492517896525	32.06216998392965	25.41315055341825
11	37.1456920413269	20.904941332384887	23.09611784133786	18.853248784950342
12	24.572395567471016	23.89115033397017	29.42011543080456	22.11633866775425
13	29.64259641644198	19.67113252660015	25.513056476975233	25.173214579982634
14	23.23853517364203	20.005574145703473	25.69168104407836	31.064209636576134
15	24.851573404528242	28.11743680944782	22.43904878271494	24.591941003309
16	25.150159877767642	25.86489219881716	24.713963585957888	24.270984337457314
17	23.48690620968587	26.27827994784137	25.665733440526072	24.56908040194669
18	24.140908673895094	25.180257758920927	27.222823938943456	23.45600962824052
19	25.485998123401565	24.748924521263042	25.803141519696894	23.9619358356385
20	25.646540947774525	24.45513769553627	25.449053499445405	24.449267857243793
21	26.57568327762818	24.232849415215483	24.792219859324746	24.399247447831588
22	25.65240738204404	24.752065846197997	25.401614414508426	24.193912357249538
23	24.139739221630307	24.47562572721059	26.050313841915507	25.334321209243598
24	24.62890697184002	24.998967344161365	25.963837479537112	24.40828820446151
25	24.502054914838	24.731591424143815	26.103331866572283	24.663021794445903
26	24.048991028660282	25.59901694431786	26.44505880593173	23.906933221090128
27	24.69998047628303	25.264298513718998	25.46701470192415	24.568706308073818
28	23.99693189911792	25.140773477972374	26.172605024623945	24.68968959828576
29	24.090185521843505	24.952855354531646	25.946534471790827	25.010424651834022
30	23.933743774999467	25.30790734320695	26.288862635386835	24.469486246406753
31	24.920261303761926	24.933871780611288	25.313182290378002	24.83268462524878
32	24.438835297621768	25.36656209303792	25.271467487602262	24.923135121738053
33	24.006981534328702	24.93650482372017	25.963820033298823	25.0926936086523
34	24.635940007661446	25.098366094493198	26.20305788217983	24.062636015665525
35	25.161544759671056	24.93639738989063	25.96630094350575	23.935756906932564
36	23.97189210813537	25.616403807436587	25.712807267003228	24.698896817424806
37	24.942744996410557	25.347476018556403	25.296732453575288	24.41304653145775
38	24.358469833199763	25.372603434278517	25.66033699222115	24.60858974030057
39	25.11520597062172	24.9674970888697	25.107727039900098	24.809569900608487
40	25.404755537873204	25.174392187299578	25.684643965136782	23.736208309690436
41	24.25900118921575	25.454086923627127	26.134961855959233	24.15195003119789
42	25.31423706527713	25.86135827634426	25.466420506327353	23.35798415205126
43	24.371973461027896	25.077145899560104	25.833582261204253	24.717298378207744
44	24.093935948414362	25.651983920795352	25.28944090196599	24.9646392288243
45	24.26230677185513	25.83000271319447	25.348975943009002	24.558714571941394
46	24.219948771262516	25.202086542738517	25.382704583411055	25.19526010258792
47	24.764103667855878	25.159304213979638	25.663599068632887	24.412993049531597
48	25.034034843157094	25.75047318323942	24.71670966224304	24.498782311360443
49	24.417115708124573	25.79330657563658	25.08679728979403	24.702780426444814
50	24.301306512037232	26.23894282444527	24.79942292239889	24.660327741118603
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	45.0
2	55.0
3	151.5
4	248.0
5	289.0
6	330.0
7	339.5
8	349.0
9	497.5
10	646.0
11	1019.5
12	1393.0
13	2603.5
14	3814.0
15	5250.0
16	6686.0
17	7367.5
18	8049.0
19	8367.5
20	8686.0
21	9584.5
22	10483.0
23	11500.0
24	12517.0
25	14290.5
26	16064.0
27	20199.0
28	24334.0
29	29570.0
30	34806.0
31	39286.5
32	43767.0
33	51935.0
34	60103.0
35	69049.5
36	77996.0
37	88482.0
38	98968.0
39	104600.0
40	110232.0
41	116746.0
42	123260.0
43	124452.5
44	125645.0
45	136749.5
46	147854.0
47	155051.0
48	162248.0
49	168134.5
50	174021.0
51	167188.0
52	160355.0
53	156975.0
54	153595.0
55	154323.0
56	155051.0
57	149823.5
58	144596.0
59	131766.5
60	118937.0
61	104722.0
62	90507.0
63	78938.0
64	67369.0
65	57582.5
66	47796.0
67	41507.5
68	35219.0
69	32093.5
70	28968.0
71	22203.5
72	15439.0
73	13343.0
74	11247.0
75	8780.0
76	6313.0
77	5510.5
78	4708.0
79	3896.5
80	3085.0
81	2493.5
82	1902.0
83	1523.5
84	1145.0
85	827.0
86	509.0
87	374.0
88	239.0
89	184.0
90	129.0
91	100.5
92	72.0
93	57.0
94	42.0
95	41.0
96	40.0
97	34.5
98	29.0
99	27.5
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0092612440416069
2	3.9132017077212253E-4
3	3.0436013282276195E-4
4	0.0
5	0.0
6	3.478401517974422E-4
7	4.348001897468028E-5
8	0.0
9	0.0013913606071897689
10	8.261203605189253E-4
11	4.348001897468028E-5
12	4.348001897468028E-5
13	6.087202656455239E-4
14	1.3044005692404084E-4
15	0.005087162220037592
16	0.0010870004743670071
17	0.0
18	2.608801138480817E-4
19	4.348001897468028E-5
20	6.087202656455239E-4
21	0.0
22	8.696003794936055E-5
23	0.0010000404364176466
24	5.217602276961634E-4
25	0.0023914010436074155
26	0.006043722637480559
27	0.006087202656455239
28	0.004913242144138872
29	0.0030436013282276195
30	0.00469584204926547
31	0.009043843946733498
32	0.004000161745670586
33	0.005478482390809715
34	0.0034784015179744224
35	0.0034784015179744224
36	0.008565563738012014
37	0.00469584204926547
38	0.009130803984682858
39	0.004913242144138872
40	0.0026957611764301774
41	0.0031305613661769803
42	0.0022174809677086944
43	0.0019131208348859324
44	0.001826160796936572
45	0.0015218006641138098
46	0.0011304804933416873
47	0.0026957611764301774
48	0.0025218411005314563
49	3.478401517974422E-4
50	0.001869640815911252
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2299907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.66985945475261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13215233735285	34.385295640904154
2	14.992270172700165	14.29358824080593
3	5.533664711513265	7.913670572026844
4	2.534901879829771	4.833536653722935
5	1.3586957432126194	3.2384417560358107
6	0.8076783515100254	2.3101148100677515
7	0.49251983499733665	1.643484591910069
8	0.35918442759518726	1.3697816945438674
9	0.27109099199115305	1.1630582538900958
>10	1.3273457956276107	11.314365263039527
>50	0.10000215567083842	3.300710691731822
>100	0.07639342639806346	7.877783120561957
>500	0.00970531292019212	3.259462903341991
>1k	0.004394858680841664	3.0967058074172438
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2414	0.1049607658048782	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2377	0.10335200510281503	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2339	0.10169976438177718	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.3480018974680284E-5	0.0
10	0.0	0.0	0.0	8.696003794936057E-5	0.0
11	0.0	0.0	0.0	8.696003794936057E-5	0.0
12	0.0	0.0	0.0	8.696003794936057E-5	0.0
13	0.0	0.0	0.0	8.696003794936057E-5	0.0
14	0.0	0.0	0.0	8.696003794936057E-5	0.0
15	0.0	0.0	0.0	8.696003794936057E-5	0.0
16	0.0	0.0	0.0	8.696003794936057E-5	0.0
17	0.0	0.0	0.0	1.3044005692404084E-4	0.0
18	0.0	0.0	0.0	1.7392007589872113E-4	0.0
19	0.0	0.0	0.0	2.174000948734014E-4	0.0
20	0.0	0.0	0.0	2.174000948734014E-4	0.0
21	0.0	0.0	0.0	3.0436013282276195E-4	0.0
22	0.0	0.0	0.0	4.348001897468028E-4	0.0
23	0.0	0.0	0.0	0.0011304804933416873	0.0
24	0.0	0.0	0.0	0.0022609609866833746	0.0
25	0.0	0.0	0.0	0.0026522811574554972	0.0
26	0.0	0.0	0.0	0.0033479614610503815	0.0
27	0.0	0.0	0.0	0.003869721688746545	0.0
28	0.0	0.0	0.0	0.006000242618505879	0.0
29	0.0	0.0	0.0	0.010043884383151144	0.0
30	0.0	0.0	0.0	0.019739928614504847	0.0
31	0.0	0.0	0.0	0.04252345855723731	0.0
32	0.0	0.0	0.0	0.0661331088604887	0.0
33	0.0	0.0	0.0	0.08865575868937309	0.0
34	0.0	0.0	0.0	0.11461333001725722	0.0
35	0.0	0.0	0.0	0.14439714301491322	0.0
36	0.0	0.0	0.0	0.1865292814013784	0.0
37	0.0	0.0	0.0	0.2456186271879689	0.0
38	0.0	0.0	0.0	0.3350135461999116	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	755	0.0	20.396334	4
TGTAGGA	1405	0.0	19.885561	2
GTATCAA	5205	0.0	19.443563	1
ATACCGT	630	0.0	18.856203	6
GTAGGAC	1475	0.0	18.643543	3
GGACGTG	830	0.0	18.553291	6
AATCACG	490	0.0	18.409245	34
AGGACGT	900	0.0	18.332418	5
CGTCGTA	615	0.0	18.242992	10
TACCGTC	680	0.0	18.116743	7
ACCGTCG	640	0.0	17.87411	8
AGTATCG	75	0.0012917831	17.599121	8
GTCCTAA	695	0.0	17.094158	1
CCGTCGT	670	0.0	17.073774	9
CGCAATA	735	0.0	17.062227	36
CTGTAGG	1575	0.0	17.0419	1
TTAGGAC	765	0.0	16.966843	3
TCACGGA	590	0.0	16.780647	36
AACGCAG	6070	0.0	16.780052	6
ATTTCGT	450	0.0	16.622116	42
>>END_MODULE