FastQCFastQC Report
Thu 2 Feb 2017
SRR4064007_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064007_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514091
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32040.21161211578432207No Hit
TATCAACGCAGAGTACTTTTTTTTT21010.13876312586231607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAT1550.013.486711510
AACCGCG604.0880695E-412.6680777
CGCATCG1700.012.29711313
TTACCCT853.9586375E-612.2897994
TGGCGAG7700.012.21645418
CCGACCA1501.4551915E-1112.0358689
GTCTTAC951.0466265E-611.9934061
CGAGCCG2700.011.96548215
GATATAC2700.011.9563881
TCCAACG1209.984433E-911.87710918
TATACTA1052.7295755E-711.758555
TTAGAAC658.058054E-411.6862693
GTATTAG2600.011.6858831
GTCCTAT2700.011.6047311
CGCCAGT1751.8189894E-1211.40202418
TAGAACC1354.7512003E-911.2553034
TATGGCG8300.011.21885916
GGCGAGG3900.011.20711719
ATGGCGA8500.011.17882417
GTATCAA22200.011.1627821
Produced by FastQC (version 0.11.5)