##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064007_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1514091
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.314316642790956	32.0	32.0	32.0	32.0	32.0
2	31.41478088173036	32.0	32.0	32.0	32.0	32.0
3	31.48786367530089	32.0	32.0	32.0	32.0	32.0
4	31.564643736737093	32.0	32.0	32.0	32.0	32.0
5	31.53724710073569	32.0	32.0	32.0	32.0	32.0
6	35.11234595542804	36.0	36.0	36.0	36.0	36.0
7	35.12884298235707	36.0	36.0	36.0	36.0	36.0
8	35.074809902443114	36.0	36.0	36.0	36.0	36.0
9	35.19526831610518	36.0	36.0	36.0	36.0	36.0
10	35.04584136620586	36.0	36.0	36.0	36.0	36.0
11	35.18539440495981	36.0	36.0	36.0	36.0	36.0
12	35.097914854523275	36.0	36.0	36.0	36.0	36.0
13	35.13443973975144	36.0	36.0	36.0	36.0	36.0
14	35.08368849692654	36.0	36.0	36.0	36.0	36.0
15	35.05027174720674	36.0	36.0	36.0	36.0	36.0
16	35.06676613228663	36.0	36.0	36.0	36.0	36.0
17	35.03788015383488	36.0	36.0	36.0	36.0	36.0
18	35.02910591239232	36.0	36.0	36.0	36.0	36.0
19	35.02869774670083	36.0	36.0	36.0	36.0	36.0
20	35.019748482753016	36.0	36.0	36.0	36.0	36.0
21	35.018479074243224	36.0	36.0	36.0	36.0	36.0
22	34.98991540138605	36.0	36.0	36.0	36.0	36.0
23	34.941092047968056	36.0	36.0	36.0	36.0	36.0
24	34.91734512654788	36.0	36.0	36.0	36.0	36.0
25	34.890680943219394	36.0	36.0	36.0	32.0	36.0
26	34.833706164292636	36.0	36.0	36.0	32.0	36.0
27	34.826139908367466	36.0	36.0	36.0	32.0	36.0
28	34.80245374947741	36.0	36.0	36.0	32.0	36.0
29	34.7600177268077	36.0	36.0	36.0	32.0	36.0
30	34.74688641567779	36.0	36.0	36.0	32.0	36.0
31	34.74091055293242	36.0	36.0	36.0	32.0	36.0
32	34.699238685125266	36.0	36.0	36.0	32.0	36.0
33	34.675452796430335	36.0	36.0	36.0	32.0	36.0
34	34.66531668175823	36.0	36.0	36.0	32.0	36.0
35	34.620715003259384	36.0	36.0	36.0	32.0	36.0
36	34.599719567714224	36.0	36.0	36.0	32.0	36.0
37	34.57803130723318	36.0	36.0	36.0	32.0	36.0
38	34.547606451659775	36.0	36.0	36.0	32.0	36.0
39	34.52927532096816	36.0	36.0	36.0	32.0	36.0
40	34.52278363717901	36.0	36.0	36.0	32.0	36.0
41	34.51103269222259	36.0	36.0	36.0	32.0	36.0
42	34.46069555924974	36.0	36.0	36.0	32.0	36.0
43	34.44485172951956	36.0	36.0	36.0	32.0	36.0
44	34.38826464195349	36.0	36.0	36.0	32.0	36.0
45	34.35993543320712	36.0	36.0	36.0	32.0	36.0
46	34.31927010992074	36.0	36.0	36.0	32.0	36.0
47	34.30210799747175	36.0	36.0	36.0	32.0	36.0
48	34.25100803056091	36.0	36.0	36.0	32.0	36.0
49	34.23688008184449	36.0	36.0	36.0	32.0	36.0
50	33.65509668837606	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	3.0
19	4.0
20	15.0
21	36.0
22	145.0
23	485.0
24	1219.0
25	2904.0
26	5802.0
27	10261.0
28	17135.0
29	26188.0
30	37836.0
31	53580.0
32	76849.0
33	122276.0
34	255645.0
35	903705.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26719521676036	18.156243952090982	12.026132911655777	26.550427919492876
2	15.511028450204876	20.415644046169167	38.03738762182283	26.03593988180312
3	18.230704657538592	24.52923650817029	28.879724559353747	28.360334274937372
4	12.138768409560589	16.416912853983018	36.279721628356555	35.16459710809984
5	13.991827439698143	37.24994072351001	33.690577382733274	15.067654454058573
6	33.68808987576696	36.080682125897404	17.0250777694853	13.206150228850333
7	29.810315106763795	30.55128823253572	20.999280095635	18.639116565065486
8	27.551382314537236	34.01440204056427	19.20584694050754	19.228368704390952
9	27.49023166604583	13.93041036249171	18.455678578882434	40.12367939258003
10	15.76832821138743	27.040505972961675	31.950969338994884	25.240196476656013
11	36.894744107190384	20.840359000879076	23.178461532364963	19.086435359565574
12	24.97473730095305	23.32278794523443	29.421764888480872	22.28070986533165
13	29.897720202736703	19.852689616546527	25.16680074130727	25.082789439409492
14	23.31700667332414	19.954322337935444	25.307974173231674	31.42069681550874
15	24.88294446921286	28.075295982563038	22.064893248129984	24.97686630009412
16	25.149563363481576	25.75620478259319	24.630764414420963	24.463467439504278
17	23.659278075095884	26.26533015518882	25.54760579119749	24.527785978517805
18	24.330636218394318	25.047768060884216	26.98827742395252	23.633318296768945
19	25.798251227964503	24.58676526047642	25.396954344223694	24.218029167335384
20	25.961539731665788	24.582816518524094	25.190907757968194	24.26473599184192
21	26.442069862379476	24.129064897684486	24.51279348467166	24.91607175526438
22	25.84684641843803	24.811239505233512	24.843998499426718	24.49791557690174
23	24.31945292046106	24.709129528504516	25.619387666140938	25.352029884893497
24	25.01936815881296	24.86798940348713	25.53955100215774	24.573091435542175
25	24.89987133863302	24.69855724902447	25.68379421578651	24.717777196555996
26	24.186557710115867	25.530614967787272	26.02493530374545	24.257892018351413
27	24.778449513173477	25.259628702718445	25.18987918670479	24.772042597403285
28	24.263585962196156	25.11634542603628	25.498769505939794	25.12129910582777
29	24.569017615771305	24.8673582815789	25.256317365249686	25.307306737400108
30	24.358844801109626	25.025808688760094	25.691517643367845	24.923828866762438
31	25.28556916341809	24.61997867891154	24.95492056111187	25.139531596558506
32	24.622431523813926	25.333513866964324	24.9545913885458	25.089463220675945
33	24.305773426163274	25.059724984362852	25.583032754456177	25.051468835017698
34	24.885505762689476	24.97539711370166	25.78996730623163	24.349129817377232
35	25.141376153696783	25.036425577558408	25.575709060358797	24.246489208386006
36	24.351853503138436	25.33344429979174	25.404383571415835	24.910318625653993
37	25.04942104884246	25.068641262959606	24.895395140522222	24.986542547675707
38	24.662841864280725	25.258953564714943	25.26879518724843	24.809409383755906
39	25.271709516025165	24.73757038358019	24.977526791169236	25.013193309225407
40	25.75700934579439	24.868201182259504	25.31950728179386	24.05528219015224
41	24.75946852236033	24.866797046054835	25.89972675809075	24.474007673494082
42	25.4344418913537	25.602399890625854	25.157704173323676	23.805454044696774
43	24.51579155243255	24.947211784931863	25.723860669493924	24.81313599314166
44	24.324679553438802	25.340161777050962	25.174448287682317	25.160710381827922
45	24.4124921981553	25.449501837767862	25.22969621515589	24.90830974892095
46	24.5304228654459	24.789789145187655	25.249805987153874	25.42998200221257
47	25.077341716972906	24.895314584626764	25.422440326010857	24.60490337238947
48	25.054357158897268	25.42310080181763	24.71843916357344	24.80410287571166
49	24.800655181182034	25.268858749673896	24.906659797831693	25.023826271312377
50	24.559579674584644	25.97497465432462	24.622522811107846	24.842922859982895
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	25.5
2	31.0
3	70.0
4	109.0
5	148.0
6	187.0
7	189.0
8	191.0
9	239.5
10	288.0
11	445.5
12	603.0
13	1084.0
14	1565.0
15	2274.5
16	2984.0
17	3203.5
18	3423.0
19	3569.5
20	3716.0
21	4181.0
22	4646.0
23	5316.5
24	5987.0
25	7337.0
26	8687.0
27	11494.5
28	14302.0
29	17730.0
30	21158.0
31	24815.0
32	28472.0
33	34174.5
34	39877.0
35	46781.5
36	53686.0
37	59815.5
38	65945.0
39	67928.0
40	69911.0
41	74103.5
42	78296.0
43	80026.5
44	81757.0
45	89114.5
46	96472.0
47	101824.0
48	107176.0
49	110054.0
50	112932.0
51	111041.5
52	109151.0
53	108344.0
54	107537.0
55	106981.0
56	106425.0
57	102849.5
58	99274.0
59	90239.0
60	81204.0
61	71468.0
62	61732.0
63	53548.5
64	45365.0
65	38490.5
66	31616.0
67	27244.0
68	22872.0
69	20206.5
70	17541.0
71	13750.0
72	9959.0
73	8602.0
74	7245.0
75	5657.0
76	4069.0
77	3508.0
78	2947.0
79	2499.5
80	2052.0
81	1651.0
82	1250.0
83	980.5
84	711.0
85	532.0
86	353.0
87	258.0
88	163.0
89	117.5
90	72.0
91	58.0
92	44.0
93	38.0
94	32.0
95	29.5
96	27.0
97	22.0
98	17.0
99	14.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00911437951880039
2	4.623235987797299E-4
3	5.283698271768341E-4
4	0.0
5	0.0
6	6.604622839710426E-5
7	6.604622839710426E-5
8	0.0
9	9.90693425956564E-4
10	0.0011888321111478768
11	0.0
12	6.604622839710426E-5
13	5.944160555739384E-4
14	1.9813868519131283E-4
15	0.004359051074208882
16	0.0011227858827507727
17	0.0
18	2.6418491358841704E-4
19	0.0
20	5.944160555739384E-4
21	0.0
22	1.9813868519131283E-4
23	9.90693425956564E-4
24	5.283698271768341E-4
25	0.0025758029074870663
26	0.006142299240930698
27	0.006736715296504636
28	0.004293004845811778
29	0.0033023114198552136
30	0.004359051074208882
31	0.006406484154519115
32	0.0040288199322233605
33	0.004491143531003091
34	0.002707895364281275
35	0.0029720802778696923
36	0.007925547407652512
37	0.003962773703826256
38	0.007463223808872783
39	0.004359051074208882
40	0.002707895364281275
41	0.0031702189630610053
42	0.001453017024736294
43	0.001453017024736294
44	0.0021134793087073363
45	0.0017172019383247111
46	0.0010567396543536682
47	0.0020474330803102324
48	0.0020474330803102324
49	3.9627737038262567E-4
50	0.0017172019383247111
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1514091.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.922828774846906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5553647771225	46.87627136882876
2	12.389865188475456	14.600918100879511
3	3.512520897923922	6.209030023093284
4	1.4665739591695859	3.456587451271953
5	0.7634086598122742	2.249109887367699
6	0.47720607208940385	1.6870999005624714
7	0.3275226632751886	1.3508993265632045
8	0.2400650967050006	1.131625167037265
9	0.17511016217358147	0.9286187492240652
>10	0.9672265753582994	10.231804228316626
>50	0.06401895239763525	2.60592432662048
>100	0.05615557708869885	6.554368085227702
>500	0.004510380371410784	1.8197550847699473
>1k	4.510380371410784E-4	0.29798830023707373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.981386851913128E-4
2	0.0	0.0	0.0	0.0	2.641849135884171E-4
3	0.0	0.0	0.0	0.0	2.641849135884171E-4
4	0.0	0.0	0.0	0.0	2.641849135884171E-4
5	0.0	0.0	0.0	0.0	2.641849135884171E-4
6	0.0	0.0	0.0	0.0	3.962773703826256E-4
7	0.0	0.0	0.0	0.0	3.962773703826256E-4
8	0.0	0.0	0.0	0.0	3.962773703826256E-4
9	0.0	0.0	0.0	0.0	3.962773703826256E-4
10	0.0	0.0	0.0	0.0	3.962773703826256E-4
11	0.0	0.0	0.0	0.0	3.962773703826256E-4
12	0.0	0.0	0.0	0.0	7.26508512368147E-4
13	0.0	0.0	0.0	0.0	8.586009691623555E-4
14	0.0	0.0	0.0	0.0	8.586009691623555E-4
15	0.0	0.0	0.0	0.0	0.0011227858827507725
16	0.0	0.0	0.0	0.0	0.0011227858827507725
17	0.0	0.0	0.0	0.0	0.0011227858827507725
18	0.0	0.0	0.0	6.604622839710427E-5	0.0013209245679420853
19	0.0	0.0	0.0	6.604622839710427E-5	0.0013209245679420853
20	0.0	0.0	0.0	1.3209245679420855E-4	0.0016511557099276068
21	0.0	0.0	0.0	2.641849135884171E-4	0.0016511557099276068
22	0.0	0.0	0.0	3.962773703826256E-4	0.0016511557099276068
23	0.0	0.0	0.0	7.26508512368147E-4	0.0016511557099276068
24	0.0	0.0	0.0	0.0011227858827507725	0.0016511557099276068
25	0.0	0.0	0.0	0.0013869707963391896	0.0016511557099276068
26	0.0	0.0	0.0	0.0017832481667218153	0.0016511557099276068
27	0.0	0.0	0.0	0.001981386851913128	0.0016511557099276068
28	0.0	0.0	0.0	0.003038126506266796	0.001717201938324711
29	0.0	0.0	0.0	0.006406484154519114	0.001717201938324711
30	0.0	0.0	0.0	0.012020413568272976	0.001717201938324711
31	0.0	0.0	0.0	0.028862201809534565	0.001717201938324711
32	0.0	0.0	0.0	0.04755328444591508	0.001717201938324711
33	0.0	0.0	0.0	0.06525367365633902	0.001717201938324711
34	0.0	0.0	0.0	0.08724706771257473	0.001717201938324711
35	0.0	0.0	0.0	0.11016510896636993	0.001717201938324711
36	0.0	0.0	0.0	0.14450914773286413	0.001717201938324711
37	0.0	0.0	0.0	0.19536474359863443	0.001717201938324711
38	0.0	0.0	0.0	0.2649114221007852	0.0017832481667218153
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2355	0.0	21.205818	1
ATACCGT	275	0.0	20.798502	6
CGGATCG	55	0.004480853	20.002523	26
ACCGTCG	265	0.0	19.923092	8
CCGTCGT	300	0.0	19.799227	9
GGTATCA	1185	0.0	19.493553	1
TAATACC	260	0.0	19.460136	4
CGTCGTA	295	0.0	19.388432	10
TAGGACG	965	0.0	19.14888	4
TACCGTC	280	0.0	18.855785	7
GTAGGAC	1160	0.0	18.774508	3
GTCGTAG	305	0.0	18.752747	11
GATATAC	435	0.0	18.712532	1
TGTAGGA	1130	0.0	18.688301	2
ACGGTAT	310	0.0	18.450891	9
TTAGGAC	685	0.0	18.30525	3
TACGACG	320	0.0	17.873713	5
CATATAG	125	4.8598304E-7	17.59815	2
GTATAAA	240	0.0	17.416561	1
TAACGGC	140	8.367351E-8	17.287325	36
>>END_MODULE