FastQCFastQC Report
Thu 2 Feb 2017
SRR4064006_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064006_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288718
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29620.22984081854990776No Hit
TATCAACGCAGAGTACTTTTTTTTT21210.16458216615271923No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14440.11204933895545806No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14370.1115061634896075No Hit
GATTAAGAGGGACGGCCGGGGGCAT13320.10335853150184912No Hit
GAATAGGACCGCGGTTCTATTTTGT13270.10297054902624159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT1600.014.2443242
TTTTAGT551.9624953E-413.8158954
ACCGTCC707.2551793E-613.5723438
GCGTTAT1700.013.4105861
CCGACCA1500.013.3008989
GCCATAA655.455568E-513.1526911
GTTATTC1950.013.1486063
CGGTTCT2950.012.56016812
CCGTCGT2800.012.5544189
TCCCCGA2200.012.52357213
TTGCGCG1301.9645086E-1012.4248818
CTCGTAG1155.315087E-912.3921410
TCAGTTA853.9388597E-612.2949478
CGTCGTA2650.012.18950210
ACCGTCG2650.012.1895028
GCTCGTA1251.4078978E-912.160829
CGTAGAA550.003063731612.09313315
GGTTCTA3150.012.06430513
GTCGTAG2600.012.05803811
CGCATCG2050.012.04959313
Produced by FastQC (version 0.11.5)