##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064006_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1288718
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27950024753282	32.0	32.0	32.0	32.0	32.0
2	30.90384940692999	32.0	32.0	32.0	32.0	32.0
3	30.934008836688864	32.0	32.0	32.0	32.0	32.0
4	30.962028931077242	32.0	32.0	32.0	32.0	32.0
5	30.876223502736828	32.0	32.0	32.0	32.0	32.0
6	34.53043567328151	36.0	36.0	36.0	32.0	36.0
7	34.466438739895	36.0	36.0	36.0	32.0	36.0
8	34.43834570480121	36.0	36.0	36.0	32.0	36.0
9	34.56560861259019	36.0	36.0	36.0	32.0	36.0
10	34.283756415290235	36.0	36.0	36.0	32.0	36.0
11	34.54319952076405	36.0	36.0	36.0	32.0	36.0
12	34.36140490006348	36.0	36.0	36.0	32.0	36.0
13	34.45360893539161	36.0	36.0	36.0	32.0	36.0
14	34.35769190777191	36.0	36.0	36.0	32.0	36.0
15	34.29632937539477	36.0	36.0	36.0	32.0	36.0
16	34.28716057353122	36.0	36.0	36.0	32.0	36.0
17	34.22558465079249	36.0	36.0	36.0	32.0	36.0
18	34.213779120024704	36.0	36.0	36.0	32.0	36.0
19	34.2376920319263	36.0	36.0	36.0	32.0	36.0
20	34.21634833997818	36.0	36.0	36.0	32.0	36.0
21	34.18927802669009	36.0	36.0	36.0	32.0	36.0
22	34.177640880316716	36.0	36.0	36.0	32.0	36.0
23	34.13694229459044	36.0	36.0	36.0	32.0	36.0
24	34.107793947162996	36.0	36.0	36.0	32.0	36.0
25	33.726933277877706	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	12.0
5	13.0
6	40.0
7	16.0
8	80.0
9	78.0
10	109.0
11	39.0
12	70.0
13	65.0
14	113.0
15	204.0
16	336.0
17	499.0
18	596.0
19	746.0
20	1143.0
21	1664.0
22	2676.0
23	4181.0
24	6043.0
25	9088.0
26	12821.0
27	17296.0
28	24282.0
29	32942.0
30	44099.0
31	60630.0
32	86266.0
33	123666.0
34	267826.0
35	591072.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84801486666806	17.82494249955948	11.630497472930353	25.696545160842106
2	16.363805673357103	20.093818749005745	37.82470870791639	25.717666869720755
3	18.018460535658164	24.64927698175286	29.20015985163285	28.132102630956123
4	12.04718024327313	16.05587134071818	37.34355054610356	34.55339786990513
5	13.953506419330006	37.31297648315081	34.31066701432864	14.422850083190545
6	32.71437174635794	36.954932498083124	17.483446586721882	12.847249168837054
7	29.674496073913044	31.109029340291656	21.320549846208532	17.895924739586768
8	27.88290991117566	32.781172934618674	19.852003418530078	19.48391373567559
9	27.472539416001162	14.208702767368543	18.888161730399133	39.43059608623116
10	15.444004052810763	26.562603115695328	32.49515720169721	25.4982356297967
11	36.15131037524029	21.575928473604613	23.40787622629322	18.864884924861883
12	24.231843412755907	24.379445633699582	29.25816102074584	22.130549932798672
13	28.688407485053187	19.891606491187204	25.85216243497166	25.567823588787945
14	23.33722604159711	20.26309945507261	25.83334756863616	30.56632693469413
15	25.056270939039493	27.497563986599015	22.71055502284612	24.735610051515376
16	25.419005474927225	25.95562754344185	24.229755930524096	24.395611051106826
17	23.534463080317835	26.22026494652776	25.579793751038466	24.66547822211594
18	24.249055519532206	25.35587402785489	26.563644199744513	23.831426252868386
19	25.02486824296254	25.378265194477034	25.946604523884776	23.650262038675653
20	25.1723961196895	24.801200870988684	25.418332385931237	24.60807062339058
21	26.503234222971138	24.42742993811103	24.875329052097005	24.194006786820832
22	25.456279270947146	24.670103661183475	25.243117274819006	24.630499793050376
23	24.24033690841642	24.65052275009338	25.735938787412373	25.373201554077824
24	24.279340072590152	25.603636651658906	25.49142787033169	24.625595405419258
25	24.46670549407882	24.961289070083478	25.700795961949137	24.871209473888563
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	168.0
1	168.0
2	346.5
3	525.0
4	525.0
5	525.0
6	1448.0
7	2371.0
8	2371.0
9	2371.0
10	2384.0
11	2397.0
12	2397.0
13	2397.0
14	3171.5
15	3946.0
16	3946.0
17	3946.0
18	7038.0
19	10130.0
20	10130.0
21	10130.0
22	15934.5
23	21739.0
24	21739.0
25	21739.0
26	32558.0
27	43377.0
28	43377.0
29	43377.0
30	53800.5
31	64224.0
32	64224.0
33	64224.0
34	77764.5
35	91305.0
36	91305.0
37	91305.0
38	101677.5
39	112050.0
40	112050.0
41	112050.0
42	124985.0
43	137920.0
44	137920.0
45	137920.0
46	153797.0
47	169674.0
48	169674.0
49	169674.0
50	170542.5
51	171411.0
52	171411.0
53	171411.0
54	156873.5
55	142336.0
56	142336.0
57	142336.0
58	128658.0
59	114980.0
60	114980.0
61	114980.0
62	100576.5
63	86173.0
64	86173.0
65	86173.0
66	70796.0
67	55419.0
68	55419.0
69	55419.0
70	41710.5
71	28002.0
72	28002.0
73	28002.0
74	21555.0
75	15108.0
76	15108.0
77	15108.0
78	11908.5
79	8709.0
80	8709.0
81	8709.0
82	6016.0
83	3323.0
84	3323.0
85	3323.0
86	2544.0
87	1765.0
88	1765.0
89	1765.0
90	1251.5
91	738.0
92	738.0
93	738.0
94	515.0
95	292.0
96	292.0
97	292.0
98	464.0
99	636.0
100	636.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0362375632217444
2	0.004888579192655026
3	0.0017847193877946921
4	0.003336649290224859
5	0.008535614463365919
6	0.012570632209684353
7	0.02211500110962988
8	0.03453044032907122
9	0.046480300577783504
10	0.056102265972850535
11	0.05354158163384076
12	0.061844406611842165
13	0.06347393300939383
14	0.06518105590206702
15	0.05843016082649579
16	0.06572423136791758
17	0.05897333629234635
18	0.07550138975322762
19	0.07224233695812426
20	0.07651014418980723
21	0.07200954747275976
22	0.07488061779225556
23	0.07829486357760193
24	0.07294070541421785
25	0.07511340727762009
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1288718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.36809416874315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97838854490112	37.50908240474034
2	13.97904154081263	13.802372783512205
3	4.29611716216424	6.362733498650333
4	1.819510095211567	3.593029828855338
5	1.007349245676141	2.486545621067605
6	0.5913707889232174	1.7516909277723192
7	0.3737708040161703	1.2916646575137998
8	0.27893123326388014	1.1016242712299915
9	0.20997825084091543	0.9329603454812054
>10	1.2328826097759185	11.36169961568632
>50	0.11408859253076353	3.9420965646706856
>100	0.10550154662866133	10.71175289568197
>500	0.01070761201604498	3.5765428291747723
>1k	0.002361973238833451	1.5762037559630433
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2962	0.22984081854990776	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2121	0.16458216615271923	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1444	0.11204933895545806	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1437	0.1115061634896075	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1332	0.10335853150184912	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1327	0.10297054902624159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.759649512150835E-5
2	0.0	0.0	0.0	0.0	7.759649512150835E-5
3	0.0	0.0	0.0	0.0	7.759649512150835E-5
4	0.0	0.0	0.0	0.0	7.759649512150835E-5
5	0.0	0.0	0.0	0.0	7.759649512150835E-5
6	0.0	0.0	0.0	0.0	1.551929902430167E-4
7	0.0	0.0	0.0	0.0	1.551929902430167E-4
8	0.0	0.0	0.0	0.0	1.551929902430167E-4
9	0.0	0.0	0.0	0.0	1.551929902430167E-4
10	0.0	0.0	0.0	0.0	1.551929902430167E-4
11	0.0	0.0	0.0	0.0	1.551929902430167E-4
12	0.0	0.0	0.0	0.0	3.103859804860334E-4
13	0.0	0.0	0.0	0.0	3.103859804860334E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	160	0.0	14.244324	2
TTTTAGT	55	1.9624953E-4	13.815895	4
ACCGTCC	70	7.2551793E-6	13.572343	8
GCGTTAT	170	0.0	13.410586	1
CCGACCA	150	0.0	13.300898	9
GCCATAA	65	5.455568E-5	13.152691	1
GTTATTC	195	0.0	13.148606	3
CGGTTCT	295	0.0	12.560168	12
CCGTCGT	280	0.0	12.554418	9
TCCCCGA	220	0.0	12.523572	13
TTGCGCG	130	1.9645086E-10	12.42488	18
CTCGTAG	115	5.315087E-9	12.39214	10
TCAGTTA	85	3.9388597E-6	12.294947	8
CGTCGTA	265	0.0	12.189502	10
ACCGTCG	265	0.0	12.189502	8
GCTCGTA	125	1.4078978E-9	12.16082	9
CGTAGAA	55	0.0030637316	12.093133	15
GGTTCTA	315	0.0	12.064305	13
GTCGTAG	260	0.0	12.058038	11
CGCATCG	205	0.0	12.049593	13
>>END_MODULE