FastQCFastQC Report
Thu 2 Feb 2017
SRR4064006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288718
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13510.10483286490915779No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13300.10320333851160611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT300.00574480629.33369410
CGGTCGA500.00258034422.00027310
GTATAGG2200.020.0010221
ATCGGTA550.00448344920.00024629
GTATCAA27100.019.9715021
TCGGTCG708.123045E-418.8573769
GTATATC1102.797422E-617.9995233
AATAGCG2500.017.5995355
TACGCTA3550.017.3523279
ATAGCGT2450.017.0607746
ACCGTCG3150.016.7614618
TAGGACG1053.7755122E-516.7614614
GTATTAC1451.2486817E-716.6905081
TACCGTC3300.016.6662257
ACGCTAT3750.016.4268710
CGTCTTA3500.016.34305815
GATATAT3100.016.3234161
CGTCGTA3300.016.00019810
TAATACT3450.015.94160654
AACGCAG33650.015.8866536
Produced by FastQC (version 0.11.5)