##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1288718
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31730060416631	32.0	32.0	32.0	32.0	32.0
2	31.390603685212746	32.0	32.0	32.0	32.0	32.0
3	31.473769280789124	32.0	32.0	32.0	32.0	32.0
4	31.5675663721621	32.0	32.0	32.0	32.0	32.0
5	31.507825606533004	32.0	32.0	32.0	32.0	32.0
6	35.09424792700963	36.0	36.0	36.0	36.0	36.0
7	35.11117948224515	36.0	36.0	36.0	36.0	36.0
8	35.05760841394316	36.0	36.0	36.0	36.0	36.0
9	35.17905158459803	36.0	36.0	36.0	36.0	36.0
10	35.02611354850324	36.0	36.0	36.0	36.0	36.0
11	35.17434147734415	36.0	36.0	36.0	36.0	36.0
12	35.07223302537871	36.0	36.0	36.0	36.0	36.0
13	35.130969692360935	36.0	36.0	36.0	36.0	36.0
14	35.06990125070031	36.0	36.0	36.0	36.0	36.0
15	35.042106186147784	36.0	36.0	36.0	36.0	36.0
16	35.05622952422485	36.0	36.0	36.0	36.0	36.0
17	35.022360981999164	36.0	36.0	36.0	36.0	36.0
18	35.010993871428816	36.0	36.0	36.0	36.0	36.0
19	35.01563026201232	36.0	36.0	36.0	36.0	36.0
20	35.003286211568394	36.0	36.0	36.0	36.0	36.0
21	35.00482727796151	36.0	36.0	36.0	36.0	36.0
22	34.987025866015685	36.0	36.0	36.0	36.0	36.0
23	34.93351454701494	36.0	36.0	36.0	36.0	36.0
24	34.90686946252012	36.0	36.0	36.0	32.0	36.0
25	34.8820137532028	36.0	36.0	36.0	32.0	36.0
26	34.8237775836141	36.0	36.0	36.0	32.0	36.0
27	34.81363184187697	36.0	36.0	36.0	32.0	36.0
28	34.79348080805886	36.0	36.0	36.0	32.0	36.0
29	34.76461801573347	36.0	36.0	36.0	32.0	36.0
30	34.748498119836924	36.0	36.0	36.0	32.0	36.0
31	34.73856033670671	36.0	36.0	36.0	32.0	36.0
32	34.6987052248824	36.0	36.0	36.0	32.0	36.0
33	34.68413648292334	36.0	36.0	36.0	32.0	36.0
34	34.6697089665854	36.0	36.0	36.0	32.0	36.0
35	34.623255824780905	36.0	36.0	36.0	32.0	36.0
36	34.590680816128895	36.0	36.0	36.0	32.0	36.0
37	34.595838655159625	36.0	36.0	36.0	32.0	36.0
38	34.554401350799786	36.0	36.0	36.0	32.0	36.0
39	34.550122680058784	36.0	36.0	36.0	32.0	36.0
40	34.52159743248717	36.0	36.0	36.0	32.0	36.0
41	34.508099522160784	36.0	36.0	36.0	32.0	36.0
42	34.4376046582728	36.0	36.0	36.0	32.0	36.0
43	34.42728742828144	36.0	36.0	36.0	32.0	36.0
44	34.37230099990844	36.0	36.0	36.0	32.0	36.0
45	34.335964113172935	36.0	36.0	36.0	32.0	36.0
46	34.32328562183503	36.0	36.0	36.0	32.0	36.0
47	34.28042830161447	36.0	36.0	36.0	32.0	36.0
48	34.234913301436	36.0	36.0	36.0	32.0	36.0
49	34.226356735918955	36.0	36.0	36.0	32.0	36.0
50	33.629335510173675	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	1.0
20	5.0
21	47.0
22	112.0
23	408.0
24	1000.0
25	2362.0
26	4734.0
27	8877.0
28	14407.0
29	22112.0
30	31763.0
31	45137.0
32	66188.0
33	107469.0
34	226567.0
35	757527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94086560138196	18.829014587069604	12.139387726436377	26.090732085112062
2	15.76110994715646	20.93217248256008	37.221329856988774	26.08538771329469
3	17.239653453246063	25.246000861323104	29.646818730285595	27.867526955145244
4	11.69076555150157	16.778534947133508	37.42758307092785	34.10311643043707
5	13.645498860107486	37.6954461720873	34.036616234117936	14.622438733687277
6	32.74163235597658	37.32946177351996	17.156793516637517	12.772112353865946
7	29.511001648152114	31.275781475515164	21.388963898950582	17.824252977382137
8	27.802979395026682	33.065573694167384	19.617169931668528	19.514276979137406
9	27.448415109478457	14.080768153357528	18.842772683500673	39.628044053663345
10	15.347141984730456	26.851445904389585	32.35548133830058	25.445930772579377
11	36.41730496299808	21.65797455919337	23.205560258768994	18.71916021903956
12	24.2152466367713	24.45703750319116	29.224492266339315	22.103223593698228
13	28.83577827525869	19.843487504995284	25.763027511901388	25.557706707844634
14	23.239327328371274	20.276912141735316	25.83494217482261	30.6488183550708
15	25.16524891709385	27.553617628206624	22.577526147275236	24.70360730742429
16	25.55307245229726	26.056056056056054	24.075005237795935	24.31586625385075
17	23.51422110966092	26.267344756572037	25.5698298619248	24.64860427184225
18	24.323862939758737	25.346274359479732	26.499591066470714	23.830271634290824
19	25.120002979705415	25.460729189783954	25.85142754272075	23.56784028778988
20	25.26229697736731	24.769556556900653	25.42346577316404	24.544680692568
21	26.53932047197292	24.495739176452876	24.83592221106557	24.129018140508627
22	25.503621824841023	24.581462930128072	25.355489770818217	24.559425474212684
23	24.16357829261025	24.554048097934047	25.83712008343266	25.44525352602304
24	24.40135918759132	25.477162839457414	25.483525786619342	24.637952186331923
25	24.509486684653773	24.80055497745404	25.843000044230585	24.846958293661604
26	24.33733593448088	25.456636418238453	26.228677496504126	23.97735015077654
27	24.9757498156986	25.40084584642843	25.04225352112676	24.58115081674621
28	24.22319376351195	24.899935903106606	26.127476770696077	24.749393562685366
29	24.27375749599593	25.194306147027053	25.941267397539203	24.590668959437817
30	24.365705576886665	25.285874804838453	26.09725020098147	24.251169417293408
31	24.853118862168806	24.964089686154335	25.268056621504904	24.91473483017196
32	24.304912135306818	25.268839732875193	25.171298516473367	25.254949615344625
33	24.227920121800754	24.9662052578566	25.540056275423655	25.265818344918994
34	24.830679980507245	24.95499284541439	25.939718348330544	24.27460882574782
35	25.422388593626206	24.918696064121278	25.609090831492303	24.04982451076021
36	24.355885208232937	25.190493152024363	25.42353152070922	25.03009011903348
37	25.425901582862437	25.122800265077856	25.080043269148693	24.371254882911014
38	24.400926576207787	25.05860943726404	25.734140918738333	24.806323067789837
39	24.909518976183158	25.337557850431608	24.753776767949542	24.99914640543569
40	25.021572327824888	25.449216567547772	25.619932830910198	23.909278273717145
41	24.153004022745897	25.71430123413871	25.784994164535274	24.34770057858012
42	25.328083582163675	25.755802764965512	25.112672034366522	23.803441618504287
43	24.556879713075638	24.90994814937821	25.583416491943055	24.949755645603094
44	24.37425312330255	25.33429037014045	25.201132924652754	25.090323581904244
45	24.62726060796057	25.536082042240952	25.344804306969337	24.491853042829142
46	24.55158264264968	25.251901336153747	25.196962207621798	24.999553813574778
47	24.687802875635626	25.234327289206316	25.565826976097412	24.512042859060642
48	25.221230697602238	25.736556219445955	24.703965236284628	24.338247846667187
49	24.372650275662192	25.719883760179712	25.199753242571088	24.707712721587008
50	24.393014693044567	25.86115519067591	25.06523991138391	24.680590204895612
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	40.0
2	21.0
3	83.5
4	146.0
5	181.5
6	217.0
7	198.0
8	179.0
9	241.0
10	303.0
11	453.5
12	604.0
13	1154.5
14	1705.0
15	2284.5
16	2864.0
17	3247.5
18	3631.0
19	3724.0
20	3817.0
21	4372.0
22	4927.0
23	5643.0
24	6359.0
25	7977.5
26	9596.0
27	12424.0
28	15252.0
29	17575.0
30	19898.0
31	21580.0
32	23262.0
33	27115.5
34	30969.0
35	35619.5
36	40270.0
37	47494.5
38	54719.0
39	59054.5
40	63390.0
41	68868.0
42	74346.0
43	74425.5
44	74505.0
45	80410.0
46	86315.0
47	89888.5
48	93462.0
49	95770.5
50	98079.0
51	94385.0
52	90691.0
53	89022.0
54	87353.0
55	86605.0
56	85857.0
57	83424.0
58	80991.0
59	73723.5
60	66456.0
61	58047.0
62	49638.0
63	43108.5
64	36579.0
65	31288.0
66	25997.0
67	22352.5
68	18708.0
69	16975.5
70	15243.0
71	11648.5
72	8054.0
73	6760.5
74	5467.0
75	4288.0
76	3109.0
77	2682.0
78	2255.0
79	1875.5
80	1496.0
81	1167.0
82	838.0
83	703.5
84	569.0
85	399.0
86	229.0
87	165.0
88	101.0
89	84.5
90	68.0
91	57.0
92	46.0
93	38.0
94	30.0
95	25.5
96	21.0
97	18.0
98	15.0
99	13.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008613210958487426
2	6.207719609720669E-4
3	2.3278948536452506E-4
4	0.0
5	0.0
6	3.1038598048603345E-4
7	1.5519299024301672E-4
8	0.0
9	0.0013191404170656421
10	6.983684560935751E-4
11	7.759649512150836E-5
12	7.759649512150836E-5
13	2.3278948536452506E-4
14	4.655789707290501E-4
15	0.004345403726804468
16	6.207719609720669E-4
17	0.0
18	0.0
19	0.0
20	5.431754658505584E-4
21	0.0
22	2.3278948536452506E-4
23	0.001086350931701117
24	5.431754658505584E-4
25	0.0013191404170656421
26	0.004345403726804468
27	0.0052765616682625685
28	0.003414245785346367
29	0.0023278948536452505
30	0.003259052795103351
31	0.0070612810560572595
32	0.0024830878438882676
33	0.004345403726804468
34	0.0026382808341312842
35	0.0027158773292527924
36	0.006906088065814243
37	0.003103859804860334
38	0.00799243899751536
39	0.0038798247560754176
40	0.0023278948536452505
41	0.0035694387755893842
42	0.0017071228926731836
43	0.0012415439219441338
44	0.0013967369121871502
45	0.0012415439219441338
46	0.0014743334073086586
47	0.0022502983585237423
48	0.0013967369121871502
49	2.3278948536452506E-4
50	0.0010087544365796086
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1288718.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.60314771838931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.05278642289497	40.532191063170465
2	13.496445158207962	14.199109966607073
3	4.140049931867163	6.533389743825479
4	1.772441289428013	3.7294396387981683
5	0.8983738720171365	2.3628646748029403
6	0.5502083913754968	1.736561597245357
7	0.3607379699180105	1.328316690346029
8	0.2509090505821415	1.0558884681322565
9	0.1892285863800982	0.8958617353704867
>10	1.0910264228512248	10.705986631756813
>50	0.10035801367261196	3.6638636354922878
>100	0.08763200116414788	9.45093430771701
>500	0.008169074700969683	2.8320047562746753
>1k	0.001633814940193937	0.9735870904609687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1351	0.10483286490915779	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1330	0.10320333851160611	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.759649512150835E-5
7	0.0	0.0	0.0	0.0	7.759649512150835E-5
8	0.0	0.0	0.0	0.0	7.759649512150835E-5
9	0.0	0.0	0.0	0.0	7.759649512150835E-5
10	0.0	0.0	0.0	0.0	7.759649512150835E-5
11	0.0	0.0	0.0	0.0	7.759649512150835E-5
12	0.0	0.0	0.0	0.0	1.551929902430167E-4
13	0.0	0.0	0.0	0.0	3.103859804860334E-4
14	0.0	0.0	0.0	0.0	3.103859804860334E-4
15	0.0	0.0	0.0	0.0	3.879824756075418E-4
16	0.0	0.0	0.0	0.0	3.879824756075418E-4
17	0.0	0.0	0.0	0.0	4.655789707290501E-4
18	0.0	0.0	0.0	1.551929902430167E-4	4.655789707290501E-4
19	0.0	0.0	0.0	2.3278948536452506E-4	5.431754658505584E-4
20	0.0	0.0	0.0	2.3278948536452506E-4	5.431754658505584E-4
21	0.0	0.0	0.0	2.3278948536452506E-4	6.207719609720668E-4
22	0.0	0.0	0.0	3.879824756075418E-4	6.207719609720668E-4
23	0.0	0.0	0.0	7.759649512150836E-4	6.207719609720668E-4
24	0.0	0.0	0.0	0.0015519299024301671	6.983684560935751E-4
25	0.0	0.0	0.0	0.0019399123780377088	6.983684560935751E-4
26	0.0	0.0	0.0	0.0020951053682807257	8.535614463365919E-4
27	0.0	0.0	0.0	0.002638280834131284	8.535614463365919E-4
28	0.0	0.0	0.0	0.005043772182898043	8.535614463365919E-4
29	0.0	0.0	0.0	0.007604456521907818	8.535614463365919E-4
30	0.0	0.0	0.0	0.013346597160899436	8.535614463365919E-4
31	0.0	0.0	0.0	0.030883405058360323	8.535614463365919E-4
32	0.0	0.0	0.0	0.048342616460699706	9.311579414581002E-4
33	0.0	0.0	0.0	0.06626740683376814	9.311579414581002E-4
34	0.0	0.0	0.0	0.08520095164341616	9.311579414581002E-4
35	0.0	0.0	0.0	0.1077039352286536	9.311579414581002E-4
36	0.0	0.0	0.0	0.14107042813090218	9.311579414581002E-4
37	0.0	0.0	0.0	0.19670711513302366	9.311579414581002E-4
38	0.0	0.0	0.0	0.26980301353748454	9.311579414581002E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	30	0.005744806	29.333694	10
CGGTCGA	50	0.002580344	22.000273	10
GTATAGG	220	0.0	20.001022	1
ATCGGTA	55	0.004483449	20.000246	29
GTATCAA	2710	0.0	19.971502	1
TCGGTCG	70	8.123045E-4	18.857376	9
GTATATC	110	2.797422E-6	17.999523	3
AATAGCG	250	0.0	17.599535	5
TACGCTA	355	0.0	17.352327	9
ATAGCGT	245	0.0	17.060774	6
ACCGTCG	315	0.0	16.761461	8
TAGGACG	105	3.7755122E-5	16.761461	4
GTATTAC	145	1.2486817E-7	16.690508	1
TACCGTC	330	0.0	16.666225	7
ACGCTAT	375	0.0	16.42687	10
CGTCTTA	350	0.0	16.343058	15
GATATAT	310	0.0	16.323416	1
CGTCGTA	330	0.0	16.000198	10
TAATACT	345	0.0	15.9416065	4
AACGCAG	3365	0.0	15.886653	6
>>END_MODULE