FastQCFastQC Report
Thu 2 Feb 2017
SRR4064005_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064005_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211483
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58140.4799076833929985No Hit
TATCAACGCAGAGTACTTTTTTTTT40590.3350439089941831No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31580.2606722504566717No Hit
GTACATGGGGTGGTATCAACGCAAA25130.2074317179853122No Hit
GGTATCAACGCAGAGTACTTTTTTT21050.1737539858173825No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20100.1659123570037714No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19650.16219790124995562No Hit
GTATCAACGCAGAGTACATGGGGTG15100.12464062640581833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAG1500.015.83557117
AACCGTG508.7101085E-515.2002657
CGCCAGT1650.014.39597318
GTATACG400.00528628614.24671651
CGTTATT1006.6211214E-1014.2449512
ATAATAC759.697287E-713.9295483
AACCGCG707.258068E-613.5716657
GCATCGC1750.013.03041314
CGAGCCG2300.012.80615815
TCGTTCA604.08599E-412.66845716
CGTTTCC751.4757976E-512.66845714
ATCGCCA1800.012.66845616
CGCATCG1800.012.66793313
GCTAGAC604.1002542E-412.6632253
CCTATAC1001.4464968E-712.3466453
TTAGGAC3400.012.2907783
CAACGGA550.003064525812.09261914
GTACTAA550.003073071112.0881221
AATCGAA802.8646231E-511.8752077
GTAATAG802.8731125E-511.8717743
Produced by FastQC (version 0.11.5)