##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064005_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1211483
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215089274880455	32.0	32.0	32.0	32.0	32.0
2	30.840199160863172	32.0	32.0	32.0	32.0	32.0
3	30.868901998624825	32.0	32.0	32.0	32.0	32.0
4	30.881701187717862	32.0	32.0	32.0	32.0	32.0
5	30.813732425465318	32.0	32.0	32.0	32.0	32.0
6	34.480019117065616	36.0	36.0	36.0	32.0	36.0
7	34.37695287511257	36.0	36.0	36.0	32.0	36.0
8	34.324429645319	36.0	36.0	36.0	32.0	36.0
9	34.45984384428011	36.0	36.0	36.0	32.0	36.0
10	34.16511416173401	36.0	36.0	36.0	32.0	36.0
11	34.48057628542868	36.0	36.0	36.0	32.0	36.0
12	34.279957704730485	36.0	36.0	36.0	32.0	36.0
13	34.353954615954166	36.0	36.0	36.0	32.0	36.0
14	34.26303216801226	36.0	36.0	36.0	32.0	36.0
15	34.2196819930614	36.0	36.0	36.0	32.0	36.0
16	34.203503474667	36.0	36.0	36.0	32.0	36.0
17	34.12855731363956	36.0	36.0	36.0	32.0	36.0
18	34.12918877111771	36.0	36.0	36.0	32.0	36.0
19	34.12953462822012	36.0	36.0	36.0	32.0	36.0
20	34.105122399571435	36.0	36.0	36.0	32.0	36.0
21	34.089081728757236	36.0	36.0	36.0	32.0	36.0
22	34.05991912391672	36.0	36.0	36.0	32.0	36.0
23	34.024374258656536	36.0	36.0	36.0	32.0	36.0
24	34.00775743448319	36.0	36.0	36.0	32.0	36.0
25	33.62167029995469	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	12.0
5	24.0
6	40.0
7	21.0
8	68.0
9	81.0
10	107.0
11	28.0
12	77.0
13	66.0
14	170.0
15	259.0
16	443.0
17	537.0
18	694.0
19	954.0
20	1425.0
21	2048.0
22	3211.0
23	4789.0
24	6912.0
25	9867.0
26	13854.0
27	18144.0
28	24184.0
29	32003.0
30	42578.0
31	57140.0
32	80222.0
33	113489.0
34	244741.0
35	553288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.964212577619236	17.465153917294227	11.507547232131062	25.063086272955477
2	16.008601404951172	20.155683778674792	39.18674624204452	24.648968574329512
3	18.81850772338295	25.25954413343998	28.70153229474407	27.220415848432992
4	11.97676463086157	15.951326904011708	36.69014032188432	35.381768143242404
5	13.53447699428665	38.08570935624287	33.84960701617091	14.530206633299569
6	32.865660158042985	36.440119671497776	17.639988244190615	13.054231926268619
7	29.31605880517113	31.24796686883765	21.203597135369794	18.232377190621424
8	27.82226200434172	33.71743813793257	19.50206475255586	18.958235105169848
9	27.82271750898726	14.079831131217684	18.823815056970915	39.273636302824144
10	15.696431698123181	27.548456687138454	32.18739779248281	24.567713822255552
11	36.3119425173439	21.29360753221011	23.422695738354808	18.97175421209118
12	25.077353095197587	24.10443039543001	29.799402320345063	21.018814189027342
13	30.029801089292583	20.264658121705402	25.540227340682286	24.165313448319726
14	23.258922007701596	20.507773458730707	26.38110845507154	29.852196078496156
15	24.351180850027006	28.770221137851458	23.19188792290322	23.68671008921831
16	24.32042435585893	26.416598247929677	25.530320926552143	23.73265646965925
17	22.66556095836496	26.717392686962032	26.31704148140684	24.30000487326617
18	23.316679388430135	25.65459255661185	28.152766900945377	22.875961154012643
19	24.824378799894266	25.292591858313507	26.630402458366376	23.252626883425854
20	25.427447688928282	24.854873899111027	26.302789180329768	23.414889231630923
21	26.32794571741533	24.488126923706005	25.323942591573772	23.859984767304887
22	25.173252134002027	25.512362879062618	25.744742544313638	23.56964244262172
23	23.671855573096995	25.484213943874558	26.504398715537057	24.33953176749139
24	24.097033481755624	25.73543382541243	26.666942032696962	23.500590660134986
25	24.066220022965528	25.628371512833432	26.647611337370197	23.65779712683084
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	214.0
1	214.0
2	527.5
3	841.0
4	841.0
5	841.0
6	2415.0
7	3989.0
8	3989.0
9	3989.0
10	3821.0
11	3653.0
12	3653.0
13	3653.0
14	3560.0
15	3467.0
16	3467.0
17	3467.0
18	6365.5
19	9264.0
20	9264.0
21	9264.0
22	15365.0
23	21466.0
24	21466.0
25	21466.0
26	32846.5
27	44227.0
28	44227.0
29	44227.0
30	56910.0
31	69593.0
32	69593.0
33	69593.0
34	79787.0
35	89981.0
36	89981.0
37	89981.0
38	98424.5
39	106868.0
40	106868.0
41	106868.0
42	119132.5
43	131397.0
44	131397.0
45	131397.0
46	146343.5
47	161290.0
48	161290.0
49	161290.0
50	160556.5
51	159823.0
52	159823.0
53	159823.0
54	143731.0
55	127639.0
56	127639.0
57	127639.0
58	115440.5
59	103242.0
60	103242.0
61	103242.0
62	89752.0
63	76262.0
64	76262.0
65	76262.0
66	61589.0
67	46916.0
68	46916.0
69	46916.0
70	35282.0
71	23648.0
72	23648.0
73	23648.0
74	18176.5
75	12705.0
76	12705.0
77	12705.0
78	10486.0
79	8267.0
80	8267.0
81	8267.0
82	5704.0
83	3141.0
84	3141.0
85	3141.0
86	2473.0
87	1805.0
88	1805.0
89	1805.0
90	1279.0
91	753.0
92	753.0
93	753.0
94	528.0
95	303.0
96	303.0
97	303.0
98	516.0
99	729.0
100	729.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03656675330978644
2	0.004374803443383027
3	0.002641390758268998
4	0.0044573469045789335
5	0.00907978073154968
6	0.013784758019716332
7	0.024515407975184134
8	0.03648420984859053
9	0.04969116363993552
10	0.05868840091028929
11	0.056377183996803915
12	0.06611731241792085
13	0.06512679088356997
14	0.06809835548662259
15	0.061494878590950104
16	0.06611731241792085
17	0.06570459511194131
18	0.07915917928687402
19	0.0761876146838214
20	0.08122276581677167
21	0.07668287545099683
22	0.07957189659285356
23	0.08386415657504066
24	0.08031478774361671
25	0.08031478774361671
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1211483.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.817795416036994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.55834797582288	36.36437487744952
2	16.215049017023674	16.480260872162418
3	5.867991128814689	8.945951180616744
4	2.525085198259214	5.132770520527998
5	1.281303851143623	3.2556518486598476
6	0.7120883777119504	2.1712056898022145
7	0.42574072193143536	1.5144643435171306
8	0.2770134767360499	1.126177135060617
9	0.19323077523326537	0.8837605803497677
>10	0.7674090478522112	7.004739202171878
>50	0.0799212677513907	2.8469521012179957
>100	0.08573549274917158	8.759083274460512
>500	0.0091277273874422	3.2206996020603187
>1k	0.0017929464511047177	1.8123320549618027
>5k	1.629951319186107E-4	0.48157671698116644
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5814	0.4799076833929985	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4059	0.3350439089941831	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3158	0.2606722504566717	No Hit
GTACATGGGGTGGTATCAACGCAAA	2513	0.2074317179853122	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2105	0.1737539858173825	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2010	0.1659123570037714	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1965	0.16219790124995562	No Hit
GTATCAACGCAGAGTACATGGGGTG	1510	0.12464062640581833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6508692239181234E-4
2	0.0	0.0	0.0	0.0	1.6508692239181234E-4
3	0.0	0.0	0.0	0.0	1.6508692239181234E-4
4	0.0	0.0	0.0	0.0	1.6508692239181234E-4
5	0.0	0.0	0.0	0.0	1.6508692239181234E-4
6	0.0	0.0	0.0	0.0	2.4763038358771854E-4
7	0.0	0.0	0.0	0.0	2.4763038358771854E-4
8	0.0	0.0	0.0	0.0	2.4763038358771854E-4
9	0.0	0.0	0.0	0.0	2.4763038358771854E-4
10	0.0	0.0	0.0	0.0	2.4763038358771854E-4
11	0.0	0.0	0.0	0.0	2.4763038358771854E-4
12	0.0	0.0	0.0	8.254346119590617E-5	6.603476895672494E-4
13	0.0	0.0	0.0	8.254346119590617E-5	6.603476895672494E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAG	150	0.0	15.835571	17
AACCGTG	50	8.7101085E-5	15.200265	7
CGCCAGT	165	0.0	14.395973	18
GTATACG	40	0.005286286	14.2467165	1
CGTTATT	100	6.6211214E-10	14.244951	2
ATAATAC	75	9.697287E-7	13.929548	3
AACCGCG	70	7.258068E-6	13.571665	7
GCATCGC	175	0.0	13.030413	14
CGAGCCG	230	0.0	12.806158	15
TCGTTCA	60	4.08599E-4	12.668457	16
CGTTTCC	75	1.4757976E-5	12.668457	14
ATCGCCA	180	0.0	12.668456	16
CGCATCG	180	0.0	12.667933	13
GCTAGAC	60	4.1002542E-4	12.663225	3
CCTATAC	100	1.4464968E-7	12.346645	3
TTAGGAC	340	0.0	12.290778	3
CAACGGA	55	0.0030645258	12.092619	14
GTACTAA	55	0.0030730711	12.088122	1
AATCGAA	80	2.8646231E-5	11.875207	7
GTAATAG	80	2.8731125E-5	11.871774	3
>>END_MODULE