FastQCFastQC Report
Thu 2 Feb 2017
SRR4064005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211483
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16230.1339680375209557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.11003043377414293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTT301.3018624E-436.6631935
GGCGTTA2050.021.46226742
TAGGACC8550.020.840134
GTAGGAC9100.019.3388273
TAGGACG6450.018.7579124
TGTAGGA9500.018.5245632
GATTTCG3450.018.49176441
ATACACT4900.017.9574814
TTAGGAC5300.017.8473663
CGCGGTT3950.017.82183310
ATTTCGT4050.017.38178642
CTGTAGG9750.017.3741471
CTTACAC1802.8558134E-1017.109493
AGGACGT6700.017.0730085
AATCACG4850.016.78468734
ATCACGG4850.016.78468735
GCGAGAA6050.016.7270720
TATGGCG9650.016.64159816
GTCCTAA4500.016.6220191
ATACCGT2251.8189894E-1216.6206476
Produced by FastQC (version 0.11.5)