##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064005_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1211483
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204196014306433	32.0	32.0	32.0	32.0	32.0
2	31.417141635499632	32.0	32.0	32.0	32.0	32.0
3	31.495420076055545	32.0	32.0	32.0	32.0	32.0
4	31.57847200497242	32.0	32.0	32.0	32.0	32.0
5	31.54222552029207	32.0	32.0	32.0	32.0	32.0
6	35.11061071430635	36.0	36.0	36.0	36.0	36.0
7	35.1372631724919	36.0	36.0	36.0	36.0	36.0
8	35.08426614323106	36.0	36.0	36.0	36.0	36.0
9	35.19151568779752	36.0	36.0	36.0	36.0	36.0
10	35.043525992523215	36.0	36.0	36.0	36.0	36.0
11	35.192058823772186	36.0	36.0	36.0	36.0	36.0
12	35.1064109030007	36.0	36.0	36.0	36.0	36.0
13	35.14458395206536	36.0	36.0	36.0	36.0	36.0
14	35.08467225706015	36.0	36.0	36.0	36.0	36.0
15	35.06536534148643	36.0	36.0	36.0	36.0	36.0
16	35.07559330176321	36.0	36.0	36.0	36.0	36.0
17	35.04299276176389	36.0	36.0	36.0	36.0	36.0
18	35.031056151840346	36.0	36.0	36.0	36.0	36.0
19	35.03151344261537	36.0	36.0	36.0	36.0	36.0
20	35.01914513038978	36.0	36.0	36.0	36.0	36.0
21	35.02063834160281	36.0	36.0	36.0	36.0	36.0
22	34.99823191906118	36.0	36.0	36.0	36.0	36.0
23	34.955313446412376	36.0	36.0	36.0	36.0	36.0
24	34.9324621146149	36.0	36.0	36.0	36.0	36.0
25	34.898997344576856	36.0	36.0	36.0	32.0	36.0
26	34.845051065512266	36.0	36.0	36.0	32.0	36.0
27	34.832282417499876	36.0	36.0	36.0	32.0	36.0
28	34.81034154007939	36.0	36.0	36.0	32.0	36.0
29	34.77507402084883	36.0	36.0	36.0	32.0	36.0
30	34.7475738413168	36.0	36.0	36.0	32.0	36.0
31	34.73268547722089	36.0	36.0	36.0	32.0	36.0
32	34.6849365612229	36.0	36.0	36.0	32.0	36.0
33	34.64880481195362	36.0	36.0	36.0	32.0	36.0
34	34.62740624507319	36.0	36.0	36.0	32.0	36.0
35	34.57340218558576	36.0	36.0	36.0	32.0	36.0
36	34.54348678437915	36.0	36.0	36.0	32.0	36.0
37	34.527988424105004	36.0	36.0	36.0	32.0	36.0
38	34.47923412874964	36.0	36.0	36.0	32.0	36.0
39	34.44867819028414	36.0	36.0	36.0	32.0	36.0
40	34.43813986659326	36.0	36.0	36.0	32.0	36.0
41	34.4071868940794	36.0	36.0	36.0	32.0	36.0
42	34.340489301129274	36.0	36.0	36.0	32.0	36.0
43	34.316104311822784	36.0	36.0	36.0	32.0	36.0
44	34.23769215085973	36.0	36.0	36.0	32.0	36.0
45	34.17675691693569	36.0	36.0	36.0	32.0	36.0
46	34.12343796817619	36.0	36.0	36.0	32.0	36.0
47	34.05638791464676	36.0	36.0	36.0	32.0	36.0
48	33.994840208240646	36.0	36.0	36.0	32.0	36.0
49	33.95975015745165	36.0	36.0	36.0	32.0	36.0
50	33.39272032707021	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	3.0
20	15.0
21	52.0
22	149.0
23	479.0
24	1132.0
25	2685.0
26	5340.0
27	9405.0
28	15454.0
29	22705.0
30	31990.0
31	44108.0
32	62136.0
33	98425.0
34	199927.0
35	717474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.91907007691298	18.297975266000698	12.140396526574479	25.642558130511844
2	15.194828135550129	20.889359938851552	38.69182931922825	25.223982606370072
3	18.054393063679242	25.754326533374932	29.138292234774383	27.05298816817144
4	11.559551392797092	16.47872896276712	36.94719612243837	35.01452352199742
5	13.099399661406721	38.33598985705949	33.814754313514925	14.749856168018866
6	32.789812155845354	36.86770676930671	17.352121325681004	12.99035974916693
7	29.13538954767797	31.23628745619002	21.26742287545337	18.36090012067864
8	27.67096195324243	33.92222589999199	19.473818452260577	18.932993694504997
9	27.714979561918472	13.880148316417163	18.81929632246604	39.585575799198324
10	15.577604067785417	27.73828489355906	32.22498287204801	24.45912816660751
11	36.518412985087686	21.172250186135656	23.369393849846716	18.93994297892994
12	24.863122079504343	24.245035621689485	29.82539552828315	21.06644677052302
13	30.070021849326316	20.189702008866846	25.597389636964408	24.14288650484243
14	23.167365536368738	20.44301185327038	26.536302704130488	29.853319906230396
15	24.431799889880658	28.67040390023881	23.252680960168	23.645115249712525
16	24.238034391219937	26.28323229921946	25.606865036913774	23.87186827264683
17	22.663050162486805	26.753491382049933	26.44288033756974	24.140578117893526
18	23.31839564432507	25.463710825711093	28.299588023544814	22.918305506419024
19	24.880146597550105	25.01130848218708	26.856819757651795	23.25172516261102
20	25.370209562550144	24.647207208397028	26.55397217939109	23.428611049661736
21	26.172467958691954	24.339507859375658	25.590866731105592	23.897157450826796
22	25.100166572842188	25.287210210304405	26.06146851542161	23.551154701431802
23	23.64885779731319	25.288016673889267	26.810788501619925	24.252337027177614
24	24.1981881590623	25.41761076373842	26.869972554117915	23.514228523081368
25	24.123434431649056	25.267507579690967	26.88596661386541	23.723091374794567
26	23.486069195057684	26.229140113718163	27.272216975836542	23.012573715387614
27	23.96650825530397	25.70944598948492	26.61046736717674	23.71357838803437
28	23.30543242283912	25.909132182627143	26.65544026481101	24.129995129722726
29	23.434843046810254	25.532339918312363	26.770696788295623	24.262120246581766
30	23.227348745371188	25.936121984359033	27.031022738341505	23.805506531928277
31	24.38194864073278	25.332346036117247	26.144719941092365	24.140985382057607
32	23.547110565207916	26.124852242935724	26.121880592481062	24.20615659937529
33	23.279881264094936	25.624964917436284	26.90023674539469	24.19491707307409
34	23.98433275963828	25.836831222218095	26.92850273846522	23.2503332796784
35	24.332431658286893	25.97657846100001	26.59724016446421	23.093749716248887
36	23.258325030110363	26.34984814801668	26.547391934963272	23.844434886909692
37	23.9402589340844	26.254387384144994	26.067335409120474	23.73801827265013
38	23.622412547724693	26.31629346816634	26.2784851924466	23.782808791662365
39	24.200920403656852	25.810842602719937	26.127577027054915	23.8606599665683
40	24.811012221677423	25.932722166916776	26.41932751883274	22.836938092573057
41	23.73785025321001	25.9736100276942	27.068913570157953	23.219626148937838
42	24.464405699578524	26.875384866829886	26.077173125275493	22.583036308316096
43	23.452145209072967	26.15027895931131	26.530974430174325	23.866601401441393
44	23.33906747076663	26.60546217863104	26.018813693912623	24.036656656689708
45	23.265943905978375	26.794148577013306	26.02384218789467	23.916065329113653
46	23.49812252417936	25.929389739877358	25.986675658519793	24.585812077423487
47	24.203873181015616	25.965963354929883	26.1626683197698	23.6674951442847
48	24.363265659888317	26.45760676211663	25.27043926518113	23.90868831281393
49	23.73967579989335	26.620463996751063	25.516767177834605	24.123093025520976
50	23.621118458469084	27.102836138875798	25.298913469657965	23.97713193299716
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	33.0
2	30.0
3	92.5
4	155.0
5	199.5
6	244.0
7	260.0
8	276.0
9	400.0
10	524.0
11	768.5
12	1013.0
13	1921.0
14	2829.0
15	4042.0
16	5255.0
17	6069.5
18	6884.0
19	7269.5
20	7655.0
21	7951.0
22	8247.0
23	8979.0
24	9711.0
25	9840.5
26	9970.0
27	12557.5
28	15145.0
29	17862.5
30	20580.0
31	24112.0
32	27644.0
33	32869.5
34	38095.0
35	43690.5
36	49286.0
37	53687.0
38	58088.0
39	59132.5
40	60177.0
41	62543.5
42	64910.0
43	65888.0
44	66866.0
45	72401.5
46	77937.0
47	81709.5
48	85482.0
49	86972.5
50	88463.0
51	85510.0
52	82557.0
53	80065.0
54	77573.0
55	77437.5
56	77302.0
57	73487.0
58	69672.0
59	62484.5
60	55297.0
61	48422.0
62	41547.0
63	36031.0
64	30515.0
65	25788.0
66	21061.0
67	18188.5
68	15316.0
69	14259.0
70	13202.0
71	10387.5
72	7573.0
73	6408.0
74	5243.0
75	4134.0
76	3025.0
77	2688.5
78	2352.0
79	1958.0
80	1564.0
81	1270.5
82	977.0
83	801.5
84	626.0
85	446.5
86	267.0
87	187.0
88	107.0
89	82.5
90	58.0
91	46.5
92	35.0
93	36.0
94	37.0
95	33.5
96	30.0
97	27.0
98	24.0
99	22.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009409954576333304
2	4.127173059795309E-4
3	4.127173059795309E-4
4	0.0
5	0.0
6	0.0
7	8.254346119590618E-5
8	0.0
9	0.0015683257627222174
10	0.0010730649955467803
11	8.254346119590618E-5
12	1.6508692239181237E-4
13	3.3017384478362474E-4
14	2.4763038358771854E-4
15	0.005778042283713433
16	9.079780731549679E-4
17	0.0
18	4.952607671754371E-4
19	2.4763038358771854E-4
20	5.778042283713432E-4
21	0.0
22	8.254346119590618E-5
23	6.603476895672495E-4
24	6.603476895672495E-4
25	0.0018159561463099358
26	0.005530411900125713
27	0.006603476895672494
28	0.004374803443383027
29	0.0032191949866403405
30	0.004044629598599403
31	0.007841628813611086
32	0.0028890211418567163
33	0.00453989036577484
34	0.0031366515254444348
35	0.00280647768066081
36	0.008749606886766055
37	0.00453989036577484
38	0.008914693809157867
39	0.004787520749362558
40	0.002393760374681279
41	0.0031366515254444348
42	0.0020635865298976545
43	0.001320695379134499
44	0.0020635865298976545
45	0.001403238840330405
46	0.001320695379134499
47	0.0019810430687017483
48	0.002311216913485373
49	7.428911507631556E-4
50	9.905215343508742E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1211483.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.30693320352134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14581541179523	40.4547072709726
2	15.457460756457875	17.09809499106936
3	5.515685929705877	9.151670198575363
4	2.4064458949206826	5.323725694730652
5	1.1991680253538184	3.316115293902111
6	0.6588474463291505	2.1863299023262157
7	0.40018068501532794	1.549293649083751
8	0.2575220206674429	1.1394202556392083
9	0.16615448709707967	0.8270545607409179
>10	0.6540786346006489	6.336751651354476
>50	0.06635527884150312	2.5950073131653344
>100	0.06583673695554393	7.3463181728894416
>500	0.005548874735223899	2.0786490471075303
>1k	8.998175246309025E-4	0.5968619984432301
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1623	0.1339680375209557	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1333	0.11003043377414293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4763038358771854E-4
2	0.0	0.0	0.0	0.0	2.4763038358771854E-4
3	0.0	0.0	0.0	0.0	2.4763038358771854E-4
4	0.0	0.0	0.0	0.0	2.4763038358771854E-4
5	0.0	0.0	0.0	0.0	3.301738447836247E-4
6	0.0	0.0	0.0	0.0	4.952607671754371E-4
7	0.0	0.0	0.0	0.0	5.778042283713432E-4
8	0.0	0.0	0.0	0.0	5.778042283713432E-4
9	0.0	0.0	0.0	0.0	5.778042283713432E-4
10	0.0	0.0	0.0	0.0	5.778042283713432E-4
11	0.0	0.0	0.0	0.0	5.778042283713432E-4
12	0.0	0.0	0.0	8.254346119590617E-5	9.905215343508742E-4
13	0.0	0.0	0.0	8.254346119590617E-5	0.0010730649955467803
14	0.0	0.0	0.0	8.254346119590617E-5	0.0010730649955467803
15	0.0	0.0	0.0	8.254346119590617E-5	0.0013206953791344987
16	0.0	0.0	0.0	1.6508692239181234E-4	0.0014032388403304049
17	0.0	0.0	0.0	1.6508692239181234E-4	0.0014032388403304049
18	0.0	0.0	0.0	1.6508692239181234E-4	0.0014857823015263112
19	0.0	0.0	0.0	2.4763038358771854E-4	0.0014857823015263112
20	0.0	0.0	0.0	3.301738447836247E-4	0.0017334126851140297
21	0.0	0.0	0.0	4.952607671754371E-4	0.001898499607505842
22	0.0	0.0	0.0	9.905215343508742E-4	0.001898499607505842
23	0.0	0.0	0.0	0.0029715646030526225	0.001898499607505842
24	8.254346119590617E-5	0.0	0.0	0.005447868438929807	0.001898499607505842
25	8.254346119590617E-5	0.0	0.0	0.006355846512084776	0.001898499607505842
26	8.254346119590617E-5	0.0	0.0	0.007511454968827462	0.0019810430687017483
27	8.254346119590617E-5	0.0	0.0	0.00883215034796196	0.0019810430687017483
28	8.254346119590617E-5	0.0	0.0	0.01229897571819002	0.0019810430687017483
29	8.254346119590617E-5	0.0	0.0	0.018984996075058422	0.0019810430687017483
30	8.254346119590617E-5	0.0	0.0	0.031861776021619784	0.0019810430687017483
31	8.254346119590617E-5	0.0	0.0	0.061990139358125536	0.0019810430687017483
32	1.6508692239181234E-4	0.0	0.0	0.09071526385430088	0.0020635865298976545
33	1.6508692239181234E-4	0.0	0.0	0.1203483664236312	0.0020635865298976545
34	1.6508692239181234E-4	0.0	0.0	0.15031164283774515	0.0021461299910935606
35	1.6508692239181234E-4	0.0	0.0	0.1884467219102538	0.0021461299910935606
36	1.6508692239181234E-4	0.0	0.0	0.23978875477410744	0.0021461299910935606
37	1.6508692239181234E-4	0.0	0.0	0.3195257382893528	0.0021461299910935606
38	1.6508692239181234E-4	0.0	0.0	0.4224574344006478	0.002311216913485373
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTT	30	1.3018624E-4	36.663193	5
GGCGTTA	205	0.0	21.462267	42
TAGGACC	855	0.0	20.84013	4
GTAGGAC	910	0.0	19.338827	3
TAGGACG	645	0.0	18.757912	4
TGTAGGA	950	0.0	18.524563	2
GATTTCG	345	0.0	18.491764	41
ATACACT	490	0.0	17.957481	4
TTAGGAC	530	0.0	17.847366	3
CGCGGTT	395	0.0	17.821833	10
ATTTCGT	405	0.0	17.381786	42
CTGTAGG	975	0.0	17.374147	1
CTTACAC	180	2.8558134E-10	17.10949	3
AGGACGT	670	0.0	17.073008	5
AATCACG	485	0.0	16.784687	34
ATCACGG	485	0.0	16.784687	35
GCGAGAA	605	0.0	16.72707	20
TATGGCG	965	0.0	16.641598	16
GTCCTAA	450	0.0	16.622019	1
ATACCGT	225	1.8189894E-12	16.620647	6
>>END_MODULE