FastQCFastQC Report
Thu 2 Feb 2017
SRR4064004_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064004_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956293
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57050.1929781655607208No Hit
TATCAACGCAGAGTACTTTTTTTTT39890.13493249823342948No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG32580.11020558517034677No Hit
GATTAAGAGGGACGGCCGGGGGCAT32100.10858193014021277No Hit
GTATCTGATCGTCTTCGAACCTCCG31660.10709357969592324No Hit
GAATAGGACCGCGGTTCTATTTTGT30830.10428600953964982No Hit
GCGCAAGACGGACCAGAGCGAAAGC29750.10063278572184828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2650.013.2612091
CGAACGA2400.013.06535216
CGTCGTA4900.012.99065210
GGTATCA15550.012.3380991
CGTTATT2700.012.3070832
CCGTCGT5200.012.2409849
GTATCAA39050.011.9909371
TATACCG1201.0055373E-811.8727675
CCGACCA5200.011.692889
CGGCGTC3450.011.56715614
ATACCGT6350.011.5186896
ACCGTCG5900.011.4327648
CGTCTTA2350.011.32142915
CGACCAT5650.011.2662310
CGCATCG4700.011.11888313
ATACCGA600.00588156411.0823766
CGTCAAT4850.010.97128219
TACCGTC6250.010.9443517
CGGTCCA6200.010.87974910
GTCGTAG5650.010.76231911
Produced by FastQC (version 0.11.5)