##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064004_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2956293
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240976452604663	32.0	32.0	32.0	32.0	32.0
2	30.761102840618303	32.0	32.0	32.0	32.0	32.0
3	30.774725982844053	32.0	32.0	32.0	32.0	32.0
4	30.823464724234032	32.0	32.0	32.0	32.0	32.0
5	30.678929321281753	32.0	32.0	32.0	32.0	32.0
6	34.35765500916181	36.0	36.0	36.0	32.0	36.0
7	34.29866829843997	36.0	36.0	36.0	32.0	36.0
8	34.24503017799657	36.0	36.0	36.0	32.0	36.0
9	34.428189628024015	36.0	36.0	36.0	32.0	36.0
10	34.05133997205284	36.0	36.0	36.0	32.0	36.0
11	34.402760484160396	36.0	36.0	36.0	32.0	36.0
12	34.17170896118889	36.0	36.0	36.0	32.0	36.0
13	34.28484152281252	36.0	36.0	36.0	32.0	36.0
14	34.15922914271353	36.0	36.0	36.0	32.0	36.0
15	34.076372335218466	36.0	36.0	36.0	32.0	36.0
16	34.08297925814525	36.0	36.0	36.0	32.0	36.0
17	33.993948502398105	36.0	36.0	36.0	32.0	36.0
18	33.99586373881073	36.0	36.0	36.0	32.0	36.0
19	34.01722089116336	36.0	36.0	36.0	32.0	36.0
20	34.00275649267512	36.0	36.0	36.0	32.0	36.0
21	33.98735341862258	36.0	36.0	36.0	32.0	36.0
22	33.97130122081945	36.0	36.0	36.0	32.0	36.0
23	33.900371850828044	36.0	36.0	36.0	32.0	36.0
24	33.87992191572351	36.0	36.0	36.0	32.0	36.0
25	33.46230126716127	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	23.0
5	34.0
6	111.0
7	49.0
8	183.0
9	198.0
10	264.0
11	101.0
12	193.0
13	162.0
14	247.0
15	449.0
16	763.0
17	1025.0
18	1421.0
19	2015.0
20	3169.0
21	4617.0
22	7441.0
23	11381.0
24	16966.0
25	25001.0
26	35422.0
27	47452.0
28	63549.0
29	85837.0
30	112900.0
31	152454.0
32	215935.0
33	302766.0
34	629761.0
35	1234393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24534256388842	17.26756303396858	11.508392403268504	25.97870199887449
2	16.98003936243411	19.512617561274617	36.89274244126926	26.61460063502201
3	18.57980743828239	23.42818445143454	28.66722570881256	29.32478240147051
4	12.714022198264855	15.444406745604205	35.63003839080001	36.21153266533093
5	14.981615501638517	36.21463703090267	33.34094491145705	15.462802556001765
6	34.563657806011946	35.29141916848731	16.69720431649906	13.447718709001682
7	30.696620211334487	30.316460385864254	20.578231350057973	18.408688052743287
8	28.542994681286643	32.37413991349562	19.180360486812177	19.902504918405565
9	27.37307142100513	14.126290133005915	18.503731685500796	39.996906760488166
10	16.239489519727364	26.346033627444577	31.036763284844298	26.37771356798376
11	37.234027430864394	20.973909405854137	22.389688312708063	19.402374850573405
12	24.879652527347698	23.564478557468586	28.371032392103608	23.18483652308011
13	29.312649463581238	19.274315704317242	25.238270123531176	26.174764708570347
14	23.89384185545221	19.622176171555807	24.8640764407965	31.619905532195485
15	25.313854194837194	27.06634710644219	21.938265215806386	25.681533482914237
16	26.11801971779306	25.62785051955024	23.23596970638591	25.018160056270787
17	24.35152136717421	25.823014031542623	24.77641089691191	25.049053704371254
18	25.174554587472286	24.80071578806255	25.63526021286096	24.389469411604203
19	25.78540899221615	24.798599625059158	25.039724907974296	24.376266474750395
20	26.02194088027575	24.11980210823653	24.464833046597455	25.39342396489026
21	27.22012433840493	23.990744862535482	24.096566222133458	24.692564576926138
22	26.332129748804483	23.888455947519834	24.643647125175274	25.135767178500412
23	24.728811551026627	23.931479255886384	25.25575096907426	26.083958224012726
24	25.03595115517629	24.803995114434354	24.849763440044846	25.310290290344504
25	25.103640466600453	24.247671432469343	24.972528421150983	25.676159679779225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	131.0
1	131.0
2	389.5
3	648.0
4	648.0
5	648.0
6	2047.0
7	3446.0
8	3446.0
9	3446.0
10	3835.5
11	4225.0
12	4225.0
13	4225.0
14	5650.0
15	7075.0
16	7075.0
17	7075.0
18	12207.0
19	17339.0
20	17339.0
21	17339.0
22	28294.5
23	39250.0
24	39250.0
25	39250.0
26	59013.0
27	78776.0
28	78776.0
29	78776.0
30	98181.5
31	117587.0
32	117587.0
33	117587.0
34	148699.5
35	179812.0
36	179812.0
37	179812.0
38	207732.5
39	235653.0
40	235653.0
41	235653.0
42	271063.5
43	306474.0
44	306474.0
45	306474.0
46	343480.0
47	380486.0
48	380486.0
49	380486.0
50	391600.0
51	402714.0
52	402714.0
53	402714.0
54	377143.0
55	351572.0
56	351572.0
57	351572.0
58	324448.5
59	297325.0
60	297325.0
61	297325.0
62	262497.0
63	227669.0
64	227669.0
65	227669.0
66	187068.0
67	146467.0
68	146467.0
69	146467.0
70	110729.0
71	74991.0
72	74991.0
73	74991.0
74	57835.5
75	40680.0
76	40680.0
77	40680.0
78	32878.5
79	25077.0
80	25077.0
81	25077.0
82	17202.0
83	9327.0
84	9327.0
85	9327.0
86	7081.0
87	4835.0
88	4835.0
89	4835.0
90	3514.0
91	2193.0
92	2193.0
93	2193.0
94	1523.0
95	853.0
96	853.0
97	853.0
98	1270.5
99	1688.0
100	1688.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03991485282412806
2	0.005919575630696957
3	0.0015560027372117716
4	0.003923832989490555
5	0.009133059544503877
6	0.012820109508766555
7	0.023475345644021078
8	0.036092498274020876
9	0.04945382612616543
10	0.05892514713528057
11	0.056320533857773906
12	0.06531828881643328
13	0.06690811770010618
14	0.06964803556345735
15	0.06142828197340385
16	0.06788907594747882
17	0.06352550305399363
18	0.07935613959780036
19	0.07533082816892642
20	0.07966057491595048
21	0.07637943870922131
22	0.07911935657257248
23	0.08263727580452952
24	0.0772927446636717
25	0.07884874740088348
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2956293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.18568177806257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.02618235349993	35.87347247333204
2	13.370581871014576	12.618000427064525
3	4.297378847255059	6.083242522990639
4	1.9310823656101535	3.644777519636359
5	1.0600845018935208	2.501040498210182
6	0.6610539485194417	1.871536875178208
7	0.47585274841814124	1.5717405452052418
8	0.33838641477768033	1.277359494857529
9	0.2539517804011975	1.0784599107285
>10	1.3893741212323838	11.746451848946899
>50	0.09792649156898177	3.209472739355275
>100	0.07697068774147303	7.785748759939144
>500	0.01339571245087659	4.40217192242562
>1k	0.007706135657185571	6.142650826053286
>5k	7.201995941294928E-5	0.1938736360764456
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5705	0.1929781655607208	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3989	0.13493249823342948	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3258	0.11020558517034677	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3210	0.10858193014021277	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3166	0.10709357969592324	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3083	0.10428600953964982	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2975	0.10063278572184828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.0147843938337641E-4	0.0	0.0	0.0
6	0.0	1.0147843938337641E-4	0.0	0.0	3.382614646112547E-5
7	0.0	1.0147843938337641E-4	0.0	0.0	3.382614646112547E-5
8	0.0	1.0147843938337641E-4	0.0	0.0	3.382614646112547E-5
9	0.0	1.0147843938337641E-4	0.0	0.0	3.382614646112547E-5
10	0.0	1.0147843938337641E-4	0.0	3.382614646112547E-5	3.382614646112547E-5
11	0.0	1.0147843938337641E-4	0.0	3.382614646112547E-5	3.382614646112547E-5
12	0.0	1.0147843938337641E-4	0.0	3.382614646112547E-5	2.0295687876675282E-4
13	0.0	1.0147843938337641E-4	0.0	3.382614646112547E-5	2.0295687876675282E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	265	0.0	13.261209	1
CGAACGA	240	0.0	13.065352	16
CGTCGTA	490	0.0	12.990652	10
GGTATCA	1555	0.0	12.338099	1
CGTTATT	270	0.0	12.307083	2
CCGTCGT	520	0.0	12.240984	9
GTATCAA	3905	0.0	11.990937	1
TATACCG	120	1.0055373E-8	11.872767	5
CCGACCA	520	0.0	11.69288	9
CGGCGTC	345	0.0	11.567156	14
ATACCGT	635	0.0	11.518689	6
ACCGTCG	590	0.0	11.432764	8
CGTCTTA	235	0.0	11.321429	15
CGACCAT	565	0.0	11.26623	10
CGCATCG	470	0.0	11.118883	13
ATACCGA	60	0.005881564	11.082376	6
CGTCAAT	485	0.0	10.971282	19
TACCGTC	625	0.0	10.944351	7
CGGTCCA	620	0.0	10.879749	10
GTCGTAG	565	0.0	10.762319	11
>>END_MODULE