FastQCFastQC Report
Thu 2 Feb 2017
SRR4064004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956293
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA32940.11142332644294729No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT32710.11064532507434141No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA32690.11057767278141917No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT29580.10005774123200914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46250.023.878121
GGTATCA22250.021.1588421
AACGCAG62800.017.23486
TAGCGCA1451.2517376E-716.6888169
ACCGTCG8650.016.530968
CGAGCCG10400.016.49916815
GGCGTTA9000.016.13224642
TCAACGC67250.016.0289334
ATACCGT9900.015.7769826
CAACGCA68600.015.74556355
TATCAAC69600.015.7400542
TAAACGC7750.015.61449328
TCTATAC3100.015.6118533
ATCAACG69050.015.6108263
TACCGTC9650.015.5018117
CGTCGTA9150.015.38746510
CGCGGGA7150.015.3838444
CCGTCGT9250.015.2208559
GTATTAC2800.014.9280731
GTAAACG7550.014.8624427
Produced by FastQC (version 0.11.5)