##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064004_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2956293 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.360008970694043 32.0 32.0 32.0 32.0 32.0 2 31.421694669642015 32.0 32.0 32.0 32.0 32.0 3 31.48881623032629 32.0 32.0 32.0 32.0 32.0 4 31.569005846172892 32.0 32.0 32.0 32.0 32.0 5 31.52330807534977 32.0 32.0 32.0 32.0 32.0 6 35.11613598516791 36.0 36.0 36.0 36.0 36.0 7 35.129378244984515 36.0 36.0 36.0 36.0 36.0 8 35.07829839599796 36.0 36.0 36.0 36.0 36.0 9 35.182026612382465 36.0 36.0 36.0 36.0 36.0 10 35.044862941528464 36.0 36.0 36.0 36.0 36.0 11 35.179558994998125 36.0 36.0 36.0 36.0 36.0 12 35.08559571057402 36.0 36.0 36.0 36.0 36.0 13 35.13539659296288 36.0 36.0 36.0 36.0 36.0 14 35.073069888539465 36.0 36.0 36.0 36.0 36.0 15 35.04548500436188 36.0 36.0 36.0 36.0 36.0 16 35.056854310448934 36.0 36.0 36.0 36.0 36.0 17 35.016383694038446 36.0 36.0 36.0 36.0 36.0 18 35.01790248801455 36.0 36.0 36.0 36.0 36.0 19 35.01696313592733 36.0 36.0 36.0 36.0 36.0 20 35.01142511922871 36.0 36.0 36.0 36.0 36.0 21 35.00463925598714 36.0 36.0 36.0 36.0 36.0 22 34.99356491389724 36.0 36.0 36.0 36.0 36.0 23 34.940199432194305 36.0 36.0 36.0 36.0 36.0 24 34.90449018415969 36.0 36.0 36.0 32.0 36.0 25 34.881037840295264 36.0 36.0 36.0 32.0 36.0 26 34.82094095544657 36.0 36.0 36.0 32.0 36.0 27 34.82293669808777 36.0 36.0 36.0 32.0 36.0 28 34.798552782149805 36.0 36.0 36.0 32.0 36.0 29 34.7700170449952 36.0 36.0 36.0 32.0 36.0 30 34.75767692850472 36.0 36.0 36.0 32.0 36.0 31 34.74631032850939 36.0 36.0 36.0 32.0 36.0 32 34.7173490584323 36.0 36.0 36.0 32.0 36.0 33 34.68991842148258 36.0 36.0 36.0 32.0 36.0 34 34.6828162837716 36.0 36.0 36.0 32.0 36.0 35 34.6452401707138 36.0 36.0 36.0 32.0 36.0 36 34.618917340060676 36.0 36.0 36.0 32.0 36.0 37 34.61500162534634 36.0 36.0 36.0 32.0 36.0 38 34.57591855746369 36.0 36.0 36.0 32.0 36.0 39 34.583990829055175 36.0 36.0 36.0 32.0 36.0 40 34.55902510339807 36.0 36.0 36.0 32.0 36.0 41 34.53818278499459 36.0 36.0 36.0 32.0 36.0 42 34.47371894463776 36.0 36.0 36.0 32.0 36.0 43 34.474596056615496 36.0 36.0 36.0 32.0 36.0 44 34.41287754630546 36.0 36.0 36.0 32.0 36.0 45 34.388060317431325 36.0 36.0 36.0 32.0 36.0 46 34.36677183215601 36.0 36.0 36.0 32.0 36.0 47 34.338854098697254 36.0 36.0 36.0 32.0 36.0 48 34.29739169967253 36.0 36.0 36.0 32.0 36.0 49 34.28457463451694 36.0 36.0 36.0 32.0 36.0 50 33.6819665709725 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 4.0 18 2.0 19 11.0 20 25.0 21 101.0 22 332.0 23 872.0 24 2231.0 25 5124.0 26 10726.0 27 19391.0 28 32362.0 29 49674.0 30 72485.0 31 103182.0 32 149456.0 33 241908.0 34 509561.0 35 1758846.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33181327967589 18.170820653063764 12.076848861109946 26.4205172061504 2 16.206758563721486 20.233983328366502 36.469900168421184 27.089357939490828 3 17.75879297035939 24.006573121238095 29.011609853187213 29.2230240552153 4 12.30003250692675 16.085922471148834 35.74310800722391 35.8709370147005 5 14.519535106973496 36.68462496782288 33.146139438817464 15.649700486386159 6 34.56307369241427 35.60782981901628 16.499238233893315 13.329858254676136 7 30.53953791391843 30.528375270482687 20.64385450813503 18.28823230746385 8 28.48798427218962 32.592856186060104 19.07446896314708 19.844690578603196 9 27.427336275118353 13.996864275916657 18.396349435349794 40.179450013615195 10 16.139000187737526 26.544022758523877 31.108141888977777 26.208835164760817 11 37.37931664349573 20.938101471777127 22.361608637853525 19.320973246873617 12 24.802066103122495 23.571774081703754 28.410440112438224 23.215719702735523 13 29.379317296721517 19.245760799816797 25.31484701873336 26.060074884728323 14 23.85022964625209 19.61055188841675 24.858724764789333 31.680493700541827 15 25.344349914584846 27.124266359961435 21.926762850328977 25.604620875124738 16 26.16608975705526 25.677500931072238 23.214091816407777 24.94231749546472 17 24.318665301443396 25.86242297363624 24.76398651960411 25.054925205316252 18 25.257485062348454 24.74850217570142 25.681192089539312 24.31282067241081 19 25.801891627042128 24.808146424015767 25.054908329389765 24.335053619552337 20 26.01995219645323 24.123674933175838 24.54931322147601 25.307059648894924 21 27.230656771842305 23.972759127731926 24.149466916844847 24.647117183580924 22 26.265717773680297 23.926499693703622 24.703352550006137 25.104429982609943 23 24.760728891474727 23.84217273794342 25.299143853572236 26.097954517009608 24 25.085825680423696 24.760890618388025 24.857295461902666 25.295988239285617 25 25.16375508922809 24.178552243996098 25.0560170270113 25.601675639764508 26 24.96733065303175 25.029235809595974 25.316739594098376 24.6866939432739 27 25.69118001261119 24.48306960783518 24.510639679794757 25.31511069975887 28 24.93429841122074 24.341301190831782 25.442659063132044 25.281741334815433 29 24.935196288522395 24.516285072059564 25.279959137213382 25.268559502204663 30 24.823090409857887 24.663965650160293 25.497780744077392 25.01516319590443 31 25.491061038251072 24.43566545604131 24.464386234594297 25.608887271113325 32 25.303606953099024 24.506978029617006 24.306586108482932 25.88282890880104 33 24.837542952594678 24.2336188157228 24.960810725784683 25.96802750589784 34 25.589310897376265 24.248200481224757 25.188696190760663 24.973792430638316 35 26.03415387748295 24.122651679904905 25.082360550285856 24.760833892326286 36 24.875653867999432 24.746495592279324 24.783673419680163 25.594177120041085 37 25.972486169679442 24.48516432998465 24.575855511121425 24.96649398921448 38 24.993682407254862 24.305428461338867 25.131570000449926 25.56931913095634 39 25.739212620586123 24.342040724095167 24.279832189178897 25.638914466139816 40 25.83173782736062 24.548815717368804 24.90900541908248 24.710441036188104 41 24.92984208040896 24.877984756043933 25.260064650827353 24.932108512719758 42 26.13147989726135 24.8778600106419 24.66766049700312 24.32299959509363 43 25.37679416126344 23.955570830131084 24.976592013682172 25.691042994923308 44 25.030029445939388 24.617718641596745 24.682327193265827 25.66992471919804 45 25.17671827806698 24.752229084122874 24.714884588934865 25.356168048875283 46 25.048803135036945 24.6535408699829 24.76408576362403 25.533570231356116 47 25.34461074461629 24.419717789593108 25.076972932453927 25.158698533336672 48 26.001068925391714 25.057742267204286 24.128656671988743 24.812532135415257 49 25.093149128922455 25.137055645251582 24.401367799610387 25.368427426215572 50 25.177682935368434 25.17105291198407 24.366519870281564 25.284744282365928 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 20.0 1 39.0 2 58.0 3 138.0 4 218.0 5 274.5 6 331.0 7 319.0 8 307.0 9 446.0 10 585.0 11 869.5 12 1154.0 13 2245.0 14 3336.0 15 4388.0 16 5440.0 17 5865.0 18 6290.0 19 6295.5 20 6301.0 21 7337.5 22 8374.0 23 9676.5 24 10979.0 25 13489.0 26 15999.0 27 20189.0 28 24379.0 29 28453.0 30 32527.0 31 36868.5 32 41210.0 33 47577.5 34 53945.0 35 63083.5 36 72222.0 37 87773.0 38 103324.0 39 114036.0 40 124748.0 41 137824.5 42 150901.0 43 154915.5 44 158930.0 45 175690.5 46 192451.0 47 205737.5 48 219024.0 49 227694.0 50 236364.0 51 229898.0 52 223432.0 53 220758.0 54 218084.0 55 219975.0 56 221866.0 57 215658.5 58 209451.0 59 191409.5 60 173368.0 61 151832.0 62 130296.0 63 113310.5 64 96325.0 65 81456.5 66 66588.0 67 57259.5 68 47931.0 69 43001.5 70 38072.0 71 29386.5 72 20701.0 73 18003.5 74 15306.0 75 12027.5 76 8749.0 77 7666.0 78 6583.0 79 5446.0 80 4309.0 81 3463.5 82 2618.0 83 2133.0 84 1648.0 85 1191.5 86 735.0 87 517.5 88 300.0 89 235.5 90 171.0 91 150.0 92 129.0 93 103.0 94 77.0 95 77.0 96 77.0 97 54.5 98 32.0 99 30.0 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008693319640509246 2 4.7356605045575657E-4 3 3.7208761107238015E-4 4 0.0 5 0.0 6 1.6913073230562735E-4 7 1.3530458584450186E-4 8 3.3826146461125465E-5 9 0.0012853935655227678 10 0.0012853935655227678 11 1.0147843938337642E-4 12 1.0147843938337642E-4 13 5.412183433780074E-4 14 2.3678302522787828E-4 15 0.004837138943940942 16 8.118275150670114E-4 17 0.0 18 1.6913073230562735E-4 19 6.765229292225093E-5 20 9.133059544503876E-4 21 0.0 22 2.0295687876675285E-4 23 7.780013686058858E-4 24 3.382614646112547E-4 25 0.001657481176595148 26 0.005344531140857825 27 0.006393141681152714 28 0.004194442161179558 29 0.0019619164947452775 30 0.004126789868257307 31 0.008287405882975739 32 0.0029090485956567904 33 0.004295920600562935 34 0.0029090485956567904 35 0.0028413963027345396 36 0.007780013686058859 37 0.003619397671340425 38 0.008862450372814874 39 0.003619397671340425 40 0.0024693086916621593 41 0.0035517453784181746 42 0.0018266119089007753 43 0.0012515674190616422 44 9.471321009115131E-4 45 0.001150088979678266 46 9.471321009115131E-4 47 0.0019957426412064026 48 0.0017927857624396501 49 4.059137575335057E-4 50 0.0014883504442895206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2956293.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.13830467700399 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0579274784047 38.63553845690732 2 13.005673525140576 13.041648434662854 3 4.135385741714035 6.220236908249892 4 1.8229855865278206 3.656056270364747 5 0.9813704475168272 2.4602125249303217 6 0.6177563110059711 1.858395248241564 7 0.426379434326334 1.496455939038364 8 0.30713519419331653 1.231939035479623 9 0.23447108191446595 1.0580384288676825 >10 1.2412817335304045 11.100893091637408 >50 0.08507207264129552 2.9594326300546414 >100 0.06668374208739675 7.079062383457527 >500 0.01162387194107229 3.9975393105290515 >1k 0.006253779055940039 5.204551337579062 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 3294 0.11142332644294729 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT 3271 0.11064532507434141 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 3269 0.11057767278141917 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 2958 0.10005774123200914 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.765229292225094E-5 2 0.0 0.0 0.0 0.0 6.765229292225094E-5 3 0.0 0.0 0.0 0.0 6.765229292225094E-5 4 0.0 0.0 0.0 0.0 6.765229292225094E-5 5 0.0 3.382614646112547E-5 0.0 0.0 1.353045858445019E-4 6 0.0 3.382614646112547E-5 0.0 0.0 1.6913073230562735E-4 7 0.0 3.382614646112547E-5 0.0 0.0 1.6913073230562735E-4 8 0.0 3.382614646112547E-5 0.0 0.0 1.6913073230562735E-4 9 0.0 3.382614646112547E-5 0.0 0.0 1.6913073230562735E-4 10 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 1.6913073230562735E-4 11 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 2.0295687876675282E-4 12 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 2.0295687876675282E-4 13 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 3.044353181501292E-4 14 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 3.044353181501292E-4 15 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 4.0591375753350564E-4 16 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 4.0591375753350564E-4 17 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 4.3973990399463113E-4 18 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 4.7356605045575657E-4 19 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 4.7356605045575657E-4 20 0.0 3.382614646112547E-5 0.0 3.382614646112547E-5 6.426967827613839E-4 21 0.0 6.765229292225094E-5 0.0 3.382614646112547E-5 6.426967827613839E-4 22 0.0 6.765229292225094E-5 0.0 6.765229292225094E-5 6.765229292225094E-4 23 0.0 6.765229292225094E-5 0.0 3.044353181501292E-4 6.765229292225094E-4 24 0.0 6.765229292225094E-5 0.0 6.765229292225094E-4 6.765229292225094E-4 25 0.0 6.765229292225094E-5 0.0 7.780013686058858E-4 6.765229292225094E-4 26 0.0 6.765229292225094E-5 0.0 9.809582473726387E-4 6.765229292225094E-4 27 0.0 6.765229292225094E-5 0.0 0.0013192197119838934 6.765229292225094E-4 28 0.0 6.765229292225094E-5 0.0 0.0034840930854959234 7.441752221447604E-4 29 0.0 6.765229292225094E-5 0.0 0.006629924706380592 7.441752221447604E-4 30 0.0 6.765229292225094E-5 0.0 0.012887761801688805 7.441752221447604E-4 31 0.0 1.0147843938337641E-4 0.0 0.02733152634058938 7.441752221447604E-4 32 0.0 1.0147843938337641E-4 0.0 0.044481382596379994 7.780013686058858E-4 33 0.0 1.0147843938337641E-4 0.0 0.0634916769075325 7.780013686058858E-4 34 0.0 1.0147843938337641E-4 0.0 0.08344910331959654 8.794798079892623E-4 35 0.0 1.0147843938337641E-4 0.0 0.10618027374147285 8.794798079892623E-4 36 0.0 1.0147843938337641E-4 0.0 0.14000642020259832 8.794798079892623E-4 37 0.0 1.0147843938337641E-4 0.0 0.19368851463640444 8.794798079892623E-4 38 0.0 1.0147843938337641E-4 0.0 0.26127315526573314 8.794798079892623E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4625 0.0 23.87812 1 GGTATCA 2225 0.0 21.158842 1 AACGCAG 6280 0.0 17.2348 6 TAGCGCA 145 1.2517376E-7 16.688816 9 ACCGTCG 865 0.0 16.53096 8 CGAGCCG 1040 0.0 16.499168 15 GGCGTTA 900 0.0 16.132246 42 TCAACGC 6725 0.0 16.028933 4 ATACCGT 990 0.0 15.776982 6 CAACGCA 6860 0.0 15.7455635 5 TATCAAC 6960 0.0 15.740054 2 TAAACGC 775 0.0 15.614493 28 TCTATAC 310 0.0 15.611853 3 ATCAACG 6905 0.0 15.610826 3 TACCGTC 965 0.0 15.501811 7 CGTCGTA 915 0.0 15.387465 10 CGCGGGA 715 0.0 15.38384 44 CCGTCGT 925 0.0 15.220855 9 GTATTAC 280 0.0 14.928073 1 GTAAACG 755 0.0 14.86244 27 >>END_MODULE