FastQCFastQC Report
Thu 2 Feb 2017
SRR4064003_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064003_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103288
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23320.21136820123122882No Hit
TATCAACGCAGAGTACTTTTTTTTT14320.12979385255708392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT602.5739258E-514.2458851
ATAGGAG852.7061105E-713.4078913
GTATAGG852.7061105E-713.4078911
ATAGGGG500.001502331513.296163
TAAATGT655.4505414E-513.15362554
GACGTGA957.3805495E-812.99978357
GGGTAGG2750.012.4327731
GTATCAA16450.012.41282751
TTAGACA853.941115E-612.2939124
AACCGTG550.00306785812.0907067
TTATACA550.00306785812.0907064
AACAGTC1103.8098733E-812.0907067
AGATCGT550.003068900412.0901586
TAAGGTT550.003068900412.0901585
CGGACAT1201.000808E-811.8742635
CTCGTAG1052.7158967E-711.76224110
AAGATCG907.460103E-611.6103915
GTCCTAA2050.011.582021
CTTACAC1406.82121E-1011.5323833
GGTATCA6300.011.4570081
Produced by FastQC (version 0.11.5)