##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064003_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1103288
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19274840295553	32.0	32.0	32.0	32.0	32.0
2	30.72419803351437	32.0	32.0	32.0	32.0	32.0
3	30.743791285684246	32.0	32.0	32.0	32.0	32.0
4	30.779574326920986	32.0	32.0	32.0	32.0	32.0
5	30.66317226327124	32.0	32.0	32.0	32.0	32.0
6	34.315427159544925	36.0	36.0	36.0	32.0	36.0
7	34.254892648157146	36.0	36.0	36.0	32.0	36.0
8	34.20917294487024	36.0	36.0	36.0	32.0	36.0
9	34.37253645467004	36.0	36.0	36.0	32.0	36.0
10	34.01695930708935	36.0	36.0	36.0	32.0	36.0
11	34.34920800371254	36.0	36.0	36.0	32.0	36.0
12	34.13065219598147	36.0	36.0	36.0	32.0	36.0
13	34.228357418915095	36.0	36.0	36.0	32.0	36.0
14	34.10663308220519	36.0	36.0	36.0	32.0	36.0
15	34.039835473602544	36.0	36.0	36.0	32.0	36.0
16	34.041887521662524	36.0	36.0	36.0	32.0	36.0
17	33.971178876231775	36.0	36.0	36.0	32.0	36.0
18	33.95624986404275	36.0	36.0	36.0	32.0	36.0
19	33.97596003944573	36.0	36.0	36.0	32.0	36.0
20	33.95537611213029	36.0	36.0	36.0	32.0	36.0
21	33.945677828454585	36.0	36.0	36.0	32.0	36.0
22	33.91902748874274	36.0	36.0	36.0	32.0	36.0
23	33.85196702946103	36.0	36.0	36.0	32.0	36.0
24	33.861648998267	36.0	36.0	36.0	32.0	36.0
25	33.42124087273677	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	5.0
5	11.0
6	40.0
7	16.0
8	64.0
9	71.0
10	97.0
11	29.0
12	80.0
13	59.0
14	92.0
15	155.0
16	293.0
17	426.0
18	538.0
19	817.0
20	1238.0
21	1881.0
22	3029.0
23	4791.0
24	6967.0
25	10024.0
26	14194.0
27	18327.0
28	25042.0
29	32743.0
30	42896.0
31	57155.0
32	79588.0
33	109928.0
34	228425.0
35	464264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25603106066514	17.180113050085506	11.483360746558537	26.080495142690825
2	16.802419069753107	19.530489451920445	37.68058272315755	25.986508755168895
3	18.927867681581613	23.548008752100557	28.525448708303802	28.998674858014027
4	12.676466136176792	15.47844246626126	35.66476351385035	36.18032788371159
5	14.873073210085252	36.42801136698407	33.36155439428206	15.337361028648608
6	34.235087649322935	35.34183145295364	16.802399682362985	13.620681215360433
7	30.458606140495615	30.23966536057688	20.610350686014446	18.691377812913064
8	28.11683401366219	32.91809396698516	19.299790189119935	19.665281830232715
9	27.700708944599306	14.199204545660638	18.355389992074475	39.74469651766558
10	16.237388508749255	26.362222433830762	31.269061773067975	26.131327284352
11	37.223246981494476	20.978817409662483	22.474240453868603	19.323695154974434
12	25.074708896049657	23.4800424083518	28.591083181497044	22.854165514101503
13	29.714431861158396	19.36135112265545	25.015758079246588	25.908458936939567
14	23.797737461759695	19.712271453798273	24.799218550890785	31.690772533551247
15	25.29352015598785	27.21344034825194	22.012605994649252	25.480433501110962
16	25.952257865571067	25.507759003800146	23.675799707960348	24.864183422668447
17	24.205247921292063	25.806270224776167	24.995193214632945	24.99328863929883
18	24.95285590278565	24.703053413649883	26.095017465312015	24.249073218252455
19	25.977694169209393	24.661403438755112	25.024308566411367	24.33659382562413
20	26.222470157106052	24.215013969014187	24.608595479119046	24.95392039476071
21	27.072467659218596	23.84083854734516	24.10995956381896	24.976734229617282
22	26.353828598795793	24.14051771211085	24.59014609067309	24.91550759842027
23	24.797649532528013	24.153974124176017	25.10887421207363	25.93950213122234
24	25.12068224243117	24.670878980337093	25.01138340580617	25.197055371425563
25	25.124970860541502	24.382810395326075	25.091772286810276	25.400446457322147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	82.0
2	200.0
3	318.0
4	318.0
5	318.0
6	840.0
7	1362.0
8	1362.0
9	1362.0
10	1530.0
11	1698.0
12	1698.0
13	1698.0
14	2175.5
15	2653.0
16	2653.0
17	2653.0
18	4482.0
19	6311.0
20	6311.0
21	6311.0
22	10529.5
23	14748.0
24	14748.0
25	14748.0
26	22249.5
27	29751.0
28	29751.0
29	29751.0
30	37671.5
31	45592.0
32	45592.0
33	45592.0
34	56804.0
35	68016.0
36	68016.0
37	68016.0
38	78104.5
39	88193.0
40	88193.0
41	88193.0
42	101794.5
43	115396.0
44	115396.0
45	115396.0
46	128617.0
47	141838.0
48	141838.0
49	141838.0
50	145988.0
51	150138.0
52	150138.0
53	150138.0
54	140934.5
55	131731.0
56	131731.0
57	131731.0
58	121079.5
59	110428.0
60	110428.0
61	110428.0
62	96816.0
63	83204.0
64	83204.0
65	83204.0
66	68759.0
67	54314.0
68	54314.0
69	54314.0
70	40806.0
71	27298.0
72	27298.0
73	27298.0
74	21010.5
75	14723.0
76	14723.0
77	14723.0
78	11659.5
79	8596.0
80	8596.0
81	8596.0
82	5959.5
83	3323.0
84	3323.0
85	3323.0
86	2569.5
87	1816.0
88	1816.0
89	1816.0
90	1310.0
91	804.0
92	804.0
93	804.0
94	571.0
95	338.0
96	338.0
97	338.0
98	477.5
99	617.0
100	617.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03752420039010666
2	0.004894460920448695
3	9.063816519349436E-4
4	0.0028097831209983252
5	0.008429349362994975
6	0.012870619457476197
7	0.022659541298373588
8	0.035348884425462794
9	0.047313122231004055
10	0.056467576915546984
11	0.05347651746416167
12	0.061180761505608694
13	0.061543314166382666
14	0.06535011710450943
15	0.0578271493934494
16	0.06326543930505907
17	0.06163395233157616
18	0.07405138096308489
19	0.0721479794940215
20	0.07686116408408321
21	0.07341691380673042
22	0.07450457178905236
23	0.07876456555314659
24	0.07305436114595644
25	0.0753203152757938
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1103288.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.8749672366585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91949433275703	44.65499127413114
2	11.87258210172665	13.267602718970295
3	3.3640272638273405	5.638947394487357
4	1.4917686083425126	3.3341008846325417
5	0.8437914752577352	2.3573410517298847
6	0.5384619986887559	1.8051927920951931
7	0.3642630279953864	1.4247229328337763
8	0.24803545855367395	1.108717849617288
9	0.19811838467773207	0.9962872427563185
>10	0.998624573510243	9.957154829749863
>50	0.07301578300125372	2.8468153108212544
>100	0.07983363013676421	9.14516546895378
>500	0.007005807052560628	2.7421474402483077
>1k	9.775544724503202E-4	0.7208128089730462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2332	0.21136820123122882	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1432	0.12979385255708392	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.063816519349435E-5	0.0
6	0.0	0.0	0.0	9.063816519349435E-5	9.063816519349435E-5
7	0.0	0.0	0.0	9.063816519349435E-5	9.063816519349435E-5
8	0.0	0.0	0.0	9.063816519349435E-5	9.063816519349435E-5
9	0.0	0.0	0.0	9.063816519349435E-5	1.812763303869887E-4
10	0.0	0.0	0.0	9.063816519349435E-5	1.812763303869887E-4
11	0.0	0.0	0.0	9.063816519349435E-5	2.7191449558048306E-4
12	0.0	0.0	0.0	9.063816519349435E-5	5.438289911609661E-4
13	0.0	0.0	0.0	9.063816519349435E-5	5.438289911609661E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAT	60	2.5739258E-5	14.245885	1
ATAGGAG	85	2.7061105E-7	13.407891	3
GTATAGG	85	2.7061105E-7	13.407891	1
ATAGGGG	50	0.0015023315	13.29616	3
TAAATGT	65	5.4505414E-5	13.1536255	4
GACGTGA	95	7.3805495E-8	12.9997835	7
GGGTAGG	275	0.0	12.432773	1
GTATCAA	1645	0.0	12.4128275	1
TTAGACA	85	3.941115E-6	12.293912	4
AACCGTG	55	0.003067858	12.090706	7
TTATACA	55	0.003067858	12.090706	4
AACAGTC	110	3.8098733E-8	12.090706	7
AGATCGT	55	0.0030689004	12.090158	6
TAAGGTT	55	0.0030689004	12.090158	5
CGGACAT	120	1.000808E-8	11.874263	5
CTCGTAG	105	2.7158967E-7	11.762241	10
AAGATCG	90	7.460103E-6	11.610391	5
GTCCTAA	205	0.0	11.58202	1
CTTACAC	140	6.82121E-10	11.532383	3
GGTATCA	630	0.0	11.457008	1
>>END_MODULE