FastQCFastQC Report
Thu 2 Feb 2017
SRR4064003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103288
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11440.10369006098135754No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11090.10051772519958524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATG450.001397263524.44706532
AGTCCGT551.5933173E-424.0003956
GGTATCA8700.023.0118731
TAGGACG3350.022.9854554
GTATCAA17850.022.9247471
TTAGGAC2700.022.8141563
ACCGTCG3100.021.291648
CGTCGTA3100.021.29067410
ATACCGT3250.020.984966
GTCCTAA2000.020.9003431
AGGACGT3800.020.842455
TGTAGGA5200.020.730172
CGGTCCA3800.020.26349310
TAGCGGC3700.020.21654930
CTAGCGG3700.020.21654929
GGACGTG4150.020.144916
TACCGTC3400.020.0600647
AATCACG1900.019.6863234
CGAATGC3800.019.6827543
GTCGTAG3600.019.55499311
Produced by FastQC (version 0.11.5)