##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064003_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1103288
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.328989348202825	32.0	32.0	32.0	32.0	32.0
2	31.390670432380304	32.0	32.0	32.0	32.0	32.0
3	31.473789255389345	32.0	32.0	32.0	32.0	32.0
4	31.55263811443612	32.0	32.0	32.0	32.0	32.0
5	31.504509248718378	32.0	32.0	32.0	32.0	32.0
6	35.08035979726055	36.0	36.0	36.0	36.0	36.0
7	35.099771773100045	36.0	36.0	36.0	36.0	36.0
8	35.04192468331025	36.0	36.0	36.0	36.0	36.0
9	35.166891147189126	36.0	36.0	36.0	36.0	36.0
10	35.00635826728833	36.0	36.0	36.0	36.0	36.0
11	35.15576168688502	36.0	36.0	36.0	36.0	36.0
12	35.05903082422722	36.0	36.0	36.0	36.0	36.0
13	35.10723220047712	36.0	36.0	36.0	36.0	36.0
14	35.04904159204124	36.0	36.0	36.0	36.0	36.0
15	35.01861435998579	36.0	36.0	36.0	36.0	36.0
16	35.02198338058603	36.0	36.0	36.0	36.0	36.0
17	34.99118181290832	36.0	36.0	36.0	36.0	36.0
18	34.99192413948126	36.0	36.0	36.0	36.0	36.0
19	34.99394537056507	36.0	36.0	36.0	36.0	36.0
20	34.982067239016466	36.0	36.0	36.0	36.0	36.0
21	34.96771377917643	36.0	36.0	36.0	36.0	36.0
22	34.96143164794687	36.0	36.0	36.0	36.0	36.0
23	34.90573177629051	36.0	36.0	36.0	32.0	36.0
24	34.87266153533801	36.0	36.0	36.0	32.0	36.0
25	34.85176490635265	36.0	36.0	36.0	32.0	36.0
26	34.78425125624497	36.0	36.0	36.0	32.0	36.0
27	34.77978551384589	36.0	36.0	36.0	32.0	36.0
28	34.75277443423657	36.0	36.0	36.0	32.0	36.0
29	34.72542527427109	36.0	36.0	36.0	32.0	36.0
30	34.71082437224007	36.0	36.0	36.0	32.0	36.0
31	34.70712452233687	36.0	36.0	36.0	32.0	36.0
32	34.658439138284834	36.0	36.0	36.0	32.0	36.0
33	34.647951396190294	36.0	36.0	36.0	32.0	36.0
34	34.63978580388802	36.0	36.0	36.0	32.0	36.0
35	34.59828530719087	36.0	36.0	36.0	32.0	36.0
36	34.572820514679755	36.0	36.0	36.0	32.0	36.0
37	34.55521767661753	36.0	36.0	36.0	32.0	36.0
38	34.520609306001695	36.0	36.0	36.0	32.0	36.0
39	34.520919288526656	36.0	36.0	36.0	32.0	36.0
40	34.50338442908833	36.0	36.0	36.0	32.0	36.0
41	34.48655110904859	36.0	36.0	36.0	32.0	36.0
42	34.41469045253823	36.0	36.0	36.0	32.0	36.0
43	34.4240216516449	36.0	36.0	36.0	32.0	36.0
44	34.3438966072322	36.0	36.0	36.0	32.0	36.0
45	34.32443478040185	36.0	36.0	36.0	32.0	36.0
46	34.29850863962991	36.0	36.0	36.0	32.0	36.0
47	34.267116111115136	36.0	36.0	36.0	32.0	36.0
48	34.22583133325116	36.0	36.0	36.0	32.0	36.0
49	34.20813604426043	36.0	36.0	36.0	32.0	36.0
50	33.595963157398614	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	17.0
21	36.0
22	118.0
23	325.0
24	869.0
25	2041.0
26	4138.0
27	7490.0
28	12775.0
29	19588.0
30	28409.0
31	40007.0
32	58264.0
33	93755.0
34	192513.0
35	642942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38007077695108	18.137409534583533	11.956663620937215	26.525856067528174
2	15.938779135743832	20.318068602463192	37.25495883199611	26.488193429796862
3	18.11392171009459	24.144010064498353	28.832558072082982	28.909510153324074
4	12.38226102341365	16.129242772512708	35.921083162329325	35.56741304174431
5	14.413371667234664	36.79746358158523	33.20175693019411	15.587407820985998
6	34.322710228616856	35.686181383447824	16.62622690197564	13.364881485959682
7	30.319372638875798	30.421249936553284	20.574500946262443	18.684876478308475
8	28.040120113805383	33.10634494922899	19.123945084098697	19.729589852866933
9	27.642422330748285	13.90015843838487	18.33335146129499	40.12406776957185
10	16.113073948723713	26.564474230429685	31.22105217361354	26.101399647233066
11	37.3216240908992	20.854391600379955	22.58431162126299	19.239672687457855
12	25.0504175250864	23.423914176456353	28.600627035395142	22.925041263062106
13	29.775125081574938	19.362174606627512	25.076589804945254	25.786110506852296
14	23.767864782359638	19.60793555263902	24.869390403956174	31.754809261045168
15	25.341767929559822	27.234022881818365	21.921822568920103	25.50238661970171
16	25.918288731277332	25.490380911377493	23.656749223901567	24.93458113344361
17	24.23292920796746	25.798069044528717	24.943532423084452	25.02546932441937
18	24.924090123830535	24.651539678885364	26.069944039692484	24.35442615759162
19	25.8596123586951	24.544180667241918	25.198769496269335	24.39743747779365
20	26.226610736673422	24.122981933935414	24.705334256637457	24.945073072753708
21	27.168971293080318	23.86910761288077	24.12398213340488	24.83793896063403
22	26.24165222498568	24.20963519951273	24.685213652282993	24.863498923218597
23	24.709501520924952	23.928101399831046	25.227594908255053	26.134802170988948
24	25.114045988200672	24.54229785105	25.081597378007274	25.262058782742052
25	25.1771323636713	24.20357283024751	25.251275525233602	25.368019280847587
26	24.84688018085225	25.096966065729347	25.475947249009945	24.580206504408455
27	25.403997737540156	24.60687947325294	24.73423348295541	25.254889306251492
28	24.76942809102322	24.551795401704986	25.412174196793973	25.266602310477822
29	24.91862774333953	24.509568434724994	25.19208928424138	25.379714537694092
30	24.841943252755737	24.637908420900924	25.499278037567425	25.02087028877591
31	25.485603440852415	24.456520261236477	24.538553228518467	25.51932306939264
32	25.109313194084397	24.616134844985552	24.45080543701711	25.823746523912945
33	24.823406031523596	24.286168792380966	25.054272110163094	25.836153065932344
34	25.50466482424313	24.406915161199976	25.166031124207688	24.92238889034921
35	25.844342423102546	24.324566849912305	25.108882352674584	24.722208374310565
36	24.733662374626654	24.876972094941557	24.893106902162337	25.49625862826945
37	25.817296492788067	24.488665667791533	24.61456744265306	25.079470396767345
38	25.022751911704454	24.637601023926674	25.022751911704454	25.316895152664422
39	25.72543474418415	24.30008112505495	24.49623153725181	25.478252593509087
40	25.963689429508914	24.498667585156717	24.995921179051177	24.5417218062832
41	24.933311217039698	24.78538584321542	25.372555311128213	24.90874762861667
42	26.033147368468757	25.02939030414546	24.7258340093632	24.21162831802258
43	25.17604405066733	24.323582062495753	25.015431329450955	25.484942557385963
44	24.965285205152654	24.788901417233767	24.668532624656024	25.577280752957556
45	25.076408804324572	24.961659639111154	24.732795782741583	25.229135773822687
46	24.995875926103846	24.625343748357167	24.793388429752067	25.58539189578692
47	25.382298476969638	24.475985283627356	25.036414774019928	25.10530146538308
48	25.843658028335998	25.03723053747028	24.208868073828405	24.910243360365317
49	25.19931404787888	24.987673164842416	24.418735339223627	25.39427744805508
50	25.1162896091654	25.41005003262998	24.27316728301066	25.200493075193968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	16.5
2	22.0
3	50.0
4	78.0
5	92.5
6	107.0
7	107.0
8	107.0
9	161.0
10	215.0
11	308.0
12	401.0
13	833.5
14	1266.0
15	1712.5
16	2159.0
17	2306.0
18	2453.0
19	2494.5
20	2536.0
21	2779.5
22	3023.0
23	3543.5
24	4064.0
25	5050.0
26	6036.0
27	7619.0
28	9202.0
29	10916.0
30	12630.0
31	14514.5
32	16399.0
33	19403.0
34	22407.0
35	26423.5
36	30440.0
37	35064.0
38	39688.0
39	43152.0
40	46616.0
41	50954.5
42	55293.0
43	56514.5
44	57736.0
45	63792.5
46	69849.0
47	75169.0
48	80489.0
49	83631.0
50	86773.0
51	84949.0
52	83125.0
53	82514.0
54	81903.0
55	82111.0
56	82319.0
57	80280.5
58	78242.0
59	71355.5
60	64469.0
61	56521.0
62	48573.0
63	42195.0
64	35817.0
65	30217.0
66	24617.0
67	21027.0
68	17437.0
69	15786.0
70	14135.0
71	10900.0
72	7665.0
73	6646.5
74	5628.0
75	4374.0
76	3120.0
77	2743.0
78	2366.0
79	1995.0
80	1624.0
81	1313.0
82	1002.0
83	817.0
84	632.0
85	463.5
86	295.0
87	213.0
88	131.0
89	101.0
90	71.0
91	54.0
92	37.0
93	34.5
94	32.0
95	29.0
96	26.0
97	19.5
98	13.0
99	11.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009426369180123413
2	3.6255266077397745E-4
3	3.6255266077397745E-4
4	0.0
5	0.0
6	9.063816519349436E-5
7	0.0
8	9.063816519349436E-5
9	0.0018127633038698872
10	9.063816519349436E-4
11	0.0
12	9.063816519349436E-5
13	7.251053215479549E-4
14	0.0
15	0.004894460920448695
16	0.0011782961475154266
17	0.0
18	5.438289911609661E-4
19	0.0
20	3.6255266077397745E-4
21	0.0
22	0.0
23	0.0010876579823219323
24	4.531908259674718E-4
25	0.001540848808289404
26	0.006072757067964122
27	0.005800842572383639
28	0.0048038227552552005
29	0.002265954129837359
30	0.004078717433707246
31	0.0066165860591250875
32	0.0032629739469657968
33	0.004622546424868212
34	0.0028097831209983252
35	0.002991059451385314
36	0.007522967711060031
37	0.004259993764094235
38	0.007613605876253526
39	0.0048038227552552005
40	0.002537868625417842
41	0.0026285067906113365
42	0.002991059451385314
43	0.0011782961475154266
44	0.0010876579823219323
45	0.0010876579823219323
46	9.063816519349436E-4
47	0.0024472304602243477
48	0.0024472304602243477
49	3.6255266077397745E-4
50	7.251053215479549E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1103288.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.03764349856597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68357547873326	47.63368165303093
2	11.677714026851548	13.7884943519093
3	3.280020163991102	5.809339833294437
4	1.3725006197135827	3.241168091528454
5	0.7915911907689206	2.336683925861043
6	0.47020464488194985	1.665586451754622
7	0.3139566452398878	1.297468234697477
8	0.22787565763766623	1.0762593470091049
9	0.17001471454451592	0.903354129611063
>10	0.8754309208471756	9.173265894270566
>50	0.06517873181226876	2.654960136539702
>100	0.06529922429332247	7.641597173083313
>500	0.006174865753405047	2.4779342452187594
>1k	4.6311493150537857E-4	0.3002065321913492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1144	0.10369006098135754	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1109	0.10051772519958524	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.063816519349435E-5	0.0
6	0.0	0.0	0.0	9.063816519349435E-5	0.0
7	0.0	0.0	0.0	9.063816519349435E-5	0.0
8	0.0	0.0	0.0	9.063816519349435E-5	0.0
9	0.0	0.0	0.0	9.063816519349435E-5	9.063816519349435E-5
10	0.0	0.0	0.0	9.063816519349435E-5	9.063816519349435E-5
11	0.0	0.0	0.0	9.063816519349435E-5	9.063816519349435E-5
12	0.0	0.0	0.0	9.063816519349435E-5	2.7191449558048306E-4
13	0.0	0.0	0.0	9.063816519349435E-5	2.7191449558048306E-4
14	0.0	0.0	0.0	9.063816519349435E-5	2.7191449558048306E-4
15	0.0	0.0	0.0	9.063816519349435E-5	3.625526607739774E-4
16	0.0	0.0	0.0	9.063816519349435E-5	5.438289911609661E-4
17	0.0	0.0	0.0	9.063816519349435E-5	6.344671563544605E-4
18	0.0	0.0	0.0	9.063816519349435E-5	7.251053215479548E-4
19	0.0	0.0	0.0	9.063816519349435E-5	7.251053215479548E-4
20	0.0	0.0	0.0	9.063816519349435E-5	7.251053215479548E-4
21	0.0	0.0	0.0	9.063816519349435E-5	9.063816519349436E-4
22	0.0	0.0	0.0	9.063816519349435E-5	9.063816519349436E-4
23	0.0	0.0	0.0	6.344671563544605E-4	9.063816519349436E-4
24	0.0	0.0	0.0	8.157434867414492E-4	9.063816519349436E-4
25	0.0	0.0	0.0	8.157434867414492E-4	9.063816519349436E-4
26	0.0	0.0	0.0	8.157434867414492E-4	9.970198171284379E-4
27	0.0	0.0	0.0	0.0010876579823219323	9.970198171284379E-4
28	0.0	0.0	0.0	0.0021753159646438645	9.970198171284379E-4
29	0.0	0.0	0.0	0.004894460920448695	9.970198171284379E-4
30	0.0	0.0	0.0	0.007885520371834008	9.970198171284379E-4
31	9.063816519349435E-5	0.0	0.0	0.019305929186214297	9.970198171284379E-4
32	9.063816519349435E-5	0.0	0.0	0.032720377634851465	9.970198171284379E-4
33	9.063816519349435E-5	0.0	0.0	0.045681635257521155	9.970198171284379E-4
34	9.063816519349435E-5	0.0	0.0	0.06254033398351111	9.970198171284379E-4
35	9.063816519349435E-5	0.0	0.0	0.07958030903988804	9.970198171284379E-4
36	9.063816519349435E-5	0.0	0.0	0.10622792960677538	9.970198171284379E-4
37	9.063816519349435E-5	0.0	0.0	0.14547425513555845	9.970198171284379E-4
38	9.063816519349435E-5	0.0	0.0	0.1944188643400454	9.970198171284379E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTATG	45	0.0013972635	24.447065	32
AGTCCGT	55	1.5933173E-4	24.000395	6
GGTATCA	870	0.0	23.011873	1
TAGGACG	335	0.0	22.985455	4
GTATCAA	1785	0.0	22.924747	1
TTAGGAC	270	0.0	22.814156	3
ACCGTCG	310	0.0	21.29164	8
CGTCGTA	310	0.0	21.290674	10
ATACCGT	325	0.0	20.98496	6
GTCCTAA	200	0.0	20.900343	1
AGGACGT	380	0.0	20.84245	5
TGTAGGA	520	0.0	20.73017	2
CGGTCCA	380	0.0	20.263493	10
TAGCGGC	370	0.0	20.216549	30
CTAGCGG	370	0.0	20.216549	29
GGACGTG	415	0.0	20.14491	6
TACCGTC	340	0.0	20.060064	7
AATCACG	190	0.0	19.68632	34
CGAATGC	380	0.0	19.68275	43
GTCGTAG	360	0.0	19.554993	11
>>END_MODULE