FastQCFastQC Report
Thu 2 Feb 2017
SRR4064002_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064002_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006915
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27230.27042997671104313No Hit
TATCAACGCAGAGTACTTTTTTTTT20420.20279765422106136No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13240.1314907415223728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAC400.005273820414.25145313
AACTTAT400.005277300314.2500377
CGCGGTC1001.006083E-813.29937510
GTATTAA655.4638935E-513.1499591
GTACTAA655.4638935E-513.1499591
TTTAGGA2100.013.1119122
CGAACGA801.991777E-613.06578116
ATATACG1457.2759576E-1212.4470716
ACATCTA1001.4394573E-712.3506478
AACGAAC853.930545E-612.29659514
TACGCTA1551.8189894E-1212.2587069
AGGGACG2100.012.2149258
CGACCCG701.0921615E-412.2118895
AACCGCG1103.8073267E-812.090947
ACGCTAT1501.4551915E-1112.03276710
ATACGCT1603.6379788E-1211.8756218
GTATTAG1603.6379788E-1211.87149051
ACAATAC1201.00444595E-811.87093
CTTAATC2100.011.7578453
CGTCGTA1701.8189894E-1211.73474110
Produced by FastQC (version 0.11.5)