##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064002_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1006915
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.283653535799942	32.0	32.0	32.0	32.0	32.0
2	30.896242483228477	32.0	32.0	32.0	32.0	32.0
3	30.914767383542802	32.0	32.0	32.0	32.0	32.0
4	30.951040554565182	32.0	32.0	32.0	32.0	32.0
5	30.86539280872765	32.0	32.0	32.0	32.0	32.0
6	34.533837513593504	36.0	36.0	36.0	32.0	36.0
7	34.468090156567335	36.0	36.0	36.0	32.0	36.0
8	34.41330300968801	36.0	36.0	36.0	32.0	36.0
9	34.550452620131786	36.0	36.0	36.0	32.0	36.0
10	34.27376888813852	36.0	36.0	36.0	32.0	36.0
11	34.54508672529459	36.0	36.0	36.0	32.0	36.0
12	34.35915345386651	36.0	36.0	36.0	32.0	36.0
13	34.44539509293237	36.0	36.0	36.0	32.0	36.0
14	34.340030687793906	36.0	36.0	36.0	32.0	36.0
15	34.28456225202723	36.0	36.0	36.0	32.0	36.0
16	34.282505474642846	36.0	36.0	36.0	32.0	36.0
17	34.207260791625906	36.0	36.0	36.0	32.0	36.0
18	34.20590615891113	36.0	36.0	36.0	32.0	36.0
19	34.2136416678667	36.0	36.0	36.0	32.0	36.0
20	34.182638057830104	36.0	36.0	36.0	32.0	36.0
21	34.16719981329109	36.0	36.0	36.0	32.0	36.0
22	34.16400689233947	36.0	36.0	36.0	32.0	36.0
23	34.11388051623027	36.0	36.0	36.0	32.0	36.0
24	34.09187170714509	36.0	36.0	36.0	32.0	36.0
25	33.70177323805882	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	12.0
5	13.0
6	42.0
7	15.0
8	66.0
9	70.0
10	86.0
11	25.0
12	52.0
13	44.0
14	115.0
15	154.0
16	266.0
17	368.0
18	435.0
19	586.0
20	923.0
21	1297.0
22	2086.0
23	3222.0
24	4937.0
25	7242.0
26	10469.0
27	13992.0
28	19275.0
29	25883.0
30	34648.0
31	47474.0
32	67239.0
33	95964.0
34	207669.0
35	462238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.268024091070764	17.41893306998214	11.606296584051488	25.706746254895613
2	16.314825022719486	20.014600062571695	38.05570867412561	25.614866240583208
3	18.46088603433738	24.381785118746716	28.831621297637977	28.325707549277922
4	12.31693194526382	15.800612991202403	36.55968122868386	35.32277383484992
5	14.274717721750324	36.938125243343315	33.82670777347816	14.960449261428197
6	33.43325655336407	36.11121871563898	17.282048684261497	13.17347604673546
7	29.94727201204757	30.740476578362852	21.09387727531003	18.21837413427955
8	27.876432379374133	33.05763693914412	19.609415205724154	19.456515475757595
9	27.52499232419424	14.068832850262666	18.669546874083995	39.7366279514591
10	15.79601643983719	26.837755978122612	31.96159135716103	25.40463622487917
11	36.783332687452116	21.033370198245787	23.096811632221325	19.08648548208077
12	24.8093708168658	23.779133495582215	29.26025044893384	22.15124523861815
13	29.59898875165088	19.702349542523407	25.36051063271591	25.3381510731098
14	23.57302787906253	20.00538639428605	25.5462171511937	30.875368575457717
15	24.944079257901198	27.585744309797434	22.573397526668554	24.896778905632814
16	25.387333811682723	25.761002864133538	24.4029742466494	24.44868907753434
17	23.68170001669489	25.89884089770803	25.660541232400803	24.758917853196277
18	24.411593113942367	25.076305795662474	26.710854952894653	23.80124613750051
19	25.47595668386697	24.919000898444022	25.70066707480938	23.904375342879632
20	25.736452445531988	24.394890974525442	25.265345513203417	24.603311066739156
21	26.67845400948553	24.25787446181736	24.733244282244247	24.330427246452864
22	25.80752885238472	24.584756240582973	25.064502920024328	24.54321198700798
23	24.381400313884363	24.529695269768602	25.585750166732428	25.503154249614603
24	24.6087041218752	25.135368297182115	25.64603078684562	24.609896794097068
25	24.73798299833919	24.69932027149384	25.67145428505263	24.891242445114333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	187.0
1	187.0
2	307.0
3	427.0
4	427.0
5	427.0
6	1185.5
7	1944.0
8	1944.0
9	1944.0
10	1942.5
11	1941.0
12	1941.0
13	1941.0
14	2354.5
15	2768.0
16	2768.0
17	2768.0
18	4795.5
19	6823.0
20	6823.0
21	6823.0
22	11136.5
23	15450.0
24	15450.0
25	15450.0
26	23470.5
27	31491.0
28	31491.0
29	31491.0
30	39113.0
31	46735.0
32	46735.0
33	46735.0
34	56521.5
35	66308.0
36	66308.0
37	66308.0
38	75713.5
39	85119.0
40	85119.0
41	85119.0
42	96409.0
43	107699.0
44	107699.0
45	107699.0
46	119716.0
47	131733.0
48	131733.0
49	131733.0
50	134545.0
51	137357.0
52	137357.0
53	137357.0
54	126543.0
55	115729.0
56	115729.0
57	115729.0
58	105203.5
59	94678.0
60	94678.0
61	94678.0
62	82306.0
63	69934.0
64	69934.0
65	69934.0
66	56969.5
67	44005.0
68	44005.0
69	44005.0
70	33212.0
71	22419.0
72	22419.0
73	22419.0
74	17215.5
75	12012.0
76	12012.0
77	12012.0
78	9416.5
79	6821.0
80	6821.0
81	6821.0
82	4710.0
83	2599.0
84	2599.0
85	2599.0
86	1979.5
87	1360.0
88	1360.0
89	1360.0
90	995.0
91	630.0
92	630.0
93	630.0
94	433.0
95	236.0
96	236.0
97	236.0
98	373.0
99	510.0
100	510.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03982461280247092
2	0.006951927421877716
3	0.0023835179732152167
4	0.004866349195314401
5	0.010626517630584508
6	0.014102481341523367
7	0.0237358664832682
8	0.03704384183371983
9	0.05025250393528749
10	0.057502371103817104
11	0.05343052789957445
12	0.06375910578350705
13	0.06296459979243531
14	0.06713575624556195
15	0.05760168435270108
16	0.0672350694944459
17	0.0614749010591758
18	0.076073948645119
19	0.07259798493418014
20	0.07686845463619074
21	0.0745842499118595
22	0.07537875590293124
23	0.08064235809378151
24	0.07657051488953884
25	0.07726570763172662
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1006915.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.117202169551284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89424753190664	41.07184337190161
2	14.26565675578593	15.440348614685846
3	4.632762670937299	7.521364621999926
4	1.8912601505333562	4.093988316865185
5	0.916243294440229	2.479226180085879
6	0.5353710987259972	1.7383671591293757
7	0.36292697345496516	1.3748414676672005
8	0.2502708751769616	1.083516763928173
9	0.18430315890396276	0.8976574179803419
>10	0.8907261345957566	8.697413577311172
>50	0.086922673564975	3.334254524198879
>100	0.08118971113054778	8.772641018586668
>500	0.0075654046494834665	2.886500515198207
>1k	5.535661938646439E-4	0.608036450461561
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2723	0.27042997671104313	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2042	0.20279765422106136	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1324	0.1314907415223728	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.931324888396737E-5
2	0.0	0.0	0.0	0.0	9.931324888396737E-5
3	0.0	0.0	0.0	0.0	9.931324888396737E-5
4	0.0	0.0	0.0	0.0	9.931324888396737E-5
5	0.0	0.0	0.0	0.0	9.931324888396737E-5
6	0.0	0.0	0.0	0.0	9.931324888396737E-5
7	0.0	0.0	0.0	0.0	9.931324888396737E-5
8	0.0	0.0	0.0	0.0	9.931324888396737E-5
9	0.0	0.0	0.0	0.0	9.931324888396737E-5
10	0.0	0.0	0.0	0.0	9.931324888396737E-5
11	0.0	0.0	0.0	0.0	9.931324888396737E-5
12	0.0	0.0	0.0	0.0	5.958794933038042E-4
13	0.0	0.0	0.0	0.0	8.938192399557063E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTAC	40	0.0052738204	14.251453	13
AACTTAT	40	0.0052773003	14.250037	7
CGCGGTC	100	1.006083E-8	13.299375	10
GTATTAA	65	5.4638935E-5	13.149959	1
GTACTAA	65	5.4638935E-5	13.149959	1
TTTAGGA	210	0.0	13.111912	2
CGAACGA	80	1.991777E-6	13.065781	16
ATATACG	145	7.2759576E-12	12.447071	6
ACATCTA	100	1.4394573E-7	12.350647	8
AACGAAC	85	3.930545E-6	12.296595	14
TACGCTA	155	1.8189894E-12	12.258706	9
AGGGACG	210	0.0	12.214925	8
CGACCCG	70	1.0921615E-4	12.211889	5
AACCGCG	110	3.8073267E-8	12.09094	7
ACGCTAT	150	1.4551915E-11	12.032767	10
ATACGCT	160	3.6379788E-12	11.875621	8
GTATTAG	160	3.6379788E-12	11.8714905	1
ACAATAC	120	1.00444595E-8	11.8709	3
CTTAATC	210	0.0	11.757845	3
CGTCGTA	170	1.8189894E-12	11.734741	10
>>END_MODULE