##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064002_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1006915
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.337731586082242	32.0	32.0	32.0	32.0	32.0
2	31.44276925063188	32.0	32.0	32.0	32.0	32.0
3	31.513177378428168	32.0	32.0	32.0	32.0	32.0
4	31.590863181102677	32.0	32.0	32.0	32.0	32.0
5	31.53964237299077	32.0	32.0	32.0	32.0	32.0
6	35.136727529136024	36.0	36.0	36.0	36.0	36.0
7	35.16307533406494	36.0	36.0	36.0	36.0	36.0
8	35.11415462079719	36.0	36.0	36.0	36.0	36.0
9	35.22059558155356	36.0	36.0	36.0	36.0	36.0
10	35.076711539702956	36.0	36.0	36.0	36.0	36.0
11	35.212391314063254	36.0	36.0	36.0	36.0	36.0
12	35.127334482056575	36.0	36.0	36.0	36.0	36.0
13	35.16926552886788	36.0	36.0	36.0	36.0	36.0
14	35.11039164179697	36.0	36.0	36.0	36.0	36.0
15	35.08460297045927	36.0	36.0	36.0	36.0	36.0
16	35.099403623940454	36.0	36.0	36.0	36.0	36.0
17	35.06491511200051	36.0	36.0	36.0	36.0	36.0
18	35.05651321114493	36.0	36.0	36.0	36.0	36.0
19	35.0559024346643	36.0	36.0	36.0	36.0	36.0
20	35.04987511358953	36.0	36.0	36.0	36.0	36.0
21	35.049210707954494	36.0	36.0	36.0	36.0	36.0
22	35.02792489932119	36.0	36.0	36.0	36.0	36.0
23	34.975582844629386	36.0	36.0	36.0	36.0	36.0
24	34.94716237219626	36.0	36.0	36.0	36.0	36.0
25	34.927116986041526	36.0	36.0	36.0	36.0	36.0
26	34.864762169597235	36.0	36.0	36.0	32.0	36.0
27	34.86354756856338	36.0	36.0	36.0	32.0	36.0
28	34.84254182329194	36.0	36.0	36.0	32.0	36.0
29	34.81531112358044	36.0	36.0	36.0	32.0	36.0
30	34.796220137747476	36.0	36.0	36.0	32.0	36.0
31	34.78271651529672	36.0	36.0	36.0	32.0	36.0
32	34.760052238768914	36.0	36.0	36.0	32.0	36.0
33	34.71514676015354	36.0	36.0	36.0	32.0	36.0
34	34.71832875664778	36.0	36.0	36.0	32.0	36.0
35	34.67268637372569	36.0	36.0	36.0	32.0	36.0
36	34.6486764026755	36.0	36.0	36.0	32.0	36.0
37	34.63015150236117	36.0	36.0	36.0	32.0	36.0
38	34.59432424782628	36.0	36.0	36.0	32.0	36.0
39	34.589544301157495	36.0	36.0	36.0	32.0	36.0
40	34.569228782965794	36.0	36.0	36.0	32.0	36.0
41	34.54347884379516	36.0	36.0	36.0	32.0	36.0
42	34.488709573300625	36.0	36.0	36.0	32.0	36.0
43	34.47663010283887	36.0	36.0	36.0	32.0	36.0
44	34.414499734337056	36.0	36.0	36.0	32.0	36.0
45	34.38039258527284	36.0	36.0	36.0	32.0	36.0
46	34.34583753345615	36.0	36.0	36.0	32.0	36.0
47	34.307054716634475	36.0	36.0	36.0	32.0	36.0
48	34.26122065914203	36.0	36.0	36.0	32.0	36.0
49	34.24880153736909	36.0	36.0	36.0	32.0	36.0
50	33.653437479827	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	1.0
20	6.0
21	33.0
22	98.0
23	266.0
24	768.0
25	1604.0
26	3431.0
27	6429.0
28	10850.0
29	16950.0
30	24084.0
31	34393.0
32	49523.0
33	80951.0
34	170885.0
35	606640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.40311712871366	18.339153615726282	12.121582322233081	26.136146933326977
2	15.693709387007615	20.756907498463125	37.46172708069118	26.087656033838076
3	17.668808533036717	24.81115491950621	29.316820768489738	28.203215778967333
4	12.011242259773665	16.45441770159348	36.58829196108907	34.946048077543786
5	13.936330276140488	37.480422875813744	33.61872650620956	14.964520341836202
6	33.53027170145613	36.42009148745573	17.0055238083888	13.044113002699337
7	29.763088244787294	30.993976651455185	21.10019217113659	18.142742932620926
8	27.99305601074173	33.36978133187144	19.46491954625718	19.17224311112965
9	27.539142222950524	13.908660697881132	18.496183850512214	40.05601322865613
10	15.642671709176428	27.16102595475645	31.960778861184657	25.23552347488246
11	36.8664056711894	21.09892205292607	22.999779524368517	19.03489275151602
12	24.731208424949898	23.914952021721817	29.19395300889649	22.1598865444318
13	29.629103635670244	19.702416894425728	25.402915051166335	25.265564418737686
14	23.534628646793365	20.03740148096358	25.548806648445943	30.879163223797114
15	25.00009931749021	27.64740701896567	22.554207486155143	24.798286177388984
16	25.36381214948352	25.835057259508577	24.22855338179196	24.572577209215947
17	23.800122155296126	26.00805430448449	25.57177120213722	24.620052338082164
18	24.450151651481654	25.147529977733946	26.69989691274528	23.702421458039115
19	25.509899048082506	24.917793458236297	25.665324282585917	23.906983211095277
20	25.84252813061743	24.42770456148017	25.226087733759723	24.503679574142677
21	26.7593590322917	24.279507207659037	24.648654553760743	24.312479206288515
22	25.79713858383139	24.5999161795347	25.16769058727955	24.435254649354363
23	24.463576856115942	24.41828938495443	25.667667427085682	25.450466331843945
24	24.87476512253354	24.944483507927238	25.60939033158938	24.571361037949842
25	24.951459685468695	24.533243287532464	25.683909444380994	24.83138758261785
26	24.41771655302899	25.48738398279792	26.109916522240045	23.98498294193305
27	25.02279386204499	25.019317673933557	25.299697075036004	24.65819138898545
28	24.335990068280573	25.03260086902545	25.92774674115456	24.703662321539415
29	24.539076683497335	24.893335134254716	25.630355589278693	24.937232592969256
30	24.396428177709183	25.20456737191386	25.883892932729886	24.51511151764707
31	25.086954362616083	24.76396682723345	25.115955703431492	25.033123106718975
32	24.66503192513162	25.12397121027484	24.956425100334002	25.25457176425954
33	24.37643885356518	24.79992610822761	25.65464733562557	25.168987702581642
34	25.180010448054695	24.86736333606784	25.643915994462134	24.308710221415332
35	25.522177717702743	24.705603100242428	25.565082149075025	24.2071370329798
36	24.422360297798296	25.352483373987937	25.311166641505835	24.91398968670793
37	25.43538088564939	25.1406080385191	24.96114222491923	24.462868850912276
38	24.66680637673847	25.145826525778	25.44835511254441	24.739011984939122
39	25.203130105565087	24.891569591614385	24.949372657957806	24.955927644862726
40	25.572556232669374	24.981328608544345	25.323571241289994	24.122543917496287
41	24.574971644912868	25.424432451329558	25.715730071488586	24.284865832268984
42	25.628644269626545	25.625962742665777	25.11855825220059	23.626834735507092
43	24.820191041432036	24.944532834377128	25.417170687912034	24.818105436278803
44	24.520782082846278	25.422558920886964	24.987064269476345	25.069594726790417
45	24.658905597940624	25.44795641301174	25.087843353798668	24.80529463524897
46	24.60187485040823	25.248905807586997	24.98790851588081	25.161310826123962
47	24.966530670618102	25.029596024201204	25.446125304402063	24.557748000778634
48	25.37551495504944	25.561931662435793	24.53827849086886	24.52427489164591
49	24.754720129187188	25.678237699260407	24.797424995853657	24.769617175698748
50	24.888866377461028	25.80882236099855	24.63809569210723	24.66421556943319
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	77.0
1	50.0
2	23.0
3	77.0
4	131.0
5	144.0
6	157.0
7	143.5
8	130.0
9	213.0
10	296.0
11	421.5
12	547.0
13	1015.5
14	1484.0
15	2053.0
16	2622.0
17	2916.5
18	3211.0
19	3169.0
20	3127.0
21	3491.5
22	3856.0
23	4484.0
24	5112.0
25	6012.5
26	6913.0
27	8583.5
28	10254.0
29	11996.5
30	13739.0
31	15616.0
32	17493.0
33	20405.0
34	23317.0
35	27125.5
36	30934.0
37	35605.5
38	40277.0
39	42775.0
40	45273.0
41	49178.0
42	53083.0
43	54515.0
44	55947.0
45	60686.5
46	65426.0
47	69306.0
48	73186.0
49	75576.5
50	77967.0
51	75744.0
52	73521.0
53	72506.5
54	71492.0
55	71670.0
56	71848.0
57	68961.5
58	66075.0
59	59981.0
60	53887.0
61	47496.0
62	41105.0
63	35370.0
64	29635.0
65	25240.5
66	20846.0
67	17830.0
68	14814.0
69	13118.5
70	11423.0
71	8830.5
72	6238.0
73	5341.0
74	4444.0
75	3385.0
76	2326.0
77	2040.0
78	1754.0
79	1466.0
80	1178.0
81	980.5
82	783.0
83	653.0
84	523.0
85	359.0
86	195.0
87	144.0
88	93.0
89	71.0
90	49.0
91	36.0
92	23.0
93	23.0
94	23.0
95	26.5
96	30.0
97	22.0
98	14.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00903750564844103
2	1.9862649776793473E-4
3	4.965662444198368E-4
4	0.0
5	0.0
6	9.931324888396737E-5
7	0.0
8	9.931324888396737E-5
9	9.931324888396737E-4
10	8.938192399557063E-4
11	9.931324888396737E-5
12	9.931324888396737E-5
13	2.979397466519021E-4
14	2.979397466519021E-4
15	0.004270469702010597
16	7.945059910717389E-4
17	0.0
18	9.931324888396737E-5
19	0.0
20	4.965662444198368E-4
21	0.0
22	9.931324888396737E-5
23	0.0013903854843755432
24	6.951927421877716E-4
25	0.0019862649776793474
26	0.005958794933038042
27	0.00645536117745788
28	0.003972529955358695
29	0.0022842047243312497
30	0.0033766504620548906
31	0.00645536117745788
32	0.0027807709687510864
33	0.003674590208706793
34	0.002880084217635054
35	0.0025821444709831515
36	0.005859481684154074
37	0.00377390345759076
38	0.006753300924109781
39	0.004767035946430433
40	0.002681457719867119
41	0.004071843204242662
42	0.0025821444709831515
43	0.0012910722354915758
44	0.0013903854843755432
45	8.938192399557063E-4
46	7.945059910717389E-4
47	0.0022842047243312497
48	0.0030787107154029887
49	3.9725299553586946E-4
50	0.00188695172879538
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1006915.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.294323808010006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.58096063733294	43.87654356283821
2	14.165460243606473	16.232009321733628
3	4.371881127660215	7.514519189348768
4	1.8587067764163374	4.259733916485603
5	0.8864709648608703	2.539487725356888
6	0.5277586572065959	1.8142545239085155
7	0.33611755284692696	1.3480339537257424
8	0.19575475865342848	0.8972509223398704
9	0.1644698764796748	0.8480871323720905
>10	0.7685949214671018	7.9517981246506375
>50	0.0706128054844909	2.8347736004026056
>100	0.06728501866925007	7.5997408007134855
>500	0.0059266593157740605	2.2837672261240582
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9862649776793473E-4
2	0.0	0.0	0.0	0.0	1.9862649776793473E-4
3	0.0	0.0	0.0	0.0	1.9862649776793473E-4
4	0.0	0.0	0.0	0.0	1.9862649776793473E-4
5	0.0	0.0	0.0	0.0	1.9862649776793473E-4
6	0.0	0.0	0.0	0.0	1.9862649776793473E-4
7	0.0	0.0	0.0	0.0	1.9862649776793473E-4
8	0.0	0.0	0.0	0.0	1.9862649776793473E-4
9	0.0	0.0	0.0	0.0	1.9862649776793473E-4
10	0.0	0.0	0.0	0.0	1.9862649776793473E-4
11	0.0	0.0	0.0	0.0	1.9862649776793473E-4
12	0.0	0.0	0.0	0.0	2.979397466519021E-4
13	0.0	0.0	0.0	0.0	4.965662444198368E-4
14	0.0	0.0	0.0	0.0	5.958794933038042E-4
15	0.0	0.0	0.0	0.0	8.938192399557063E-4
16	0.0	0.0	0.0	9.931324888396737E-5	8.938192399557063E-4
17	0.0	0.0	0.0	9.931324888396737E-5	8.938192399557063E-4
18	0.0	0.0	0.0	9.931324888396737E-5	8.938192399557063E-4
19	0.0	0.0	0.0	1.9862649776793473E-4	8.938192399557063E-4
20	0.0	0.0	0.0	1.9862649776793473E-4	0.0010924457377236411
21	0.0	0.0	0.0	5.958794933038042E-4	0.0010924457377236411
22	0.0	0.0	0.0	9.931324888396737E-4	0.0010924457377236411
23	0.0	0.0	0.0	0.0016883252310274453	0.0010924457377236411
24	0.0	0.0	0.0	0.002482831222099184	0.0010924457377236411
25	0.0	0.0	0.0	0.003277337213170923	0.0010924457377236411
26	0.0	0.0	0.0	0.003475963710938858	0.0010924457377236411
27	0.0	0.0	0.0	0.004071843204242662	0.0010924457377236411
28	0.0	0.0	0.0	0.005064975693082336	0.0010924457377236411
29	0.0	0.0	0.0	0.00715055391964565	0.0011917589866076083
30	0.0	0.0	0.0	0.013010035603799725	0.0011917589866076083
31	0.0	0.0	0.0	0.02562281821206358	0.0011917589866076083
32	0.0	0.0	0.0	0.039923926051354884	0.0011917589866076083
33	0.0	0.0	0.0	0.052536708659618736	0.0011917589866076083
34	0.0	0.0	0.0	0.06614262375672227	0.0011917589866076083
35	0.0	0.0	0.0	0.08540939404021193	0.0011917589866076083
36	0.0	0.0	0.0	0.11152877849669535	0.0011917589866076083
37	0.0	0.0	0.0	0.15194927079247006	0.0011917589866076083
38	0.0	0.0	0.0	0.20369147346101707	0.0013903854843755432
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	40	1.8197014E-5	33.000187	28
AACCGTA	30	0.0057457997	29.33204	7
GTATAAT	230	0.0	22.003403	1
TATGACG	50	0.0025808013	21.999031	4
CCGTCGT	290	0.0	21.241499	9
ATACCGT	305	0.0	20.917112	6
CGTCTTA	100	1.122995E-6	19.800112	15
CGTCGTA	325	0.0	19.63088	10
ACCGTCG	315	0.0	19.554695	8
TACCGTC	315	0.0	19.554695	7
CGCAATA	275	0.0	19.201061	36
GTATCAA	1865	0.0	18.640953	1
TAGGACG	320	0.0	18.561684	4
TTAACGG	60	0.0074089007	18.334347	35
GTTCGAC	60	0.007411023	18.333437	11
GGACGTG	360	0.0	18.332527	6
TACGAAT	295	0.0	17.897518	41
CGAATGC	295	0.0	17.897518	43
GTCCTAA	225	0.0	17.60272	1
CGTGCAA	100	2.4822259E-5	17.6001	11
>>END_MODULE