FastQCFastQC Report
Thu 2 Feb 2017
SRR4064001_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064001_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970863
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46520.4791613234823039No Hit
TATCAACGCAGAGTACTTTTTTTTT34540.3557659525597329No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29120.2999393323259822No Hit
GTACATGGGGTGGTATCAACGCAAA23120.23813864572035395No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19260.1983802040040665No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18230.187771086136767No Hit
GGTATCAACGCAGAGTACTTTTTTT16510.1700548893098202No Hit
GTATCAACGCAGAGTACATGGGGTG12520.1289574327170775No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9830.1012501248888875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT954.9039954E-913.9976841
GCACGCG551.9591379E-413.81803119
GCTCGTA1051.366061E-913.572689
GTTATTC1001.0091753E-813.296433
CGTTATT957.406379E-812.9958392
GCATCGC1251.0186341E-1012.92052614
TAAGACC701.09019646E-412.2141524
TTAAGAC701.0928225E-412.2110053
CTCGTAG1103.8018698E-812.09202510
AATCCCG1103.8073267E-812.09077719
CGAGGTT550.003067266712.0907774
AGGCCCG1900.012.00110610
ATCGCCA1353.6925485E-1011.964066516
CGCATCG1353.6925485E-1011.9634513
CCGTCGT1209.977157E-911.8760969
GTATTAG1201.0015356E-811.8730361
ATTTAGA1701.8189894E-1211.7333531
TTAGAGT907.4532672E-611.6109854
AAAGCTC1251.8266292E-811.4004646
AAATCCT1850.011.2971755
Produced by FastQC (version 0.11.5)