##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064001_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	970863
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16012145894941	32.0	32.0	32.0	32.0	32.0
2	30.79735657863159	32.0	32.0	32.0	32.0	32.0
3	30.823365397589566	32.0	32.0	32.0	32.0	32.0
4	30.83846536535021	32.0	32.0	32.0	32.0	32.0
5	30.777769881023378	32.0	32.0	32.0	32.0	32.0
6	34.408388207192985	36.0	36.0	36.0	32.0	36.0
7	34.33174196565324	36.0	36.0	36.0	32.0	36.0
8	34.269888748464	36.0	36.0	36.0	32.0	36.0
9	34.38442396095021	36.0	36.0	36.0	32.0	36.0
10	34.10964265812993	36.0	36.0	36.0	32.0	36.0
11	34.397573087036996	36.0	36.0	36.0	32.0	36.0
12	34.218792970789906	36.0	36.0	36.0	32.0	36.0
13	34.30501007866197	36.0	36.0	36.0	32.0	36.0
14	34.20654510471611	36.0	36.0	36.0	32.0	36.0
15	34.15546992727089	36.0	36.0	36.0	32.0	36.0
16	34.14763051017497	36.0	36.0	36.0	32.0	36.0
17	34.06576520065138	36.0	36.0	36.0	32.0	36.0
18	34.059720063489905	36.0	36.0	36.0	32.0	36.0
19	34.06507509298428	36.0	36.0	36.0	32.0	36.0
20	34.01141767685039	36.0	36.0	36.0	32.0	36.0
21	33.99734154046452	36.0	36.0	36.0	32.0	36.0
22	33.99466145068872	36.0	36.0	36.0	32.0	36.0
23	33.95064803169963	36.0	36.0	36.0	32.0	36.0
24	33.93469418445239	36.0	36.0	36.0	32.0	36.0
25	33.536211597310846	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	6.0
5	12.0
6	32.0
7	22.0
8	64.0
9	70.0
10	81.0
11	36.0
12	62.0
13	43.0
14	176.0
15	246.0
16	331.0
17	537.0
18	638.0
19	844.0
20	1255.0
21	1814.0
22	2814.0
23	4106.0
24	6001.0
25	8343.0
26	11683.0
27	15191.0
28	20381.0
29	27052.0
30	34913.0
31	47034.0
32	65906.0
33	91229.0
34	193968.0
35	435970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.27529780305841	17.008367338526853	11.548472857672808	25.16786200074193
2	16.159289665331013	19.695408988370534	38.620121341972165	25.525180004326288
3	18.791175513264747	24.985991570221376	28.341525518002893	27.881307398510984
4	12.248229857873605	15.426348284862582	35.633161864226956	36.692259993036856
5	14.243848271088112	37.3174671193592	33.24519151531133	15.193493094241356
6	33.66861400040588	35.5106317005055	17.37879469965356	13.441959599435064
7	30.043889020533054	30.490815243707697	20.85830852126969	18.60698721448956
8	28.53764666242835	32.80457408519842	19.11539384921964	19.5423854031536
9	28.076776420879064	13.72339877363838	18.392950997062915	39.80687380841964
10	16.377368135730173	26.856426714817815	31.194564856669942	25.571640292782067
11	37.02720055734708	20.874338227640415	22.835152853054332	19.263308361958174
12	25.567758998493122	23.539451958219697	29.033784159848615	21.85900488343857
13	30.303370751773606	19.695959282580173	25.270382674069957	24.730287291576264
14	23.783510223265775	19.78346487127766	25.619235015033155	30.81378989042341
15	24.837002788937422	27.79898625319759	22.98203990262424	24.38197105524075
16	25.024324680893166	25.586162967116326	25.020923348395396	24.368589003595105
17	23.35067968209698	26.029726880038094	25.824930712519574	24.794662725345354
18	24.28287359793503	24.732936324267822	27.5579406795473	23.426249398249848
19	25.650741554687855	24.494497610614726	25.923799431825213	23.93096140287221
20	26.0011339621669	24.032987990309778	25.65960517499098	24.306272872532343
21	26.984539297027123	23.741698337451307	24.88545432243075	24.38830804309082
22	26.08363992095554	24.34946556339792	25.316492232140003	24.25040228350653
23	24.293250065203566	24.531694086526418	26.132763734241614	25.042292114028402
24	24.819169059019387	24.89916040016287	26.04254178671381	24.239128754103938
25	24.737187165621048	24.775019019010763	25.93986900076488	24.5479248146033
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	168.0
1	168.0
2	472.0
3	776.0
4	776.0
5	776.0
6	2164.0
7	3552.0
8	3552.0
9	3552.0
10	3324.0
11	3096.0
12	3096.0
13	3096.0
14	2724.5
15	2353.0
16	2353.0
17	2353.0
18	4218.0
19	6083.0
20	6083.0
21	6083.0
22	10287.5
23	14492.0
24	14492.0
25	14492.0
26	22377.5
27	30263.0
28	30263.0
29	30263.0
30	38333.5
31	46404.0
32	46404.0
33	46404.0
34	54207.0
35	62010.0
36	62010.0
37	62010.0
38	69675.5
39	77341.0
40	77341.0
41	77341.0
42	89142.0
43	100943.0
44	100943.0
45	100943.0
46	114809.5
47	128676.0
48	128676.0
49	128676.0
50	129639.0
51	130602.0
52	130602.0
53	130602.0
54	119394.0
55	108186.0
56	108186.0
57	108186.0
58	99886.5
59	91587.0
60	91587.0
61	91587.0
62	81184.5
63	70782.0
64	70782.0
65	70782.0
66	58134.5
67	45487.0
68	45487.0
69	45487.0
70	34409.0
71	23331.0
72	23331.0
73	23331.0
74	17720.5
75	12110.0
76	12110.0
77	12110.0
78	9601.0
79	7092.0
80	7092.0
81	7092.0
82	4864.5
83	2637.0
84	2637.0
85	2637.0
86	2016.0
87	1395.0
88	1395.0
89	1395.0
90	1025.5
91	656.0
92	656.0
93	656.0
94	451.0
95	246.0
96	246.0
97	246.0
98	420.5
99	595.0
100	595.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04356948405696787
2	0.005459060650163824
3	0.001957021742511559
4	0.003811042340680405
5	0.008137090403074377
6	0.01380215334192363
7	0.02399926663185228
8	0.03625640280863521
9	0.052839587047812095
10	0.060255669440487485
11	0.05685663167717793
12	0.0664357381010503
13	0.06839275984356186
14	0.069422771286989
15	0.061903687749970904
16	0.06746574954447744
17	0.0648907209359096
18	0.0799288880099458
19	0.0761178456692654
20	0.0837399303506262
21	0.07477883079281011
22	0.0799288880099458
23	0.08487294293839605
24	0.07807486741177695
25	0.08044389373165936
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	970863.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.35421634730782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.73098137115743	34.68495392025653
2	15.403937429335521	14.896906461169648
3	5.687373680155056	8.250254921346055
4	2.6157630856417717	5.059326966256948
5	1.3436731504060102	3.2486131107400453
6	0.7747050930711111	2.247615460543305
7	0.5202252667851957	1.7608559569627096
8	0.34650848489474234	1.3404116995822573
9	0.2503171106711491	1.0893498952343166
>10	1.126717044262005	9.635409285615374
>50	0.09423924084569782	3.248354266897057
>100	0.0946612469258935	9.330812203995272
>500	0.009188337676196801	3.1425079328616525
>1k	0.0017094581723156841	2.064627918538881
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4652	0.4791613234823039	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3454	0.3557659525597329	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2912	0.2999393323259822	No Hit
GTACATGGGGTGGTATCAACGCAAA	2312	0.23813864572035395	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1926	0.1983802040040665	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1823	0.187771086136767	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1651	0.1700548893098202	No Hit
GTATCAACGCAGAGTACATGGGGTG	1252	0.1289574327170775	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	983	0.1012501248888875	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0600228868542729E-4
2	0.0	0.0	0.0	0.0	2.0600228868542729E-4
3	0.0	0.0	0.0	0.0	2.0600228868542729E-4
4	0.0	0.0	0.0	0.0	2.0600228868542729E-4
5	0.0	0.0	0.0	0.0	2.0600228868542729E-4
6	0.0	0.0	0.0	1.0300114434271364E-4	2.0600228868542729E-4
7	0.0	0.0	0.0	1.0300114434271364E-4	2.0600228868542729E-4
8	0.0	0.0	0.0	1.0300114434271364E-4	2.0600228868542729E-4
9	0.0	0.0	0.0	1.0300114434271364E-4	2.0600228868542729E-4
10	0.0	0.0	0.0	1.0300114434271364E-4	3.0900343302814093E-4
11	0.0	0.0	0.0	1.0300114434271364E-4	3.0900343302814093E-4
12	0.0	0.0	0.0	1.0300114434271364E-4	0.002060022886854273
13	0.0	1.0300114434271364E-4	0.0	1.0300114434271364E-4	0.0023690263198824137
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	95	4.9039954E-9	13.997684	1
GCACGCG	55	1.9591379E-4	13.818031	19
GCTCGTA	105	1.366061E-9	13.57268	9
GTTATTC	100	1.0091753E-8	13.29643	3
CGTTATT	95	7.406379E-8	12.995839	2
GCATCGC	125	1.0186341E-10	12.920526	14
TAAGACC	70	1.09019646E-4	12.214152	4
TTAAGAC	70	1.0928225E-4	12.211005	3
CTCGTAG	110	3.8018698E-8	12.092025	10
AATCCCG	110	3.8073267E-8	12.090777	19
CGAGGTT	55	0.0030672667	12.090777	4
AGGCCCG	190	0.0	12.001106	10
ATCGCCA	135	3.6925485E-10	11.9640665	16
CGCATCG	135	3.6925485E-10	11.96345	13
CCGTCGT	120	9.977157E-9	11.876096	9
GTATTAG	120	1.0015356E-8	11.873036	1
ATTTAGA	170	1.8189894E-12	11.733353	1
TTAGAGT	90	7.4532672E-6	11.610985	4
AAAGCTC	125	1.8266292E-8	11.400464	6
AAATCCT	185	0.0	11.297175	5
>>END_MODULE