FastQCFastQC Report
Thu 2 Feb 2017
SRR4064001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970863
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14440.14873365243087852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12380.12751541669627947No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10020.10320714663139907No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA9890.10186813175494379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAC500.00258102321.998614
TGTAATA1158.772986E-921.0421472
GATATAC2200.020.9997541
CTTACGG550.004484627419.9987372
GTAATAC1001.1236516E-619.7987483
TAGTACA1001.1236516E-619.7987484
TAGGACG5850.019.5543214
ACTGTTC3300.019.3321118
ACGTATG708.1167655E-418.85886422
TAATACT1404.081812E-918.8559514
GACGTGA3150.018.8559517
CTTAGTA708.1252033E-418.8559512
TGTAGGA8250.018.6654872
GTCCTAC5950.018.4871791
CTAAACG600.007411577318.33311812
ACGGAAT2400.018.33311818
GTAGGAC7800.017.7681083
CGTATGT750.001289831917.60160823
CGCGGGA2250.017.59979444
TAGGACC6650.017.5327264
Produced by FastQC (version 0.11.5)