##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064001_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	970863
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229702851998685	32.0	32.0	32.0	32.0	32.0
2	31.39314712786459	32.0	32.0	32.0	32.0	32.0
3	31.47077703033281	32.0	32.0	32.0	32.0	32.0
4	31.5631330064077	32.0	32.0	32.0	32.0	32.0
5	31.49911367515293	32.0	32.0	32.0	32.0	32.0
6	35.07137670299517	36.0	36.0	36.0	36.0	36.0
7	35.10091228113544	36.0	36.0	36.0	36.0	36.0
8	35.05269744546862	36.0	36.0	36.0	36.0	36.0
9	35.16802061670905	36.0	36.0	36.0	36.0	36.0
10	35.004828693646786	36.0	36.0	36.0	36.0	36.0
11	35.15934586033251	36.0	36.0	36.0	36.0	36.0
12	35.06614733489689	36.0	36.0	36.0	36.0	36.0
13	35.10498906642853	36.0	36.0	36.0	36.0	36.0
14	35.048277666364875	36.0	36.0	36.0	36.0	36.0
15	35.01724651160875	36.0	36.0	36.0	36.0	36.0
16	35.031455519470825	36.0	36.0	36.0	36.0	36.0
17	34.99467793087181	36.0	36.0	36.0	36.0	36.0
18	34.991959730672605	36.0	36.0	36.0	36.0	36.0
19	34.9799652474139	36.0	36.0	36.0	36.0	36.0
20	34.971206030099	36.0	36.0	36.0	36.0	36.0
21	34.96150332230191	36.0	36.0	36.0	36.0	36.0
22	34.94772073917741	36.0	36.0	36.0	36.0	36.0
23	34.89541263803441	36.0	36.0	36.0	32.0	36.0
24	34.873787547779656	36.0	36.0	36.0	32.0	36.0
25	34.83558030329717	36.0	36.0	36.0	32.0	36.0
26	34.7795610709235	36.0	36.0	36.0	32.0	36.0
27	34.77185246528089	36.0	36.0	36.0	32.0	36.0
28	34.748939860721855	36.0	36.0	36.0	32.0	36.0
29	34.71167713673299	36.0	36.0	36.0	32.0	36.0
30	34.68507194114927	36.0	36.0	36.0	32.0	36.0
31	34.67412085948275	36.0	36.0	36.0	32.0	36.0
32	34.62236175443909	36.0	36.0	36.0	32.0	36.0
33	34.591085456959426	36.0	36.0	36.0	32.0	36.0
34	34.56786178894448	36.0	36.0	36.0	32.0	36.0
35	34.50651842741973	36.0	36.0	36.0	32.0	36.0
36	34.4817631323884	36.0	36.0	36.0	32.0	36.0
37	34.457961627953686	36.0	36.0	36.0	32.0	36.0
38	34.400999935109276	36.0	36.0	36.0	32.0	36.0
39	34.3820827449393	36.0	36.0	36.0	32.0	36.0
40	34.34655044017539	36.0	36.0	36.0	32.0	36.0
41	34.32661456868786	36.0	36.0	36.0	32.0	36.0
42	34.23483230898695	36.0	36.0	36.0	32.0	36.0
43	34.22082415335635	36.0	36.0	36.0	32.0	36.0
44	34.12921390556649	36.0	36.0	36.0	32.0	36.0
45	34.07135713277775	36.0	36.0	36.0	32.0	36.0
46	34.02339258989167	36.0	36.0	36.0	32.0	36.0
47	33.9493522773038	36.0	36.0	36.0	32.0	36.0
48	33.88664312060507	36.0	36.0	36.0	32.0	36.0
49	33.835302200207444	36.0	36.0	36.0	32.0	36.0
50	33.25407807280739	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	2.0
20	6.0
21	24.0
22	124.0
23	390.0
24	952.0
25	2209.0
26	4370.0
27	7865.0
28	12972.0
29	19220.0
30	27050.0
31	37495.0
32	52549.0
33	83724.0
34	168516.0
35	553389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.404613273668986	17.93855149140265	12.060603813079183	25.59623142184918
2	15.56547122430355	20.45044878014614	38.00798060264468	25.976099392905628
3	18.174261635536254	25.299476135640287	28.819955874264313	27.706306354559146
4	11.899310201336338	16.118855080479943	35.718427831733216	36.26340688645051
5	13.767853960857504	37.6969768134124	33.02577191632599	15.509397309404108
6	33.6484138338777	35.86953051048397	17.07130666221702	13.410748993421318
7	29.673805675981058	30.800535193946004	20.853714684770146	18.67194444530279
8	28.4942365709683	33.11085086155307	18.946236492687436	19.44867607479119
9	27.937053357147416	13.459490304412402	18.400501002210426	40.20295533622975
10	16.224495366445797	27.06640449172017	31.16784930365359	25.541250838180446
11	37.235776042321156	20.82005475546473	22.727431911023398	19.216737291190718
12	25.398949182325413	23.599828194091234	29.079489073123604	21.921733550459745
13	30.39768864717879	19.635065817934617	25.36132913087366	24.605916404012937
14	23.74523618235379	19.814803370207855	25.575160167274376	30.86480028016398
15	24.89400625455804	27.84009674375677	22.844072796792787	24.4218242048924
16	24.852861501317395	25.536177427295968	25.027347057333028	24.583614014053605
17	23.43451135742118	26.06031953014998	25.79200154913721	24.713167563291627
18	24.35230171981365	24.585290788279682	27.66358932947147	23.3988181624352
19	25.704683054852286	24.317462213991277	26.14614641421747	23.83170831693897
20	26.12589135533392	23.802985621922165	25.95130261738904	24.11982040535488
21	26.89009674897488	23.558524735209808	25.168638623575106	24.382739892240203
22	26.0103907661439	24.275643706314597	25.52391586033854	24.19004966720296
23	24.186029264835845	24.29273899993614	26.50325794297415	25.017973792253862
24	24.975640103908088	24.622138355969668	26.207540134602585	24.194681405519653
25	24.754055977693795	24.492429837328284	26.289101005200592	24.46441317977733
26	24.03629145648929	25.38187301904498	26.800692619645304	23.781142904820424
27	24.749639988380743	25.006953014105864	25.92938621881702	24.314020778696378
28	23.881387235366283	24.944711857877287	26.54613662840703	24.6277642783494
29	24.161344814799556	24.71179597049977	26.212764204194304	24.914095010506365
30	23.902841497858525	25.031571053927276	26.539565277402954	24.52602217081125
31	24.792308112267445	24.79024793235193	25.55189644711673	24.865547508263898
32	24.34576599404633	25.19370023588064	25.4239156185944	25.036618151478628
33	23.91123416808814	24.901732960866234	26.199393504898932	24.987639366146695
34	24.70460994916747	24.869828549650556	26.287474184593673	24.138087316588297
35	24.92393169612232	24.937013178305754	26.267822231927468	23.87123289364446
36	23.9799460641009	25.551256615800455	25.96999120296833	24.498806117130314
37	24.891020860543783	25.49679242873094	25.368139361452286	24.244047349272993
38	24.227634571645183	25.622486567557768	25.70746942677259	24.44240943402446
39	24.94550998125296	24.887826785603924	25.492058260027605	24.67460497311551
40	25.330490405117274	25.320292945211833	25.738285796689432	23.61093085298147
41	24.353182328523015	25.45574405405684	26.172862396094065	24.018211221326084
42	25.32881217663412	26.113800068600174	25.32901818316659	23.228369571599117
43	24.3721931523217	25.28130278933707	25.75820938568662	24.58829467265461
44	24.00911776460264	25.841013872351297	25.30385681854701	24.846011544499056
45	24.180460421280323	26.097543389813048	25.141473966112173	24.58052222279446
46	24.121212745466625	25.491860267496175	25.36454980403871	25.02237718299849
47	24.99878970663519	25.173792977002368	25.263097176771844	24.5643201395906
48	25.087475884260936	25.924998892710327	24.469453740527523	24.51807148250122
49	24.606662759473828	26.033852495573505	24.654455487357072	24.705029257595594
50	24.374313748514194	26.64314098721332	24.348872230016045	24.633673034256436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	36.0
1	32.5
2	29.0
3	94.0
4	159.0
5	178.0
6	197.0
7	231.5
8	266.0
9	349.5
10	433.0
11	659.0
12	885.0
13	1699.0
14	2513.0
15	3566.0
16	4619.0
17	5252.5
18	5886.0
19	5938.5
20	5991.0
21	6144.0
22	6297.0
23	6679.5
24	7062.0
25	6787.0
26	6512.0
27	7744.0
28	8976.0
29	10618.5
30	12261.0
31	14619.0
32	16977.0
33	20925.5
34	24874.0
35	28885.0
36	32896.0
37	35967.5
38	39039.0
39	40238.0
40	41437.0
41	44277.5
42	47118.0
43	47739.0
44	48360.0
45	53587.0
46	58814.0
47	62861.0
48	66908.0
49	69351.5
50	71795.0
51	70003.0
52	68211.0
53	67547.0
54	66883.0
55	67277.5
56	67672.0
57	65948.0
58	64224.0
59	59125.0
60	54026.0
61	47930.5
62	41835.0
63	36170.5
64	30506.0
65	25789.5
66	21073.0
67	18171.5
68	15270.0
69	13511.5
70	11753.0
71	9155.0
72	6557.0
73	5579.5
74	4602.0
75	3671.5
76	2741.0
77	2367.5
78	1994.0
79	1666.0
80	1338.0
81	1081.0
82	824.0
83	678.0
84	532.0
85	374.0
86	216.0
87	162.5
88	109.0
89	81.5
90	54.0
91	42.5
92	31.0
93	26.5
94	22.0
95	23.5
96	25.0
97	22.0
98	19.0
99	12.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010197113289928651
2	9.270102990844228E-4
3	1.0300114434271364E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	9.270102990844228E-4
10	0.0010300114434271365
11	1.0300114434271364E-4
12	0.0
13	3.09003433028141E-4
14	3.09003433028141E-4
15	0.005253058361478396
16	9.270102990844228E-4
17	0.0
18	2.0600228868542729E-4
19	1.0300114434271364E-4
20	0.0010300114434271365
21	0.0
22	1.0300114434271364E-4
23	5.150057217135683E-4
24	5.150057217135683E-4
25	0.001236013732112564
26	0.005768064083191964
27	0.006283069804905532
28	0.004120045773708546
29	0.002369026319882414
30	0.003811042340680405
31	0.00762208468136081
32	0.0033990377633095503
33	0.004017044629365832
34	0.002884032041595982
35	0.002575028608567841
36	0.008343092691759806
37	0.0036050400519949777
38	0.008137090403074377
39	0.004429049206736687
40	0.0033990377633095503
41	0.0033990377633095503
42	0.002060022886854273
43	0.002369026319882414
44	0.0011330125877698502
45	0.0013390148764552774
46	8.240091547417091E-4
47	0.002472027464225128
48	0.002472027464225128
49	4.1200457737085457E-4
50	9.270102990844228E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	970863.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.34898997610995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.69199428281938	38.053524800547535
2	15.101332421615016	15.81078999130057
3	5.453904208194042	8.565191301762413
4	2.5100128329808595	5.255865465344893
5	1.3229771193567048	3.4628257979913473
6	0.7646516515903042	2.401724498659005
7	0.47075730896359885	1.725056875368115
8	0.3141016035356616	1.3154321355974727
9	0.2205503702224931	1.0391030208004168
>10	0.988788514095062	8.920599380768394
>50	0.07858923444993245	2.8853726749987
>100	0.07481896875548462	7.725219337230607
>500	0.006927682098728084	2.4575721538645774
>1k	5.938013227481216E-4	0.3817225657659627
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1444	0.14873365243087852	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1238	0.12751541669627947	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1002	0.10320714663139907	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	989	0.10186813175494379	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0600228868542729E-4
2	0.0	0.0	0.0	0.0	2.0600228868542729E-4
3	0.0	0.0	0.0	0.0	2.0600228868542729E-4
4	0.0	0.0	0.0	0.0	2.0600228868542729E-4
5	0.0	0.0	0.0	0.0	2.0600228868542729E-4
6	0.0	0.0	0.0	1.0300114434271364E-4	2.0600228868542729E-4
7	0.0	0.0	0.0	1.0300114434271364E-4	2.0600228868542729E-4
8	0.0	0.0	0.0	1.0300114434271364E-4	2.0600228868542729E-4
9	0.0	0.0	0.0	1.0300114434271364E-4	2.0600228868542729E-4
10	0.0	0.0	0.0	2.0600228868542729E-4	3.0900343302814093E-4
11	0.0	0.0	0.0	2.0600228868542729E-4	3.0900343302814093E-4
12	0.0	0.0	0.0	2.0600228868542729E-4	0.001442016020797991
13	0.0	0.0	0.0	2.0600228868542729E-4	0.0018540205981688457
14	0.0	0.0	0.0	2.0600228868542729E-4	0.0018540205981688457
15	0.0	0.0	0.0	3.0900343302814093E-4	0.002060022886854273
16	0.0	0.0	0.0	4.1200457737085457E-4	0.0021630240311969868
17	0.0	0.0	0.0	4.1200457737085457E-4	0.0021630240311969868
18	0.0	0.0	0.0	4.1200457737085457E-4	0.002575028608567841
19	0.0	0.0	0.0	5.150057217135683E-4	0.002575028608567841
20	0.0	0.0	0.0	6.180068660562819E-4	0.0027810308972532685
21	0.0	0.0	0.0	9.270102990844228E-4	0.002987033185938696
22	0.0	0.0	0.0	0.0013390148764552774	0.002987033185938696
23	0.0	0.0	0.0	0.002987033185938696	0.002987033185938696
24	0.0	0.0	0.0	0.004944054928450255	0.002987033185938696
25	0.0	0.0	0.0	0.0062830698049055325	0.002987033185938696
26	0.0	0.0	0.0	0.00762208468136081	0.0030900343302814096
27	0.0	0.0	0.0	0.009167101846501515	0.003193035474624123
28	0.0	0.0	0.0	0.011948132743754784	0.003193035474624123
29	0.0	0.0	0.0	0.018025200259974887	0.003193035474624123
30	1.0300114434271364E-4	0.0	0.0	0.026986299817790976	0.003193035474624123
31	1.0300114434271364E-4	0.0	0.0	0.05242758247044125	0.0036050400519949777
32	1.0300114434271364E-4	0.0	0.0	0.07931088114388951	0.0036050400519949777
33	2.0600228868542729E-4	0.0	0.0	0.09888109856900511	0.0036050400519949777
34	2.0600228868542729E-4	0.0	0.0	0.12236535947914381	0.004017044629365832
35	2.0600228868542729E-4	0.0	0.0	0.15192668790550262	0.004017044629365832
36	2.0600228868542729E-4	0.0	0.0	0.19292114335390267	0.004017044629365832
37	2.0600228868542729E-4	0.0	0.0	0.2482327578659399	0.004017044629365832
38	2.0600228868542729E-4	0.0	0.0	0.323732596669149	0.004017044629365832
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAC	50	0.002581023	21.99861	4
TGTAATA	115	8.772986E-9	21.042147	2
GATATAC	220	0.0	20.999754	1
CTTACGG	55	0.0044846274	19.998737	2
GTAATAC	100	1.1236516E-6	19.798748	3
TAGTACA	100	1.1236516E-6	19.798748	4
TAGGACG	585	0.0	19.554321	4
ACTGTTC	330	0.0	19.332111	8
ACGTATG	70	8.1167655E-4	18.858864	22
TAATACT	140	4.081812E-9	18.855951	4
GACGTGA	315	0.0	18.855951	7
CTTAGTA	70	8.1252033E-4	18.855951	2
TGTAGGA	825	0.0	18.665487	2
GTCCTAC	595	0.0	18.487179	1
CTAAACG	60	0.0074115773	18.333118	12
ACGGAAT	240	0.0	18.333118	18
GTAGGAC	780	0.0	17.768108	3
CGTATGT	75	0.0012898319	17.601608	23
CGCGGGA	225	0.0	17.599794	44
TAGGACC	665	0.0	17.532726	4
>>END_MODULE