FastQCFastQC Report
Thu 2 Feb 2017
SRR4064000_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064000_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278585
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7490.2688586966276002No Hit
TATCAACGCAGAGTACTTTTTTTTT4520.16224850584202308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG3370.12096846563885348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAC307.6956104E-419.0019761
CCCTTAA307.6956104E-419.0019761
TTATATA250.006022556718.9985644
GGTATCA607.6091965E-817.4184781
TGTTACA400.00526736114.2489222
GTATCAA2650.013.6240581
GTACAAG604.069742E-412.6679831
TGCACTG604.075915E-412.66570955
AAGGTTG550.003059317812.0899955
TGCTCAT700.001483466410.85827210
CTGTGTG750.002633645710.1343879
TTCAAGA856.561378E-410.05806352
AGACAGT900.00110642939.5009876
CTGGAGG800.00449864059.4992824
CTGACCC950.00181494568.999324
TATATCT850.00740747678.94054
CTGTAGA1050.00448411148.1437031
TATCAAC4400.07.9880332
ATCAACG4700.07.8823833
TCAACGC4850.07.6385984
Produced by FastQC (version 0.11.5)