##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064000_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278585
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286156828257084	32.0	32.0	32.0	32.0	32.0
2	30.91294218999587	32.0	32.0	32.0	32.0	32.0
3	30.90152018235009	32.0	32.0	32.0	32.0	32.0
4	30.93945833408116	32.0	32.0	32.0	32.0	32.0
5	30.870334009368776	32.0	32.0	32.0	32.0	32.0
6	34.52907730136224	36.0	36.0	36.0	32.0	36.0
7	34.466819821598435	36.0	36.0	36.0	32.0	36.0
8	34.45466195236642	36.0	36.0	36.0	32.0	36.0
9	34.56999838469408	36.0	36.0	36.0	32.0	36.0
10	34.30194016188955	36.0	36.0	36.0	32.0	36.0
11	34.542297682933395	36.0	36.0	36.0	32.0	36.0
12	34.401076870614	36.0	36.0	36.0	32.0	36.0
13	34.435314177001636	36.0	36.0	36.0	32.0	36.0
14	34.35212233250175	36.0	36.0	36.0	32.0	36.0
15	34.30818242188201	36.0	36.0	36.0	32.0	36.0
16	34.30174273561032	36.0	36.0	36.0	32.0	36.0
17	34.24691925265179	36.0	36.0	36.0	32.0	36.0
18	34.22989392824452	36.0	36.0	36.0	32.0	36.0
19	34.27324156002656	36.0	36.0	36.0	32.0	36.0
20	34.2360069637633	36.0	36.0	36.0	32.0	36.0
21	34.251729274727644	36.0	36.0	36.0	32.0	36.0
22	34.225331586409894	36.0	36.0	36.0	32.0	36.0
23	34.14981423981909	36.0	36.0	36.0	32.0	36.0
24	34.160015794102335	36.0	36.0	36.0	32.0	36.0
25	33.75105264102518	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	3.0
5	1.0
6	13.0
7	1.0
8	15.0
9	17.0
10	29.0
11	11.0
12	25.0
13	12.0
14	23.0
15	52.0
16	78.0
17	101.0
18	99.0
19	155.0
20	249.0
21	319.0
22	535.0
23	871.0
24	1319.0
25	1938.0
26	2854.0
27	3900.0
28	5305.0
29	7065.0
30	9531.0
31	12884.0
32	18174.0
33	25876.0
34	57508.0
35	129621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62088344183942	18.090412562527156	12.749255778712218	25.53944821692121
2	15.73169637248039	20.25523665930752	38.544325381867786	25.4687415863443
3	18.11916231719662	23.9936391244104	29.768323413909208	28.118875144483773
4	12.982905798818267	16.623949112264604	36.90473626397295	33.48840882494418
5	14.396025315637756	36.43843582959689	34.44570887016582	14.719829984599533
6	32.358864815379924	36.6824032023551	17.416934427112317	13.541797555152668
7	29.11040180382667	31.291940585741006	21.446651754458404	18.15100585597392
8	27.15853881868813	34.41489189800601	19.665909001138292	18.76066028216757
9	26.916750466886942	15.379974141646317	19.153498060623473	38.54977733084327
10	15.631801627721547	27.90831316040887	32.739040175845645	23.720845036023935
11	34.957314379505156	22.593030230110873	23.23196770474552	19.217687685638452
12	23.71576142004353	24.82130927324057	29.464035572923773	21.998893733792123
13	28.833326748249867	20.85938931133197	25.67464180139149	24.63264213902668
14	22.894829481367598	20.077159052538562	26.498818187696223	30.52919327839762
15	24.85892955277705	28.115313192556222	22.511646618080725	24.514110636586008
16	24.250446301890452	27.299667024666036	24.371495587268633	24.07839108617488
17	23.123228825826008	27.501194251787787	25.763872177345494	23.61170474504071
18	23.474512339691778	26.84987606423106	26.9245967597083	22.75101483636886
19	24.30453774750697	25.98068799034399	26.982211110153177	22.732563151995862
20	24.67479747813123	25.520809009753382	26.49327322041205	23.31112029170334
21	24.887380105614827	25.523942953622875	26.19930308582103	23.389373854941265
22	25.096631893554044	25.538839555134064	25.945843032445325	23.41868551886657
23	24.287146177695227	25.754347896376185	26.131566751570844	23.82693917435774
24	23.870114847558458	25.76615930653198	26.428948625744965	23.9347772201646
25	24.430777189415224	25.85841458245019	26.136829021202608	23.57397920693198
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	15.0
2	44.0
3	73.0
4	73.0
5	73.0
6	154.0
7	235.0
8	235.0
9	235.0
10	367.5
11	500.0
12	500.0
13	500.0
14	716.0
15	932.0
16	932.0
17	932.0
18	1687.0
19	2442.0
20	2442.0
21	2442.0
22	3511.5
23	4581.0
24	4581.0
25	4581.0
26	6726.5
27	8872.0
28	8872.0
29	8872.0
30	11684.0
31	14496.0
32	14496.0
33	14496.0
34	17792.5
35	21089.0
36	21089.0
37	21089.0
38	24764.5
39	28440.0
40	28440.0
41	28440.0
42	30991.0
43	33542.0
44	33542.0
45	33542.0
46	35651.5
47	37761.0
48	37761.0
49	37761.0
50	37426.5
51	37092.0
52	37092.0
53	37092.0
54	34326.0
55	31560.0
56	31560.0
57	31560.0
58	27329.0
59	23098.0
60	23098.0
61	23098.0
62	19693.0
63	16288.0
64	16288.0
65	16288.0
66	12675.0
67	9062.0
68	9062.0
69	9062.0
70	6761.5
71	4461.0
72	4461.0
73	4461.0
74	3233.5
75	2006.0
76	2006.0
77	2006.0
78	1524.0
79	1042.0
80	1042.0
81	1042.0
82	709.0
83	376.0
84	376.0
85	376.0
86	277.5
87	179.0
88	179.0
89	179.0
90	113.5
91	48.0
92	48.0
93	48.0
94	38.0
95	28.0
96	28.0
97	28.0
98	197.5
99	367.0
100	367.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0380494283611824
2	0.007179137426638189
3	0.002512698099323366
4	0.003948525584651004
5	0.007897051169302009
6	0.014358274853276377
7	0.023691153507906022
8	0.03517777339052713
9	0.052048746343126874
10	0.0570741425417736
11	0.055279358185114064
12	0.061381624997756526
13	0.06389432309707989
14	0.07143241739504999
15	0.06389432309707989
16	0.06676597806773515
17	0.06030475438376079
18	0.07717572733636054
19	0.07502198610836908
20	0.07897051169302008
21	0.07717572733636054
22	0.07502198610836908
23	0.083277994149003
24	0.07753468420769245
25	0.08004738230701582
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	278585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.915157729891575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.91677014340906	24.85507325910275
2	17.215595740511837	14.7762001323623
3	8.756763288104855	11.273936331370317
4	5.043632710652594	8.65793173237187
5	3.231247526365454	6.933474862914775
6	2.09369379664729	5.391071971272839
7	1.4354389770287184	4.312146307757516
8	0.9845578334971242	3.380196377498353
9	0.7557945039073465	2.9191536311912043
>10	2.5338271643540864	15.801040657732957
>50	0.02346135473348508	0.6681153924852818
>100	0.008379055261958958	0.7623275737722268
>500	8.379055261958957E-4	0.26933177016764054
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	749	0.2688586966276002	No Hit
TATCAACGCAGAGTACTTTTTTTTT	452	0.16224850584202308	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	337	0.12096846563885348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTAAC	30	7.6956104E-4	19.001976	1
CCCTTAA	30	7.6956104E-4	19.001976	1
TTATATA	25	0.0060225567	18.998564	4
GGTATCA	60	7.6091965E-8	17.418478	1
TGTTACA	40	0.005267361	14.248922	2
GTATCAA	265	0.0	13.624058	1
GTACAAG	60	4.069742E-4	12.667983	1
TGCACTG	60	4.075915E-4	12.6657095	5
AAGGTTG	55	0.0030593178	12.089995	5
TGCTCAT	70	0.0014834664	10.858272	10
CTGTGTG	75	0.0026336457	10.134387	9
TTCAAGA	85	6.561378E-4	10.0580635	2
AGACAGT	90	0.0011064293	9.500987	6
CTGGAGG	80	0.0044986405	9.499282	4
CTGACCC	95	0.0018149456	8.99932	4
TATATCT	85	0.0074074767	8.9405	4
CTGTAGA	105	0.0044841114	8.143703	1
TATCAAC	440	0.0	7.988033	2
ATCAACG	470	0.0	7.882383	3
TCAACGC	485	0.0	7.638598	4
>>END_MODULE