FastQCFastQC Report
Thu 2 Feb 2017
SRR4064000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278585
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC254.4348788E-543.99874517
GTACTAT250.002350024935.1989941
GGTATCA1350.034.2212451
AGGCCGT353.2120955E-431.4276736
GGCGGAC407.0122693E-427.5041521
TAGGACC407.0196344E-427.4992144
GTATCAA4300.027.1155051
CCGTGCG508.320442E-526.3992469
CGTGCGG450.001395749524.44374710
GGCCGTG551.5896371E-423.9993177
GTATTTA602.8656522E-421.9993721
TGTATAC500.002575928621.9993723
GCCGTGC500.002575928621.9993728
TATCCTC703.2046206E-521.9993725
TCAACGC5350.020.9713634
AGTACCT856.087159E-620.7052925
ATCAACG5550.020.6120223
TATCAAC5700.020.4555552
CAGTACC654.920017E-420.3071144
TTACACA654.920017E-420.3071144
Produced by FastQC (version 0.11.5)