##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278585
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.371272681587307	32.0	32.0	32.0	32.0	32.0
2	31.484286662957444	32.0	32.0	32.0	32.0	32.0
3	31.519421361523413	32.0	32.0	32.0	32.0	32.0
4	31.58417000197426	32.0	32.0	32.0	32.0	32.0
5	31.567614911068436	32.0	32.0	32.0	32.0	32.0
6	35.14789023098875	36.0	36.0	36.0	36.0	36.0
7	35.16261464185078	36.0	36.0	36.0	36.0	36.0
8	35.11517490173556	36.0	36.0	36.0	36.0	36.0
9	35.20264910171043	36.0	36.0	36.0	36.0	36.0
10	35.08058940718273	36.0	36.0	36.0	36.0	36.0
11	35.18573146436456	36.0	36.0	36.0	36.0	36.0
12	35.11226735107777	36.0	36.0	36.0	36.0	36.0
13	35.15502629359082	36.0	36.0	36.0	36.0	36.0
14	35.10878187985713	36.0	36.0	36.0	36.0	36.0
15	35.097072706714286	36.0	36.0	36.0	36.0	36.0
16	35.119188039557045	36.0	36.0	36.0	36.0	36.0
17	35.07794389504101	36.0	36.0	36.0	36.0	36.0
18	35.07700342803812	36.0	36.0	36.0	36.0	36.0
19	35.07870488360823	36.0	36.0	36.0	36.0	36.0
20	35.07600552793582	36.0	36.0	36.0	36.0	36.0
21	35.076640881598074	36.0	36.0	36.0	36.0	36.0
22	35.05194464885044	36.0	36.0	36.0	36.0	36.0
23	35.01806629933414	36.0	36.0	36.0	36.0	36.0
24	34.999321571513185	36.0	36.0	36.0	36.0	36.0
25	34.96240644686541	36.0	36.0	36.0	36.0	36.0
26	34.93037313566775	36.0	36.0	36.0	36.0	36.0
27	34.91925983093132	36.0	36.0	36.0	36.0	36.0
28	34.89100633558878	36.0	36.0	36.0	36.0	36.0
29	34.87713624208052	36.0	36.0	36.0	32.0	36.0
30	34.876425507475275	36.0	36.0	36.0	32.0	36.0
31	34.865208105246154	36.0	36.0	36.0	32.0	36.0
32	34.832036900766376	36.0	36.0	36.0	32.0	36.0
33	34.824782382396755	36.0	36.0	36.0	32.0	36.0
34	34.80869393542366	36.0	36.0	36.0	32.0	36.0
35	34.77719546996428	36.0	36.0	36.0	32.0	36.0
36	34.73973114130337	36.0	36.0	36.0	32.0	36.0
37	34.732857117217364	36.0	36.0	36.0	32.0	36.0
38	34.69356569808138	36.0	36.0	36.0	32.0	36.0
39	34.70389647683831	36.0	36.0	36.0	32.0	36.0
40	34.7038139167579	36.0	36.0	36.0	32.0	36.0
41	34.68981459877596	36.0	36.0	36.0	32.0	36.0
42	34.643993754150436	36.0	36.0	36.0	32.0	36.0
43	34.61372651075973	36.0	36.0	36.0	32.0	36.0
44	34.5903512392986	36.0	36.0	36.0	32.0	36.0
45	34.56473248739164	36.0	36.0	36.0	32.0	36.0
46	34.54011881472441	36.0	36.0	36.0	32.0	36.0
47	34.499868980741965	36.0	36.0	36.0	32.0	36.0
48	34.48431537950715	36.0	36.0	36.0	32.0	36.0
49	34.447884846635674	36.0	36.0	36.0	32.0	36.0
50	33.91786348870183	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	0.0
21	6.0
22	22.0
23	71.0
24	200.0
25	438.0
26	879.0
27	1755.0
28	2997.0
29	4448.0
30	6453.0
31	9079.0
32	12912.0
33	20214.0
34	41651.0
35	177458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47125974641375	19.036917907554457	13.386869803707585	26.104952542324206
2	14.904301754587484	20.975720070068633	38.171969675214655	25.948008500129227
3	17.31901329581024	24.773497401142922	30.10510294920024	27.802386353846597
4	12.582874167668754	17.303157025683362	36.774054597340125	33.33991420930775
5	13.89809214422887	37.17500942261787	34.224383940269576	14.702514492883681
6	32.36474456537346	37.05094334204405	17.271989776871607	13.312322315710881
7	28.917924511369957	31.789220525153905	21.203941346447223	18.088913617028915
8	27.172317246082883	34.32309707988585	19.87221135380584	18.632374320225427
9	26.86148732325607	15.090763548124245	19.17108489092939	38.87666423769029
10	15.468502650953589	27.924933322325085	32.96802702285528	23.63853700386605
11	35.14797997020658	22.48146885151749	23.268661270348368	19.101889907927564
12	23.325017499147478	24.855250641635408	29.51163917655294	22.308092682664178
13	29.03515289877702	20.764368249318874	25.707239853113794	24.493238998790307
14	22.813944806593344	20.081196335755106	26.63325962725785	30.471599230393704
15	24.7281721032246	28.242819758989434	22.41714140076173	24.611866737024236
16	24.141904358501268	27.41670316100825	24.478968490426517	23.962423990063968
17	22.992264479422797	27.570400416389973	25.717465046574656	23.719870057612578
18	23.49803471112946	26.9835059317623	26.68054633235817	22.837913024750076
19	24.010625123391424	26.24836225927455	27.125293895938402	22.615718721395623
20	24.72584472132183	25.604577450885373	26.440952965543485	23.228624862249312
21	24.815406428917566	25.38758368182063	26.329127555324227	23.467882333937577
22	25.17149164527882	25.521833551698762	25.854586571423443	23.45208823159897
23	24.26548450203708	25.68695371251144	26.062422599924616	23.98513918552686
24	23.929758563008377	25.689743055904547	26.35417937985943	24.026319001227645
25	24.130046163013592	25.888979029212646	26.329430177544527	23.651544630229235
26	24.0654918530059	25.80976346973281	26.49217967541255	23.632565001848736
27	23.978159574391704	26.422104635885212	25.84486261783563	23.754873171887454
28	23.66140658204583	25.887011084946298	26.40177186835908	24.049810464648786
29	23.956392667018456	26.375472490550187	26.03265883400281	23.63547600842855
30	24.026391267046453	26.209988620740404	25.9824034288545	23.781216683358643
31	23.78456578942645	25.68862339937465	26.354899968768287	24.171910842430616
32	24.08417183287684	26.12923721968748	25.737598403291056	24.048992544144625
33	23.400390568909565	26.353349989948594	26.389247867666064	23.857011573475777
34	24.122940844949078	25.417036109027073	26.620670344936514	23.83935270108733
35	23.971454520000144	26.36292820912292	25.99821231777667	23.667404953100267
36	23.96127253537812	25.852054479792653	26.122730307795035	24.0639426770342
37	24.562411154028403	25.815588070588575	25.744870266932786	23.87713050845024
38	23.766940101595736	25.969377681247867	26.172928147044573	24.090754070111828
39	23.939232945163187	25.570768059962955	26.56835575721896	23.921643237654898
40	24.322267532019985	25.66588650852909	26.085879616334502	23.92596634311642
41	24.190253971102933	25.620748451942926	26.540069999102577	23.648927577851566
42	24.169520486477563	25.379970851551114	26.371089907888027	24.079418754083296
43	23.781404915661266	25.337157502898638	26.455332239687845	24.42610534175225
44	23.419400461625166	25.5850183609076	26.45944985479986	24.536131322667377
45	23.074796917234124	26.025823728107806	26.450475804164675	24.44890355049339
46	23.4762850035358	25.61212438841406	26.535381346045465	24.376209262004675
47	23.618604550985904	25.740459549783363	26.603057682436095	24.03787821679464
48	23.47691865891306	25.980687773709526	26.53995261684256	24.002440950534858
49	23.7860888855386	25.45991679319987	26.32429114482937	24.429703176432156
50	23.14443555016315	25.55917309507827	26.731543070058617	24.564848284699963
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.5
2	5.0
3	26.5
4	48.0
5	51.5
6	55.0
7	59.5
8	64.0
9	83.5
10	103.0
11	161.0
12	219.0
13	353.0
14	487.0
15	611.0
16	735.0
17	712.5
18	690.0
19	632.5
20	575.0
21	658.0
22	741.0
23	1003.0
24	1265.0
25	1643.0
26	2021.0
27	2553.5
28	3086.0
29	3607.0
30	4128.0
31	5162.0
32	6196.0
33	7023.0
34	7850.0
35	8862.5
36	9875.0
37	10949.5
38	12024.0
39	13691.0
40	15358.0
41	16367.0
42	17376.0
43	18322.0
44	19268.0
45	20697.5
46	22127.0
47	22255.0
48	22383.0
49	21882.5
50	21382.0
51	21090.0
52	20798.0
53	20483.5
54	20169.0
55	19114.0
56	18059.0
57	16341.5
58	14624.0
59	12974.0
60	11324.0
61	10022.0
62	8720.0
63	7292.0
64	5864.0
65	4868.5
66	3873.0
67	3203.5
68	2534.0
69	2066.5
70	1599.0
71	1273.0
72	947.0
73	853.0
74	759.0
75	642.0
76	525.0
77	414.0
78	303.0
79	239.5
80	176.0
81	148.0
82	120.0
83	98.0
84	76.0
85	56.5
86	37.0
87	23.0
88	9.0
89	5.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007538094297970099
2	3.5895687133190947E-4
3	3.5895687133190947E-4
4	0.0
5	0.0
6	3.5895687133190947E-4
7	0.0
8	0.0
9	0.0014358274853276379
10	0.0021537412279914567
11	0.0
12	0.0
13	7.179137426638189E-4
14	3.5895687133190947E-4
15	0.0028716549706552757
16	0.0010768706139957283
17	0.0
18	0.0
19	0.0
20	7.179137426638189E-4
21	0.0
22	0.0
23	0.0
24	0.0010768706139957283
25	0.002512698099323366
26	0.0057433099413105515
27	0.0068201805553062805
28	0.0032306118419871857
29	0.0028716549706552757
30	0.0028716549706552757
31	0.007897051169302009
32	0.004307482455982913
33	0.0061022668126424606
34	0.004307482455982913
35	0.004307482455982913
36	0.008256008040633917
37	0.004666439327314823
38	0.010768706139957285
39	0.004666439327314823
40	0.0032306118419871857
41	0.0035895687133190943
42	0.003948525584651004
43	0.0021537412279914567
44	0.0014358274853276379
45	0.0014358274853276379
46	0.0021537412279914567
47	0.0028716549706552757
48	0.0017947843566595472
49	7.179137426638189E-4
50	0.0014358274853276379
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	278585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.70588130460977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.66168358404772	28.79963274518938
2	16.295661327381783	15.222064474736266
3	7.917071545353585	11.09321411632164
4	4.4711797539932165	8.35321563526326
5	2.867132963078316	6.695598592903497
6	1.8316300588238406	5.132873767282903
7	1.307406970824858	4.274451635731565
8	0.8579565588532077	3.205729376184749
9	0.6657718969100004	2.798591687372104
>10	2.1121626016236963	13.988666097465602
>50	0.010028475526666778	0.27418759028828726
>100	0.0023142635830769487	0.16177428126076343
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	3.5895687133190947E-4	0.0
22	0.0	0.0	0.0	3.5895687133190947E-4	0.0
23	0.0	0.0	0.0	3.5895687133190947E-4	0.0
24	0.0	0.0	0.0	3.5895687133190947E-4	0.0
25	0.0	0.0	0.0	3.5895687133190947E-4	0.0
26	0.0	0.0	0.0	7.179137426638189E-4	0.0
27	0.0	0.0	0.0	0.0014358274853276379	0.0
28	0.0	0.0	0.0	0.0064612236839743705	0.0
29	0.0	0.0	0.0	0.016870972952599744	0.0
30	0.0	0.0	0.0	0.03697255774718668	0.0
31	0.0	0.0	0.0	0.09548252777428792	0.0
32	0.0	0.0	0.0	0.15399249780138916	0.0
33	0.0	0.0	0.0	0.20783602850117558	0.0
34	0.0	0.0	0.0	0.28214010086688085	0.0
35	0.0	0.0	0.0	0.35572625948992226	0.0
36	0.0	0.0	0.0	0.4551573128488612	0.0
37	0.0	0.0	0.0	0.6303282660588331	0.0
38	0.0	0.0	0.0	0.864368146167238	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGGC	25	4.4348788E-5	43.998745	17
GTACTAT	25	0.0023500249	35.198994	1
GGTATCA	135	0.0	34.221245	1
AGGCCGT	35	3.2120955E-4	31.427673	6
GGCGGAC	40	7.0122693E-4	27.50415	21
TAGGACC	40	7.0196344E-4	27.499214	4
GTATCAA	430	0.0	27.115505	1
CCGTGCG	50	8.320442E-5	26.399246	9
CGTGCGG	45	0.0013957495	24.443747	10
GGCCGTG	55	1.5896371E-4	23.999317	7
GTATTTA	60	2.8656522E-4	21.999372	1
TGTATAC	50	0.0025759286	21.999372	3
GCCGTGC	50	0.0025759286	21.999372	8
TATCCTC	70	3.2046206E-5	21.999372	5
TCAACGC	535	0.0	20.971363	4
AGTACCT	85	6.087159E-6	20.705292	5
ATCAACG	555	0.0	20.612022	3
TATCAAC	570	0.0	20.455555	2
CAGTACC	65	4.920017E-4	20.307114	4
TTACACA	65	4.920017E-4	20.307114	4
>>END_MODULE