FastQCFastQC Report
Thu 2 Feb 2017
SRR4063999_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063999_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288214
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18020.13988359076985657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC801.2927194E-714.2455723
TCTAATC551.9649125E-413.81388953
GCGTTAT1001.009721E-813.2969011
TAAGACT1153.783498E-1013.2148254
CTCTTAA1850.012.83484651
GTATCAA15250.012.3938321
TTATACT1001.4453144E-712.3476014
TTATCGG853.9401148E-612.29459611
GCATAAG550.003073312812.0880921
ACCGGCG802.8631865E-511.8759238
ACCGTCG2400.011.8759238
CGGTCCA2500.011.78045810
TCCATTA2000.011.4008868
GATATAC2350.011.3165111
CCGACCA1850.011.2981759
TACCGTC2550.011.1760377
TGATACT855.3315187E-511.17434
CCGTCGT2300.011.153049
ATACCGT2900.011.1366336
ATTATAC600.00588833311.079893
Produced by FastQC (version 0.11.5)