##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063999_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1288214
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.72480659269345	32.0	21.0	32.0	14.0	32.0
2	24.563092001794733	32.0	14.0	32.0	14.0	32.0
3	24.540837159043452	32.0	14.0	32.0	14.0	32.0
4	24.438641405853375	32.0	14.0	32.0	14.0	32.0
5	23.659596154055148	32.0	14.0	32.0	14.0	32.0
6	27.65412967100187	36.0	14.0	36.0	14.0	36.0
7	27.296491887217496	32.0	14.0	36.0	14.0	36.0
8	26.872115192041075	32.0	14.0	36.0	14.0	36.0
9	27.88431269959805	32.0	14.0	36.0	14.0	36.0
10	25.395289913011347	32.0	14.0	36.0	14.0	36.0
11	28.40664128786056	36.0	14.0	36.0	14.0	36.0
12	26.690526574000902	32.0	14.0	36.0	14.0	36.0
13	27.51357848928827	32.0	14.0	36.0	14.0	36.0
14	26.655776136573582	32.0	14.0	36.0	14.0	36.0
15	26.33453991339948	32.0	14.0	36.0	14.0	36.0
16	26.50248095425139	32.0	14.0	36.0	14.0	36.0
17	26.225389570366413	32.0	14.0	36.0	14.0	36.0
18	26.32361703878393	32.0	14.0	36.0	14.0	36.0
19	26.465555412377135	32.0	14.0	36.0	14.0	36.0
20	26.488368392208127	32.0	14.0	36.0	14.0	36.0
21	26.548588976676236	32.0	14.0	36.0	14.0	36.0
22	26.415035855843826	32.0	14.0	36.0	14.0	36.0
23	26.206353136978795	32.0	14.0	36.0	14.0	36.0
24	26.216033981931574	32.0	14.0	36.0	14.0	36.0
25	25.61018899033856	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
2	2.0
3	10.0
4	30.0
5	53.0
6	79.0
7	113.0
8	104.0
9	89.0
10	82.0
11	62.0
12	47.0
13	52.0
14	447.0
15	1567.0
16	4375.0
17	11271.0
18	23408.0
19	35941.0
20	53698.0
21	71204.0
22	88033.0
23	103398.0
24	110904.0
25	113977.0
26	111403.0
27	102337.0
28	94910.0
29	85419.0
30	74600.0
31	64535.0
32	53263.0
33	38531.0
34	30023.0
35	14247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.82855234752357	16.779629428300744	11.345380278627673	25.046437945548018
2	18.756943199100093	18.642746739267977	37.084582235812974	25.515727825818956
3	21.017200195934894	22.62597783078957	28.187221846585466	28.169600126690074
4	14.057181095723879	14.252577139516811	35.92119880169514	35.769042963064166
5	16.77276658380522	35.311475715281645	32.82796131338241	15.087796387530725
6	36.18959775964646	34.4090229043075	16.55459464243706	12.846784693608985
7	32.44516017321396	29.255051103085904	20.356910194760875	17.942878528939264
8	30.346207190038875	32.06800204391307	18.87272603022693	18.71306473582113
9	29.569981414299573	13.534397525841735	18.34073887673401	38.55488218312468
10	18.397699637034165	24.978076801488836	30.857843689342257	25.766379872134742
11	39.035304041327464	20.049352491275545	22.366993327238706	18.54835014015828
12	27.440519699633125	22.150853025457547	28.242316245895665	22.166311029013663
13	31.800806462262425	18.62102876371712	24.596788288508726	24.98137648551172
14	25.810912006065333	18.979566788574058	24.551780603985346	30.657740601375266
15	27.406977033207397	26.550305105388745	21.475986155299264	24.566731706104594
16	27.84529494060173	24.431710047252952	23.662907714978402	24.06008729716692
17	26.350818103292923	24.696357218759516	24.658994164838646	24.293830513108915
18	26.98691639028794	23.59081808880322	26.00049876357907	23.421766757329763
19	28.380943058659753	23.29477788472519	24.45944297747812	23.864836079136943
20	28.469577539633335	23.212401742111012	24.185929796593157	24.132090921662495
21	29.421896206545885	22.773232566107126	23.54965191645995	24.255219310887043
22	28.658984245416924	23.219267531139753	24.05131834606011	24.070429877383212
23	27.013930433296302	23.133220936827467	24.754527585054888	25.098321044821343
24	27.776435500649082	23.56310096574034	24.563723247143603	24.09674028646697
25	29.145846151933824	22.12249610003791	24.28984953293681	24.441808215091456
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	68.0
2	216.0
3	364.0
4	364.0
5	364.0
6	904.0
7	1444.0
8	1444.0
9	1444.0
10	1630.5
11	1817.0
12	1817.0
13	1817.0
14	2264.5
15	2712.0
16	2712.0
17	2712.0
18	4605.0
19	6498.0
20	6498.0
21	6498.0
22	11211.0
23	15924.0
24	15924.0
25	15924.0
26	24575.0
27	33226.0
28	33226.0
29	33226.0
30	43406.0
31	53586.0
32	53586.0
33	53586.0
34	65011.5
35	76437.0
36	76437.0
37	76437.0
38	86629.0
39	96821.0
40	96821.0
41	96821.0
42	109841.0
43	122861.0
44	122861.0
45	122861.0
46	137220.5
47	151580.0
48	151580.0
49	151580.0
50	156873.0
51	162166.0
52	162166.0
53	162166.0
54	155046.5
55	147927.0
56	147927.0
57	147927.0
58	137760.5
59	127594.0
60	127594.0
61	127594.0
62	115463.5
63	103333.0
64	103333.0
65	103333.0
66	88793.0
67	74253.0
68	74253.0
69	74253.0
70	59776.5
71	45300.0
72	45300.0
73	45300.0
74	35972.5
75	26645.0
76	26645.0
77	26645.0
78	21783.0
79	16921.0
80	16921.0
81	16921.0
82	12985.5
83	9050.0
84	9050.0
85	9050.0
86	7090.0
87	5130.0
88	5130.0
89	5130.0
90	4103.5
91	3077.0
92	3077.0
93	3077.0
94	2341.5
95	1606.0
96	1606.0
97	1606.0
98	1740.0
99	1874.0
100	1874.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0367951287596626
2	0.006443028875637122
3	0.0024064324716235037
4	0.004735238089323668
5	0.009315222470800659
6	0.015758251346437782
7	0.025383981232931795
8	0.03788190471458935
9	0.05162185785902031
10	0.0593845432513542
11	0.05752149875719407
12	0.0663709601044547
13	0.0669919749358414
14	0.06970891482315826
15	0.061325214599437676
16	0.06885501943000154
17	0.06567231841914464
18	0.08003328639496232
19	0.07584143628310204
20	0.08073192808027238
21	0.07941227156357561
22	0.08018854010280901
23	0.08570004673136607
24	0.07879125673218891
25	0.07933464470965228
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1288214.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.39681508588892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.05336164333586	58.67119317280921
2	7.987559790578379	10.766721803861852
3	2.151488297810491	4.350103769009627
4	0.930294977229342	2.507956742226288
5	0.4784989989033192	1.6124654273934984
6	0.2951122365401645	1.1933774901408347
7	0.2005521083696326	0.9461601354011298
8	0.1318633625315271	0.7109736528912693
9	0.10955550055492358	0.6645322631287967
>10	0.5521162262842627	6.8147782794297544
>50	0.051359881748836704	2.414636653137904
>100	0.053630012722816665	7.059789950590423
>500	0.004370708857495372	2.0518124026798774
>1k	2.3625453283758764E-4	0.23549825729966928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1802	0.13988359076985657	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5525370784667765E-4
2	0.0	0.0	0.0	0.0	1.5525370784667765E-4
3	0.0	0.0	0.0	0.0	1.5525370784667765E-4
4	0.0	0.0	0.0	0.0	1.5525370784667765E-4
5	0.0	0.0	0.0	0.0	1.5525370784667765E-4
6	7.762685392333882E-5	0.0	0.0	0.0	1.5525370784667765E-4
7	7.762685392333882E-5	0.0	0.0	0.0	1.5525370784667765E-4
8	7.762685392333882E-5	0.0	0.0	0.0	1.5525370784667765E-4
9	7.762685392333882E-5	0.0	0.0	0.0	1.5525370784667765E-4
10	7.762685392333882E-5	0.0	0.0	0.0	1.5525370784667765E-4
11	7.762685392333882E-5	0.0	0.0	0.0	2.3288056177001647E-4
12	7.762685392333882E-5	0.0	0.0	0.0	4.6576112354003294E-4
13	7.762685392333882E-5	0.0	0.0	0.0	6.210148313867106E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	80	1.2927194E-7	14.245572	3
TCTAATC	55	1.9649125E-4	13.8138895	3
GCGTTAT	100	1.009721E-8	13.296901	1
TAAGACT	115	3.783498E-10	13.214825	4
CTCTTAA	185	0.0	12.8348465	1
GTATCAA	1525	0.0	12.393832	1
TTATACT	100	1.4453144E-7	12.347601	4
TTATCGG	85	3.9401148E-6	12.294596	11
GCATAAG	55	0.0030733128	12.088092	1
ACCGGCG	80	2.8631865E-5	11.875923	8
ACCGTCG	240	0.0	11.875923	8
CGGTCCA	250	0.0	11.780458	10
TCCATTA	200	0.0	11.400886	8
GATATAC	235	0.0	11.316511	1
CCGACCA	185	0.0	11.298175	9
TACCGTC	255	0.0	11.176037	7
TGATACT	85	5.3315187E-5	11.1743	4
CCGTCGT	230	0.0	11.15304	9
ATACCGT	290	0.0	11.136633	6
ATTATAC	60	0.005888333	11.07989	3
>>END_MODULE