FastQCFastQC Report
Thu 2 Feb 2017
SRR4063999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288214
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13990.10859996863875103No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13710.10642641672889754No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13090.10161355178565051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT655.931888E-727.07682629
GTATCAA19300.025.0766581
CTAGCCG703.2175798E-521.9982154
GATATAC3850.021.7133661
CGATTTT856.1100236E-620.70741831
GTATTAC1352.5993359E-919.5547281
ATACACT4700.019.1899324
GTCCTAA4450.018.785721
TATACAC5350.018.503173
CGTATGT1203.159912E-718.33469233
AACGCAG26900.018.2364396
AATGCCG1852.1827873E-1117.8363918
CGGATCG1002.4816367E-517.60130526
GGATCGA1002.4824647E-517.60062227
GCATATA1254.8552465E-717.5992551
AACGTAT1154.2703105E-617.21866631
GTATATA1551.4400939E-817.0315361
TCAACGC28750.016.9099314
GTAGGAC7550.016.899293
GGTATCA9150.016.829891
Produced by FastQC (version 0.11.5)