##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063999_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1288214
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34709605702158	32.0	32.0	32.0	32.0	32.0
2	31.407057367797588	32.0	32.0	32.0	32.0	32.0
3	31.47956550697322	32.0	32.0	32.0	32.0	32.0
4	31.557151994932518	32.0	32.0	32.0	32.0	32.0
5	31.518081623084363	32.0	32.0	32.0	32.0	32.0
6	35.10201954023167	36.0	36.0	36.0	36.0	36.0
7	35.11823811882187	36.0	36.0	36.0	36.0	36.0
8	35.061396631305044	36.0	36.0	36.0	36.0	36.0
9	35.175222439749916	36.0	36.0	36.0	36.0	36.0
10	35.029603777012206	36.0	36.0	36.0	36.0	36.0
11	35.16563008941061	36.0	36.0	36.0	36.0	36.0
12	35.07174506720157	36.0	36.0	36.0	36.0	36.0
13	35.12434735222564	36.0	36.0	36.0	36.0	36.0
14	35.06212710000047	36.0	36.0	36.0	36.0	36.0
15	35.03109809395023	36.0	36.0	36.0	36.0	36.0
16	35.049599678314316	36.0	36.0	36.0	36.0	36.0
17	35.01266249241198	36.0	36.0	36.0	36.0	36.0
18	35.00893407461804	36.0	36.0	36.0	36.0	36.0
19	35.00496190850278	36.0	36.0	36.0	36.0	36.0
20	34.9957895194432	36.0	36.0	36.0	36.0	36.0
21	34.98832181609577	36.0	36.0	36.0	36.0	36.0
22	34.974983970054666	36.0	36.0	36.0	36.0	36.0
23	34.92949385738705	36.0	36.0	36.0	32.0	36.0
24	34.895452929404584	36.0	36.0	36.0	32.0	36.0
25	34.865685359730605	36.0	36.0	36.0	32.0	36.0
26	34.80557655793214	36.0	36.0	36.0	32.0	36.0
27	34.80030569455075	36.0	36.0	36.0	32.0	36.0
28	34.77886981510836	36.0	36.0	36.0	32.0	36.0
29	34.7482103128828	36.0	36.0	36.0	32.0	36.0
30	34.73209032039708	36.0	36.0	36.0	32.0	36.0
31	34.71967778645473	36.0	36.0	36.0	32.0	36.0
32	34.68697592170245	36.0	36.0	36.0	32.0	36.0
33	34.65534453126577	36.0	36.0	36.0	32.0	36.0
34	34.648875885528334	36.0	36.0	36.0	32.0	36.0
35	34.615079482135734	36.0	36.0	36.0	32.0	36.0
36	34.58641343751892	36.0	36.0	36.0	32.0	36.0
37	34.57370204018898	36.0	36.0	36.0	32.0	36.0
38	34.532600949842184	36.0	36.0	36.0	32.0	36.0
39	34.518109568751775	36.0	36.0	36.0	32.0	36.0
40	34.50726276845307	36.0	36.0	36.0	32.0	36.0
41	34.486174657316255	36.0	36.0	36.0	32.0	36.0
42	34.424980632099945	36.0	36.0	36.0	32.0	36.0
43	34.41930222773546	36.0	36.0	36.0	32.0	36.0
44	34.34452117427694	36.0	36.0	36.0	32.0	36.0
45	34.33205042019416	36.0	36.0	36.0	32.0	36.0
46	34.291701534061886	36.0	36.0	36.0	32.0	36.0
47	34.27070114127001	36.0	36.0	36.0	32.0	36.0
48	34.21931604531545	36.0	36.0	36.0	32.0	36.0
49	34.20094953167719	36.0	36.0	36.0	32.0	36.0
50	33.61054607386661	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	6.0
20	11.0
21	24.0
22	133.0
23	351.0
24	977.0
25	2255.0
26	4686.0
27	8558.0
28	14409.0
29	22240.0
30	32626.0
31	46759.0
32	67658.0
33	108784.0
34	226282.0
35	752451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04317616324883	18.33428435014498	12.183961586684212	26.438577899921977
2	15.61895964167333	20.515288656352613	37.642775634407435	26.222976067566623
3	18.013960435083668	24.542699493561617	29.103128988085814	28.3402110832689
4	12.25448566775396	16.38105159546473	36.22232020456229	35.142142532219026
5	14.15168597764036	37.07373153839347	33.540079520949156	15.234502963017013
6	33.87954175315592	35.989206762230495	16.85581743405987	13.275434050553711
7	29.845351781613928	30.650419883652873	21.021352042440153	18.482876292293053
8	27.617016751111812	33.555009924600974	19.482026652424718	19.345946671862496
9	27.482477474365375	14.019961201646641	18.590217815725676	39.90734350826231
10	15.989160101971429	26.84147852358788	31.510770033317705	25.65859134112299
11	37.19431491531292	20.781966957327707	23.032138318740767	18.991579808618607
12	24.977061193438956	23.487319614038086	28.998532847905235	22.537086344617727
13	29.523346718821685	19.553085635815872	25.346624116701378	25.57694352866107
14	23.432030932820787	20.13753958008432	25.1024094655301	31.32802002156479
15	24.89599498198592	27.96797600296237	22.010559255495636	25.12546975955608
16	25.384800496817267	25.69360347772085	24.346064275733582	24.575531749728302
17	23.867385387831526	26.13160546306747	25.334067165859086	24.666941983241912
18	24.689860108103577	24.85854391639866	26.76460108569339	23.68699488980437
19	25.823291645092855	24.62100599823166	25.278661215187242	24.277041141488247
20	25.877581120943955	24.520105573668687	24.898540599285827	24.70377270610154
21	26.76209077063283	24.121535707576534	24.325150945417455	24.79122257637318
22	25.950464675069014	24.607518017220766	24.805777309945878	24.636239997764342
23	24.22359139486123	24.436367757666275	25.67498856938365	25.665052278088847
24	25.12158372062875	24.948552523002903	25.34367535652267	24.586188399845675
25	24.988103490703644	24.563710562004296	25.528860970367017	24.919324976925044
26	24.3111520662915	25.54682023306672	25.8634810643965	24.27854663624528
27	25.133488079619294	25.022474439691646	24.94864648754396	24.895390993145103
28	24.376399278352757	25.003570973882205	25.601320949816948	25.018708797948086
29	24.638306692325667	24.753818768694053	25.28324911871583	25.32462542026445
30	24.375433753514663	24.978302620608705	25.742252211269594	24.904011414607034
31	25.37692674955264	24.60719733874693	24.704859426371094	25.311016485329336
32	24.820850091641844	25.068333531289035	24.68360080641718	25.42721557065194
33	24.37119417213443	24.74366458720838	25.49194352236841	25.39319771828878
34	25.164982490624276	24.823178982228235	25.618262228422246	24.393576298725243
35	25.61744424079685	24.74155257096679	25.423448558539885	24.217554629696476
36	24.386491041616367	25.218399174612983	25.245415228204138	25.14969455556651
37	25.27552716379165	25.06305471262655	24.77248679713112	24.888931326450685
38	24.7101942079211	25.021815008726005	25.29065976039202	24.97733102296088
39	25.541958978411692	24.583996751933583	24.64221973976653	25.231824529888186
40	25.89054420485447	24.86374112793856	25.196305154664785	24.049409512542184
41	24.844234462649652	25.024725304583146	25.714006244594977	24.41703398817222
42	25.83035369115951	25.59614838229883	24.94981314810128	23.623684778440378
43	24.800981217905672	24.773268022310287	25.395456433226336	25.0302943265577
44	24.530875324193428	25.256851242473005	24.91226109050091	25.300012342832655
45	24.75003803768992	25.448844741017673	24.95590733087201	24.845209890420403
46	24.76040099550223	24.90432341712506	24.97061777962015	25.36465780775256
47	25.27325373783167	24.7371485351426	25.24887826235464	24.740719464671088
48	25.635796666167256	25.345699032826758	24.28785572281043	24.730648578195556
49	24.953326672923414	25.432286220636556	24.56084377612639	25.053543330313637
50	24.870321924560816	25.909454350678086	24.375984909058445	24.844238815702653
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	17.5
2	20.0
3	59.5
4	99.0
5	120.5
6	142.0
7	151.5
8	161.0
9	215.5
10	270.0
11	414.0
12	558.0
13	1043.0
14	1528.0
15	2046.0
16	2564.0
17	2734.0
18	2904.0
19	2934.5
20	2965.0
21	3312.0
22	3659.0
23	4290.0
24	4921.0
25	6122.0
26	7323.0
27	9740.5
28	12158.0
29	14614.5
30	17071.0
31	19734.0
32	22397.0
33	26596.5
34	30796.0
35	36601.5
36	42407.0
37	48142.0
38	53877.0
39	56673.5
40	59470.0
41	63367.0
42	67264.0
43	67794.0
44	68324.0
45	74977.5
46	81631.0
47	86940.0
48	92249.0
49	95654.5
50	99060.0
51	96217.0
52	93374.0
53	92399.0
54	91424.0
55	91876.0
56	92328.0
57	89750.0
58	87172.0
59	79023.0
60	70874.0
61	61926.5
62	52979.0
63	45668.0
64	38357.0
65	32712.0
66	27067.0
67	23274.0
68	19481.0
69	17587.5
70	15694.0
71	12312.5
72	8931.0
73	7566.5
74	6202.0
75	4895.0
76	3588.0
77	3161.5
78	2735.0
79	2267.0
80	1799.0
81	1448.0
82	1097.0
83	878.5
84	660.0
85	466.0
86	272.0
87	198.0
88	124.0
89	95.0
90	66.0
91	56.5
92	47.0
93	39.0
94	31.0
95	37.0
96	43.0
97	31.5
98	20.0
99	18.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00923759561687732
2	3.105074156933553E-4
3	1.5525370784667765E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	7.762685392333882E-5
9	0.0011644028088500823
10	7.762685392333883E-4
11	7.762685392333882E-5
12	3.105074156933553E-4
13	2.3288056177001647E-4
14	2.3288056177001647E-4
15	0.004269476965783636
16	0.0010867759549267435
17	0.0
18	3.8813426961669415E-4
19	7.762685392333882E-5
20	0.0010867759549267435
21	0.0
22	1.5525370784667765E-4
23	5.433879774633717E-4
24	5.433879774633717E-4
25	0.0016301639323901154
26	0.006598282583483801
27	0.006520655729560462
28	0.0038813426961669412
29	0.002872193595163537
30	0.003105074156933553
31	0.007685058538410543
32	0.003648462134396925
33	0.004502357527553652
34	0.003648462134396925
35	0.0031827010108568914
36	0.0070640437070238335
37	0.003648462134396925
38	0.008538953931567271
39	0.004890491797170346
40	0.0031827010108568914
41	0.004657611235400329
42	0.002173551909853487
43	0.0014749102245434375
44	0.00131965651669676
45	0.0013972833706200989
46	0.0015525370784667766
47	0.00263931303339352
48	0.0020959250559301483
49	3.8813426961669415E-4
50	0.0018630444941601318
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1288214.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.037641663273156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16082830057667	47.91543898303885
2	11.65783287551421	13.765019197498665
3	3.217682081574956	5.698930851550714
4	1.337256442030736	3.157938665460568
5	0.6931928021323253	2.0462234127924215
6	0.4123624081841179	1.4606942453867
7	0.2767522153793782	1.1437158684759308
8	0.20053083052542797	0.9471093851999022
9	0.1440959624974672	0.7656377219144924
>10	0.7547283587342393	8.068487624107503
>50	0.06904107215817844	2.8687827286990952
>100	0.06735944597064547	8.335839300543324
>500	0.006881502310031691	2.789808821257968
>1k	0.0014557024117374732	1.0363731940737915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1399	0.10859996863875103	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1371	0.10642641672889754	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1309	0.10161355178565051	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5525370784667765E-4	0.0
5	0.0	0.0	0.0	1.5525370784667765E-4	7.762685392333882E-5
6	0.0	0.0	0.0	1.5525370784667765E-4	2.3288056177001647E-4
7	0.0	0.0	0.0	1.5525370784667765E-4	2.3288056177001647E-4
8	0.0	0.0	0.0	1.5525370784667765E-4	2.3288056177001647E-4
9	0.0	0.0	0.0	1.5525370784667765E-4	2.3288056177001647E-4
10	0.0	0.0	0.0	1.5525370784667765E-4	2.3288056177001647E-4
11	0.0	0.0	0.0	1.5525370784667765E-4	2.3288056177001647E-4
12	0.0	0.0	0.0	1.5525370784667765E-4	4.6576112354003294E-4
13	0.0	0.0	0.0	1.5525370784667765E-4	6.210148313867106E-4
14	0.0	0.0	0.0	1.5525370784667765E-4	6.986416853100494E-4
15	0.0	0.0	0.0	1.5525370784667765E-4	9.315222470800659E-4
16	7.762685392333882E-5	0.0	0.0	1.5525370784667765E-4	9.315222470800659E-4
17	7.762685392333882E-5	0.0	0.0	1.5525370784667765E-4	9.315222470800659E-4
18	7.762685392333882E-5	0.0	0.0	1.5525370784667765E-4	9.315222470800659E-4
19	7.762685392333882E-5	0.0	0.0	1.5525370784667765E-4	0.0010091491010034046
20	7.762685392333882E-5	0.0	0.0	1.5525370784667765E-4	0.0010867759549267435
21	7.762685392333882E-5	0.0	0.0	1.5525370784667765E-4	0.0010867759549267435
22	7.762685392333882E-5	0.0	0.0	2.3288056177001647E-4	0.0010867759549267435
23	7.762685392333882E-5	0.0	0.0	3.105074156933553E-4	0.0011644028088500823
24	7.762685392333882E-5	0.0	0.0	4.6576112354003294E-4	0.0011644028088500823
25	7.762685392333882E-5	0.0	0.0	4.6576112354003294E-4	0.0011644028088500823
26	7.762685392333882E-5	0.0	0.0	5.433879774633717E-4	0.0011644028088500823
27	7.762685392333882E-5	0.0	0.0	8.53895393156727E-4	0.0011644028088500823
28	7.762685392333882E-5	0.0	0.0	0.0012420296627734212	0.0011644028088500823
29	7.762685392333882E-5	0.0	0.0	0.00263931303339352	0.0011644028088500823
30	7.762685392333882E-5	0.0	0.0	0.004657611235400329	0.0011644028088500823
31	7.762685392333882E-5	0.0	0.0	0.011023013257114113	0.0011644028088500823
32	7.762685392333882E-5	0.0	0.0	0.01831993752590796	0.0011644028088500823
33	7.762685392333882E-5	0.0	0.0	0.024141951570158374	0.0011644028088500823
34	7.762685392333882E-5	0.0	0.0	0.031516502692875564	0.00131965651669676
35	7.762685392333882E-5	0.0	0.0	0.041996127972526304	0.00131965651669676
36	7.762685392333882E-5	0.0	0.0	0.05457167830810719	0.00131965651669676
37	7.762685392333882E-5	0.0	0.0	0.07607431684487205	0.00131965651669676
38	7.762685392333882E-5	0.0	0.0	0.11092877425645117	0.00131965651669676
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGATT	65	5.931888E-7	27.076826	29
GTATCAA	1930	0.0	25.076658	1
CTAGCCG	70	3.2175798E-5	21.998215	4
GATATAC	385	0.0	21.713366	1
CGATTTT	85	6.1100236E-6	20.707418	31
GTATTAC	135	2.5993359E-9	19.554728	1
ATACACT	470	0.0	19.189932	4
GTCCTAA	445	0.0	18.78572	1
TATACAC	535	0.0	18.50317	3
CGTATGT	120	3.159912E-7	18.334692	33
AACGCAG	2690	0.0	18.236439	6
AATGCCG	185	2.1827873E-11	17.83639	18
CGGATCG	100	2.4816367E-5	17.601305	26
GGATCGA	100	2.4824647E-5	17.600622	27
GCATATA	125	4.8552465E-7	17.599255	1
AACGTAT	115	4.2703105E-6	17.218666	31
GTATATA	155	1.4400939E-8	17.031536	1
TCAACGC	2875	0.0	16.909931	4
GTAGGAC	755	0.0	16.89929	3
GGTATCA	915	0.0	16.82989	1
>>END_MODULE