FastQCFastQC Report
Thu 2 Feb 2017
SRR4063998_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063998_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814956
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22100.271180284579781No Hit
TATCAACGCAGAGTACTTTTTTTTT16270.1996426800956125No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11990.14712450733536533No Hit
GGTATCAACGCAGAGTACTTTTTTT8400.10307304934254119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.005280888614.2481125
TAGCACT551.9605513E-413.8163514
TCTAGAC551.9615729E-413.8155023
TCAGTAC551.9615729E-413.8155023
AATACTA500.001499976113.2982385
GTATTAC500.00150136513.2966041
TTTTCGT1207.366907E-1012.66809916
CGGTTCT1501.8189894E-1212.66732212
GGTTCTA1501.8189894E-1212.66732213
CGTAACT604.0865265E-412.66732112
TACTATG604.0907634E-412.6657667
TCAATAC604.095004E-412.664213
GCTCTTA1750.012.4825271
ACGGTAT1001.4381658E-712.3506379
TCGGCGT701.089145E-412.21491713
CAGGGGT550.003069333712.0893084
GTATTAG951.0409494E-611.9969371
TTTTTCG1353.6925485E-1011.96505315
GTATTAA1052.722536E-711.7589021
TTCGTCA1406.7484507E-1011.53843618
Produced by FastQC (version 0.11.5)