##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063998_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814956
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25264186041946	32.0	32.0	32.0	32.0	32.0
2	30.832462120654366	32.0	32.0	32.0	32.0	32.0
3	30.864989030082604	32.0	32.0	32.0	32.0	32.0
4	30.89091558317259	32.0	32.0	32.0	32.0	32.0
5	30.79302195455951	32.0	32.0	32.0	32.0	32.0
6	34.457115476172945	36.0	36.0	36.0	32.0	36.0
7	34.39821536377424	36.0	36.0	36.0	32.0	36.0
8	34.347676438973394	36.0	36.0	36.0	32.0	36.0
9	34.49395059365168	36.0	36.0	36.0	32.0	36.0
10	34.18037415516911	36.0	36.0	36.0	32.0	36.0
11	34.48223953195019	36.0	36.0	36.0	32.0	36.0
12	34.285691497455076	36.0	36.0	36.0	32.0	36.0
13	34.374232473900435	36.0	36.0	36.0	32.0	36.0
14	34.267883174060934	36.0	36.0	36.0	32.0	36.0
15	34.19960218711194	36.0	36.0	36.0	32.0	36.0
16	34.198094130235255	36.0	36.0	36.0	32.0	36.0
17	34.13004751176751	36.0	36.0	36.0	32.0	36.0
18	34.120472761719654	36.0	36.0	36.0	32.0	36.0
19	34.14292796175499	36.0	36.0	36.0	32.0	36.0
20	34.11811926042633	36.0	36.0	36.0	32.0	36.0
21	34.10632843981761	36.0	36.0	36.0	32.0	36.0
22	34.08444382273399	36.0	36.0	36.0	32.0	36.0
23	34.02440990679251	36.0	36.0	36.0	32.0	36.0
24	34.007524332602	36.0	36.0	36.0	32.0	36.0
25	33.61336931073579	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	10.0
6	27.0
7	16.0
8	44.0
9	70.0
10	63.0
11	25.0
12	37.0
13	38.0
14	84.0
15	151.0
16	237.0
17	319.0
18	382.0
19	564.0
20	816.0
21	1150.0
22	1851.0
23	2844.0
24	4298.0
25	6388.0
26	8962.0
27	11994.0
28	16228.0
29	22396.0
30	29068.0
31	39746.0
32	56500.0
33	78865.0
34	168741.0
35	363037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.81552848632305	16.87070888353942	11.344444458084086	25.96931817205344
2	16.633350390285145	19.7907271538083	37.74224859709639	25.83367385881016
3	18.723917015462355	24.02487523728537	28.70947435845521	28.541733388797063
4	12.497883253776399	15.549801823469501	36.005301068803455	35.947013853950644
5	14.47059213327754	36.793426440757834	33.782753978704164	14.953227447260463
6	34.05779938221986	35.592554730730576	17.089462761995108	13.260183125054459
7	30.130441079366793	30.44512956967208	20.876586636294235	18.54784271466689
8	28.159607401242702	32.91402450550772	19.533703051772857	19.392665041476715
9	27.672438663581172	13.844199051027237	18.312964588461327	40.170397696930266
10	15.64690745065107	26.99157526156959	32.02124968999615	25.340267597783196
11	37.151949085123306	20.858378266890597	22.758232908060542	19.231439739925552
12	24.738447938217114	23.42654380482894	29.234896527168143	22.60011172978581
13	29.841134588066005	19.47823289701312	25.17192309534012	25.508709419580754
14	23.742628162231387	19.35777372034228	25.47196337993927	31.427634737487057
15	25.122408187720385	27.098373456699175	22.45128227794639	25.32793607763405
16	25.624207270579657	25.58110980277076	24.039423746336407	24.755259180313175
17	23.990865393469495	25.5993664708374	25.467135674690137	24.942632461002965
18	24.566209933516223	24.944433119579926	26.32777214263435	24.161584804269502
19	25.568207633242835	24.740071639166512	25.504967710800763	24.18675301678989
20	25.8429753486967	24.214618858971555	25.099162734951207	24.843243057380533
21	26.66674034288914	24.13682165688001	24.602946557724092	24.59349144250676
22	25.757977645645912	24.293597990023798	24.940135422883372	25.008288941446917
23	24.671863794001197	24.106099010679163	25.391872243017282	25.830164952302358
24	24.836433564029136	24.795787550285628	25.420950257138227	24.94682862854701
25	24.982501209565513	24.44452085241827	25.338739743252837	25.234238194763382
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	162.0
1	162.0
2	286.5
3	411.0
4	411.0
5	411.0
6	1019.0
7	1627.0
8	1627.0
9	1627.0
10	1665.5
11	1704.0
12	1704.0
13	1704.0
14	1877.5
15	2051.0
16	2051.0
17	2051.0
18	3539.0
19	5027.0
20	5027.0
21	5027.0
22	8163.5
23	11300.0
24	11300.0
25	11300.0
26	17004.5
27	22709.0
28	22709.0
29	22709.0
30	28988.5
31	35268.0
32	35268.0
33	35268.0
34	43060.5
35	50853.0
36	50853.0
37	50853.0
38	58749.5
39	66646.0
40	66646.0
41	66646.0
42	75894.0
43	85142.0
44	85142.0
45	85142.0
46	95226.5
47	105311.0
48	105311.0
49	105311.0
50	108737.0
51	112163.0
52	112163.0
53	112163.0
54	104688.0
55	97213.0
56	97213.0
57	97213.0
58	89125.5
59	81038.0
60	81038.0
61	81038.0
62	70729.5
63	60421.0
64	60421.0
65	60421.0
66	49103.0
67	37785.0
68	37785.0
69	37785.0
70	28367.5
71	18950.0
72	18950.0
73	18950.0
74	14336.5
75	9723.0
76	9723.0
77	9723.0
78	7507.0
79	5291.0
80	5291.0
81	5291.0
82	3637.0
83	1983.0
84	1983.0
85	1983.0
86	1543.5
87	1104.0
88	1104.0
89	1104.0
90	788.0
91	472.0
92	472.0
93	472.0
94	313.0
95	154.0
96	154.0
97	154.0
98	301.0
99	448.0
100	448.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04135192574813855
2	0.004785534433760841
3	0.0011043541000986556
4	0.003190356289173894
5	0.006748830611714006
6	0.012638719145573503
7	0.022332494024217258
8	0.034603095136424546
9	0.04797805034873048
10	0.05521770500493278
11	0.051536524671270595
12	0.06024865146093776
13	0.06049406348318192
14	0.06613853999479727
15	0.05742641320513009
16	0.0641752438168441
17	0.05779453123849631
18	0.07632313891792931
19	0.07325548863987749
20	0.07828643509588248
21	0.0714148984730464
22	0.07583231487344103
23	0.08098596734056808
24	0.07460525476222028
25	0.07534149082895272
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	814956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.8213033419025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41856620267666	45.53860966902804
2	13.829555827594461	16.269449968374182
3	4.1807751217620135	7.377559249243282
4	1.721447003663733	4.050310255580544
5	0.8355742945971381	2.457478452359723
6	0.504090719885392	1.7790763887729988
7	0.2963665819946326	1.2202868033936374
8	0.20095152510389733	0.9456184492123423
9	0.15519707910007918	0.8216005020770696
>10	0.7220378300657622	7.584124634462802
>50	0.06918738300901946	2.9140059906421634
>100	0.06205736532939924	7.096740965174544
>500	0.0035641054351221784	1.3240952208085828
>1k	6.289597826686198E-4	0.6210434508700775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2210	0.271180284579781	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1627	0.1996426800956125	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1199	0.14712450733536533	No Hit
GGTATCAACGCAGAGTACTTTTTTT	840	0.10307304934254119	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.454120222441457E-4
2	0.0	0.0	0.0	0.0	2.454120222441457E-4
3	0.0	0.0	0.0	0.0	2.454120222441457E-4
4	0.0	0.0	0.0	0.0	2.454120222441457E-4
5	0.0	0.0	0.0	0.0	2.454120222441457E-4
6	0.0	0.0	0.0	0.0	2.454120222441457E-4
7	0.0	0.0	0.0	0.0	2.454120222441457E-4
8	0.0	0.0	0.0	0.0	3.6811803336621855E-4
9	0.0	0.0	0.0	0.0	3.6811803336621855E-4
10	0.0	0.0	0.0	0.0	3.6811803336621855E-4
11	0.0	0.0	0.0	0.0	3.6811803336621855E-4
12	0.0	0.0	0.0	1.2270601112207284E-4	7.362360667324371E-4
13	0.0	1.2270601112207284E-4	0.0	1.2270601112207284E-4	8.5894207785451E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	0.0052808886	14.248112	5
TAGCACT	55	1.9605513E-4	13.816351	4
TCTAGAC	55	1.9615729E-4	13.815502	3
TCAGTAC	55	1.9615729E-4	13.815502	3
AATACTA	50	0.0014999761	13.298238	5
GTATTAC	50	0.001501365	13.296604	1
TTTTCGT	120	7.366907E-10	12.668099	16
CGGTTCT	150	1.8189894E-12	12.667322	12
GGTTCTA	150	1.8189894E-12	12.667322	13
CGTAACT	60	4.0865265E-4	12.667321	12
TACTATG	60	4.0907634E-4	12.665766	7
TCAATAC	60	4.095004E-4	12.66421	3
GCTCTTA	175	0.0	12.482527	1
ACGGTAT	100	1.4381658E-7	12.350637	9
TCGGCGT	70	1.089145E-4	12.214917	13
CAGGGGT	55	0.0030693337	12.089308	4
GTATTAG	95	1.0409494E-6	11.996937	1
TTTTTCG	135	3.6925485E-10	11.965053	15
GTATTAA	105	2.722536E-7	11.758902	1
TTCGTCA	140	6.7484507E-10	11.538436	18
>>END_MODULE