FastQCFastQC Report
Thu 2 Feb 2017
SRR4063998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814956
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC1107.2759576E-1225.9960483
TGAACGG654.9332797E-420.30585110
CGCCCCT1201.4322723E-820.16365
TAATACT1452.9649527E-1019.721144
CTATACT1252.2891982E-819.3570584
TAATACC708.1285264E-418.8542754
GTGTAGA2000.018.6971571
GTATCAA17250.018.617461
CTAGCGG1801.4551915E-1118.33504729
TACGAAT1801.4551915E-1118.33392141
GTGCTAT1203.1638774E-718.3305471
CGCCGGT1801.4551915E-1118.3305477
TAAGACC1203.1638774E-718.3305474
CGTCGTA1951.8189894E-1218.04964610
AGACCGT750.001291696617.5973246
TAGGACG1509.600626E-917.5973244
TCTATAC1002.4846473E-517.5973243
CGCAATA1903.274181E-1117.37110936
TCTAGCG1903.274181E-1117.37004528
GACGTGA1154.2756237E-617.2147737
Produced by FastQC (version 0.11.5)